Objective To explore the effects of post-stroke depression on working memory,aiming to find a new method for alleviating the dysfunction in post-stroke depression patients.Methods Eighteen patients with poststroke depression and 20 stroke survivors with no depression were recruited as the experimental and control groups respectively.Both groups' working memory was tested early every morning.The two groups' performance was compared in terms of each working memory test item and their results were correlated with their scores on the Hamilton depression scale (HAMD).Results The average HAMD score was 21.78 ± 3.21 for the experimental group and 5.06 ±2.35 for the control group.Compared with the control group,the experimental group was significantly different in its performance counting backward,in its word memory span and its calculation task span,as well as in successive subtraction.At the same time,compared with the control,the experimental group was significantly better in total reaction number and the percentage of number of right reaction of the number of total reaction of Digit Subtraction Test.The HAMD scores of the experimental group were negatively correlated with their performance in counting backward,their word memory span,their successive subtraction performance and their calculation task span.Conclusion Poststroke depression has an obvious negative effect on working memory.

BACKGROUND:Previous study has confirmed that seasonal variation and surfactant associated protein A(SP-A)and interleukin-6(IL-6)exhibit a significant role in lung immune and defense function.However,the effect mechanism of them remains unclear.OBJECTIVE:By observing the changes of SP-A and IL-6 of male rats in autumn and winter.to explore the effects of seasonal changes on the nonspecific immunity of lung of normal rats,and to provide new ideas for the experimental basis to the cognition of pathology and physiology mechanism for seasonal attack of respiratory system diseases.METHODS:Male SD rats were purchased from each seasons and divided into groups in chronological order All rats were housed with normal forage and drank freely under room temperature.Then the rats were sacrificed by decapitation before 18 o'clock at corresponding solar term and the lung tissues were analyzed by RT-PCR.RESULTS AND CONCLUSION:Compared to the other groups.the expression of SP-A and IL-6 in the Winter Begins group were smaller.which demonstrated that immune function of lung was existed the seasonaI rhythm of lower in winter and higher in autumn.The SP-A and IL-6 are main material basis of immune function.

Objective:To observe the effects of Chaihu Jiedu decoction on human hepatic stellate LX-2 cells,and to explore the potential molecular mechanisms.Methods:The wistar rats were divided into the experimental group and the control group,respectively with Chaihu Jiedudecoction and saline lavage,then centrifugal and get the drug-containing serum and control serum.At the same time,trsuscitate cells.When we got the expected number of cells,we divided into the experimental group and the control group.The human hepatic stellate cells (LX-2) were with the drug serum for 24 h,36 h,48 h,72 h.Then the cell proliferation inhibition rate was measured by CCK-8,the apoptosis were detected by flow cytometry (Annexin V-FITC,PI staining method).Results:ChaihuJiedu decoction could inhibit proliferation of LX-2 cells 24 hours after dosing.The in hibition rate in 36 h,48 h,72 h were 0.37 ％,0.46 ％,0.44 ％ respectively.It could prevent LX-2 cells into proliferation and induce the apoptosis of LX-2 cells.The apoptosis rates in 48 h,72 h were (9.80±0.95)％,(36.40± 5.09)％ respectively,and there are difference of statistical significance(P＜0.05).Conclusion:Chaihu Jiedu decoction can inhibit the proliferation of LX-2 cells proliferation,and induce apoptosis,so as to interfere with course of the liver fibrosis.

As the most common organ-specific autoimmune disease,autoimmune thyroiditis (AIT) is characterized by intrathyroidal lymphocyte infiltration and specificthyroid autoantibodies.Modern medicine is short of effective etiological treatment at present,and has no specific medicine foreuthyroidism of AIT patients,who have been followed-up passively.Chinese medicine treatmenthas the characteristics of multiple-targets,multiple-links and multiple-pathways,which plays a special superiorityrole in the prevention and treatment of AIT.The purpose of this article was to sort out and set forthmore Chinese medicine pharmacology on AIT recently,which provided evidence for further clinical apply.

OBJECTIVETo characterize the expressions of peroxiredoxin 1 (Prx1), peroxiredoxin 6 (Prx6) and glial fibrillary acidic protein (GFAP) in human brain astrocytoma and explore their clinical significance.

METHODSThe protein and mRNA expression levels of Prx1, Prx6 and GFAP in human brain astrocytoma and normal brain tissue specimens were determined by Western blotting, RT-PCR and immunohistochemistry.

RESULTSThe protein and mRNA expressions of Prx1 and Prx6 increased significantly in the order of normal brain tissue, grade II astrocytoma, grade III astrocytoma and grade IV astrocytoma (P<0.05). The protein and mRNA expressions of GFAP decreased significantly in grade III and IV astrocytoma compared with those in grade II astrocytoma and normal brain tissues (P<0.05).

