0.05) ; Compared with group A and B , contents of brain water and MDA were significantly increased at 3 hours after ACCI (P

Objective To investigate the effects of passaging on the proliferation,differentiation and apoptosis of neural stem cells.Methods Neural stem cells both from prime culture(P0) and twice-passaged culture(P2) were tested by enzyme immunoassay for proliferation and fluorescent activated cell sorting for apoptosis by Annexin V-FITC and propidium iodide.After being enduced to differentiation for 7 days,they were detected by the neuronal marker MAP2 and the astrocyte marker GFAP with nuclei staining by Hoechst 33342 additionally.The ratios of MAP2+/Hoechst+ and GFAP+/Hoechst+ were calculated.Results The absorbance of NSCs from P0 and P2 is 1.1899?0.0485(n=13)and 0.3526?0.1053(n=11)(P=0.000).Analyses of apoptosis using cytoflurometry are 40.91%?12.74% for P0(n=8)and 24.31%?13.01% for P2(n=9,P=0.018).The ratios of MAP2+/Hoechst+ and GFAP+/Hoechst+ of NSCs from P0 were 58.81%?9.69%(n=10)和22.85%?4.36%(n=10) while the ones for P2 were 27.84%?5.70%(n=10)and 49.32%?6.95%(n=10,P=0.000).Conclusions By passaging the proliferation and apoptosis of NSCs decreases and they differentiate into less neurons and more astrocytes.

Objective To evaluate the efficacy of combined various surgical procedures for the treatment of post-traumatic epilepsy.Methods According to the preoperative primary location of epileptogenic focus and the monitoring results of intraoperative electrocorticography and depth electrodes,21 seizure patients were treated with various surgical procedures: 7 cases with epileptogenic focus resection,8 cases with epileptogenic focus resection combined with multiple subpial transection(MST),4 cases with anterior temporal lobectomy combined with amygdalo-hippocampotomy,1 case with selective amygdalo-hippocampotomy under neuronavigation,and 1 case with anterior callosotomy combined with MST and bipolar coagulation on funtional cortexes. Results All the patients were followed up from 3 months to 10 years postoperatively,and the surgical outcomes were such as the followings: satisfactory outcome in 9 cases(42.9%),marked improvement in 7 cases(33.3%),improvement in 3 cases(14.3%),slight improvement in 1 case(4.8%) and no improvement in 1case(4.8%).There was no died cases caused by the surgical procedures.Eight patients had temporary dysfunction after operation,but all recovered in four weeks after surgery.Conclusion The combined various surgical procedures are effective and safe for the treatment of post-traumatic epilepsy.

Objective To investigate the effects of electrocorticography(ECoG) monitoring in microsurgical treatment of intracranial cavernous hemangiomas(ICH).Methods Fifteen cases of ICH with secondary epilepsy were investigated from 2000 to 2005.They all had been performed ECoG monitoring during operation for determining the precise position and scope of focal epileptogenicity.Results Follow-up visit found 10 cases cured without drugs,4 cases total controlled with 1/4~1/3 quantity of premedicant,1 case had epileptic seizure by chance with drugs.Conclusion ECoG monitoring can provide the direction during the surgical procedure,and controls the postoperative epileptic seizure.