Objective To observe the changes of ambulatory arterial stiffness index (AASI) and target organ damage (TODs) after antihypertensive treatment in hypertensive patients .Methods A total of 300 cases of newly diagnosed hypertension were screened out and after strictly controlling blood pressure ,completed the ambulatory blood pressure and target organ damage inspection by 1‐year follow‐up .The dynamic changes of AASI and target organ damage were observed .Results After 1‐year anti‐hypertensive therapy ,AASI showed the gradually descending trend ,compared with before treatment ,began to decline at 6 months , decreased significantly after 1 year ;AASI showed the independent positive correlation with the intima‐media thickness(IMT) of ca‐rotid artery ,urine microalbumin(UMA) ,renal vascular resistance index(RRI) and left ventricular mass index(LVMI) ,and inde‐pendent negative correlation with endogenous creatinine clearance rate(Ccr) before and after treatment ;the multiple linear regres‐sion analysis showed that IMT ,UMA ,RRI ,Ccr and LVMI had significant correlation with AASI .After 1 year of therapy ,IMT , UMA ,RRI ,Ccr ,LVMI had shown some improvement(P<0 .05) .Conclusion AASI can be used as an indicator for evaluating arte‐riosclerosis and predicting the target organ damage in hypertension .

Objective To analyze the clinical application of donor specific antibodies (DSAs) detected by a single antigen Luminex virtual crossmatch,and to discuss the treatment of DSA and the impact of DSA on renal function.Methods Serum from living-relative renal recipients before and after transplantation was investigated using a Luminex single antigen assay.The relation between DSA and renal acute rejection as well as renal function was analyzed.Results A total of 30 patients and 173 serum samples were tested,including 47 serum samples before transplantation,and 126 after transplantation.DSA was positive in one patient before transplantation,and 8 patients after transplantation.Three of the patients positive for DSA were treated by Bortezomib,3 by addition of MMF,2 by addition of CNI,1 by addition of Sirolimus.The MFI of DSA in one of the patients treated by Bortezomib was decreased to below 1000,while that in the other two decreased by more than 50 ％.The renal eGFR at the time with and without DSA was (1.50 ± 0.59) and (1.23 ± 0.38)ml/s respectively (P＜0.05).Conclusion Dynamic monitoring of single bead antigen antibody DSA conduces to direct the adjustment of immunosuppressant.The appearance of DSA contributes to the declination of renal function.Application of Bortezomib decreased the MFI of DSA.

Objective To establish an automatic warning program which accommodates to the test procedures in medical laboratory centerand and to put into operation.Methods Based on laboratory information management system (LIS),automatic warning program was established,which composed of maintenance of warning rules,feedback of vulnerabilities in rules,solutions of feedback targets and evaluation of ride-effectiveness.The results of performance were assessed after operation for six months in Guangzhou Kingmed Medical Laboratory Center.Results An automatic warning program containing 13 kinds of rule templates was successfully established.The multi-point warning program via asynchronous structure for six specialized testing procedures was realized.A total of 1 523 warning rules were included in the rule bank and 24 000 reports were verified on average each day.The average passing rate of reports was 70.2％ and the passing rate of single test was 83.9％.The approval time for reports was reduced by 10 to 40 minutes compared with that without using the automatic warning program.The efficiency for report approval was improved by 25 percent.Conclusion Automatic warning program may ensure the high quality of test reports,ease pressure on staff and improve work efficiency.