CONCLUSIONPrx1 and Prx6 may play important roles in the invasion and malignant development of human brain astrocytoma, and may serve as biomarkers for evaluating the invasiveness, malignancy and prognosis of the tumor as well as potential molecular targets in astrocytoma therapy.

Adolescent ; Adult ; Aged ; Astrocytoma ; metabolism ; pathology ; Brain Neoplasms ; metabolism ; pathology ; Child ; Child, Preschool ; Female ; Glial Fibrillary Acidic Protein ; metabolism ; Humans ; Male ; Middle Aged ; Peroxiredoxin VI ; metabolism ; Peroxiredoxins ; metabolism ; Young Adult

Objective To investigate mental health literacy (MHL) of community medical staffs in district and town of Zhongshan city,and to provide data to improve MHL of community medical staffs.Methods Totally 352 medical staffs who were not psychiatric physician and 81 psychiatric physician were selected.The Chinese Mental Health Knowledge Awareness Questionnaire (published by Ministry of Health) was used to investigate the awareness rate of mental health knowledge.Five vignettes of two schizophrenia,one bipolar disorder,one depression and one obsessive compulsive disorder,each with 8 related questions,were used to investigate the recognition and response ability.Results The average awareness rate of the two groups was more than 80.0% (87.6%,91.6%).For the item 2(75.6%,88.6%),6(74.1%,62.6%),19(36.5%,65.6%),and 20(74.1%,86.2%),the awareness rates were lower than 80.0%,and there were significant differences between the two groups (x2=8.45,4.92,27.48,6.99,all P<0.05).In vignettes survey,the correct rate was lower in the staffs who did not engaged in the mental health work than those in the other group,the difference was statistically significant(64.6% vs.75.9%,P<0.001).For both of the two groups the correct rate of depression was the lowest(x2=44.46,P<0.001).There was statistically significant difference between the total (x2=141.17,P<0.001).Conclusion The awareness rate of mental health knowledge has reached the national standards for community medical staffs,but they have to improve for some knowledge point.Their recognition and response ability for mental illness should be improved.

Objective To explore the effects of stomach tube connector for preventing overfall and provide a theoretical basis for generalization of the new-type connector. Methods This study selected 157 patients required for long-term indwelling gastric tube in the First People's Hospital of Xuzhou from May 2016 to January 2017. Patients were divided into experimental group (n=78, stomach tube for preventing overfall) and control group (n=79, conventional stomach tube) with the random number table. This study recorded the success rate of indwelling gastric tube and adverse reactions of patients between two groups. The gastric tube was removed two months later. And then, the bacterial cultures were carried out. The characters of gastric tube and the injury of nasal mucosa were observed. Besides, the hand contamination of nurses between two groups and the the patients' satisfaction was investigated. Results The success rate of indwelling gastric tube, incidence of adverse reactions and injuries of nasal mucosa, and change of characters of gastric tube of experimental group and control group was 91.03%(71/78) vs 88.61%(70/79),7.69%(6/78) vs 7.59%(6/79), 15.38%(12/78) vs 17.72% (14/79),8.97% (7/78) vs 10.13% (8/79) with no significant differences (P> 0.05). The positive rate of bacterial cultures of gastric tube was 10.13% in control group, while there was no pathogenic bacteria in experimental group with a significant difference (P<0.05). The hand contamination rate of nurses was 8.97%(7/78) in experimental group significantly lower than that [24.05% (19/79)]in control group (P<0.01). The patients' satisfaction degree was 91.03% in experimental group which was higher than that in control group (69.62%)with a significant difference (P< 0.05). Conclusions The stomach tube connector for preventing overfall can effectively prevent nasogastric feeding overfall and avoid bacteria contamination in stomach tube connector and hand contamination of nurses. The operator was simplicity and safe with high patients' satisfaction. It is worth to be used widely in clinical application.

Objective To explore the application effects of problem-based learning (PBL) combined with WeChat video teaching mode of the practice nurses teaching in digestive endoscope department, so as to provide ideas and methods for practice nurses teaching of digestive endoscopy. Methods A total of 90 practice nurses who entered the gastroenterology department of the Central Hospital of Enshi Autonomous Prefecture our Institute of digestive endoscopy from January to December 2017 were selected as the research subjects by cluster sampling. The 45 practice nurses from January to June 2017 were recruited as the control group, and the other 45 from July to December 2017 were recruited as the experimental group. The control group used the conventional teaching method. The experimental group used PBL combined with WeChat video teaching mode. After 1 month's practice, the theoretical knowledge, teaching satisfaction and critical thinking ability were compared between the two groups. Results The theoretical knowledge score of the control group was (83.49±6.82), which was lower than that of the experimental group (89.97±4.38), and the difference was statistically significant (t=-5.363, P＜0.001). The satisfaction rate of the control group was 55.56%, which was lower than that of the experimental group (88.89%), and the difference was statistically significant (χ2=12.461, P＜ 0.001). The total score and the scores of each dimension of critical thinking ability of control group were all lower than those of the experimental group, and the differences were statistically significant (P＜ 0.05). Conclusions The application of PBL combined with WeChat video teaching mode in the teaching practice of digestive endoscopy nurses can improve the academic knowledge scores of practice nurses, improve their satisfaction with teachers, and help the training of critical thinking ability, which is worthy of clinical reference and promotion.

Objective To explore the clinical value of genetic screening for early identification of WAS gene-related disorders in newborns.Methods This was a retrospective study.Neonatal Genome Project from Children's Hospital of Fudan University collected 5 800 high-risk newborns in the neonatal intensive care unit to study the patients' genetic causes using high-throughput sequencing from January 2016 to December 2017.Eleven newborns (all were boys) with pathogenic or likely pathogenic variants in WAS gene were enrolled.Data of clinical characteristics,gene variants and genotype-phenotype correlation were collected and summarized.Results Eleven patients included 5 cases with Wiskott-Aldrich syndrome (WAS) and 6 cases with X-linked thrombocytopenia (XLT).Two patients with WAS developed clinical manifestations in the early neonatal period,and 3 patients in 5-8 weeks after birth.Three neonates with XLT were hospitalized for other diseases in the first place.Their platelet count was found to be reduced after admission to hospital,and diagnosis was made after genetic testing.Eleven pathogenic or likely pathogenic variants in WAS gene were identified.Among them,7 were first reported in this study,including 2 frame shift variants c.138delG and c.388_390del,4 splicing variants c.1453+ 1G＞A,c.734+ 1G＞C,c.135G＞A and c.1453+3G＞C,and 1 missense variant c.1118C＞T.The other 4 reported variants were c.777+ 1G＞A,c.107_ 108delTT,c.436delC and c.1509_*3delAGTG.Conclusions The clinical features of WAS gene-related disorders in neonatal period lack specificity.Genetic screening in newborns plays an important role in the early diagnosis of diseases and provides providing evidence for the early intervention.

Objective:To evaluate the efficacy and safety of unrelated umbilical cord blood stem cell transplantation (UCBT) with reduced-intensity conditioning regimens in the treatment of hyper-IgM syndrome (HIGM) caused by CD40 ligand gene (CD40LG) mutation.Methods:Clinical features, laboratory data and treatment prognosis of 3 patients with HIGM caused by CD40LG mutation treated with UCBT in Children′s Hospital of Fudan University from May 2018 to August 2019 were analyzed retrospectively. The literature search was conducted with "CD40 ligand deficiency" and "hematopoietic stem cell transplantation" as keywords at China National Knowledge Infrastructure, Wanfang, Weipu and Pubmed databases (up to February 2021) to summarize donor selection, stem cell source, conditioning regimen and prognostic factors of this disease.Results:Three boys with recurrent respiratory tract infection were diagnosed as HIGM with CD40LG mutation. The age of UCBT was 1.0, 1.4 and 0.5 years respectively. Reduced-intensity conditioning regimen including busulifan, fludarabine and cyclophosphamide were used in all patients. Human leucocyte antigen matching of patients and umbilical cord blood was 8/10, 10/10 and 9/10 respectively. All patients achieved complete donor chimerism 14 days after UCBT. All patient suffered grade Ⅰ acute skin graft-versus-host disease without other severe complications. Up to the last follow-up, their disease-free survival time were 33, 18, 18 months after transplantation respectively. No reports were found in Chinese journals, while 24 publications were found in English journals. According to the literature, 258 HIGM patients with CD40LG mutation were treated with hematopoietic stem cell transplantation (HSCT). Matched sibling donors (30.6%(79/258)) and unrelated donors (40.3% (104/258)) were main donor types. Bone marrow (50.8%(131/258)) was the main source of grafts, myeloablative conditioning (66.7% (172/258)) was the main conditioning regimen, and the overall survival rate after transplantation was 70.9% (183/258). Lung injury and liver complications before transplantation were adverse factors affecting prognosis. Among the 14 patients who received UCBT, 2 patients suffered from engraftment failure, 2 patients had mixed chimerism and 3 patients died after transplantation.Conclusions:UCBT is safe and effective in the treatment of HIGM caused by CD40LG mutation. Reduced-intensity conditioning regimen is worthy of further study.