Objective To determine the optimal injection dose of ozone for the treatment of lumbar intervertebral disc protrusion.Methods A total of 240 patients with lumbar intervertebral disc protrusion were randomly and equally divided into four groups,with 60 patients in each group.Under CT guidance intervertebral injection of ozone was performed.The injection dose of ozone(50 ug/ml)used for patients in group A,B,C and D was 10 ml,20 ml,30 ml and 40 ml respectively.Immediately after the procedure CT scanning was made to check the result. A follow-up lasting 6-12 months was carried out.Clinical observation,including the ablation degree of the nucleus puiposus,the therapeutic results and the adverse reactions,was conducted.The results were statistically analyzed and compared among the four groups.Results A significant difference in the therapeutic effect existed between group A(10 ml)and other three groups(P＜0.05),while no significant difference in the therapeutic effect existed among group B(20 ml),group C(30 ml)and group D(40 ml),with P＞0.05.The occurrence of complications was increasing with the injection dose used.Conclusion The optimal injection dose of ozone for the treatment of lumbar intervertebral disc protrusion is 20 ml.

Objective To explore the influencing factors of perioperative myocardial injury in coronary heart disease patients with PCI.Methods Clinical materials of 87 coronary heart disease patients with PCI were retrospectively analyzed,including 23 stable angina pectoris patients (SAP) and 64 unstable angina pectoris patients (SAP).The general information,PCI operation related data,the perioperative myocardial injury and myocardial infarction were compared between two groups.The influencing factors of perioperative myocardial injury in coronary heart disease patients with PCI were analyzed by Logistic regression.The level of blood fat was compared between patients with administration of rosuvastatin >1 month and ≤ 1 month.Results There was no significant difference of general information between two groups (P>0.05).In the SAP group,the number of SAP patients with stent implantation was significantly higher than that of UAP group (P<0.05).There was no significant difference between the two groups in myocardial injury,myocardial infarction,and the level of hs-CRP after PCI (P>0.05).The incidence of myocardial damage in patients with PCI during perioperative period was associated with apolipoprotein A,preoperative statin use,preoperative statin dose therapy,and total length of the stent (P<0.05).At the time of on admission and preoperative,TC and LDL levels of patients with use of statins over one month before operation on admission was significantly lower than patients with use of statins less than one month (P<0.05).Conclusion The loading of apolipoprotein A expression,use of rosuvastatin before PCI,use of statin over 1 month are closely related with the decrease of myocardial injury rate in perioperative period of PCI,and the total length of intra-coronary stent implantation is associated with increasing incidence of myocardial damage.

Objective To explore the influencing factors of perioperative myocardial injury in coronary heart disease patients with PCI.Methods Clinical materials of 87 coronary heart disease patients with PCI were retrospectively analyzed,including 23 stable angina pectoris patients (SAP) and 64 unstable angina pectoris patients (SAP).The general information,PCI operation related data,the perioperative myocardial injury and myocardial infarction were compared between two groups.The influencing factors of perioperative myocardial injury in coronary heart disease patients with PCI were analyzed by Logistic regression.The level of blood fat was compared between patients with administration of rosuvastatin >1 month and ≤ 1 month.Results There was no significant difference of general information between two groups (P>0.05).In the SAP group,the number of SAP patients with stent implantation was significantly higher than that of UAP group (P<0.05).There was no significant difference between the two groups in myocardial injury,myocardial infarction,and the level of hs-CRP after PCI (P>0.05).The incidence of myocardial damage in patients with PCI during perioperative period was associated with apolipoprotein A,preoperative statin use,preoperative statin dose therapy,and total length of the stent (P<0.05).At the time of on admission and preoperative,TC and LDL levels of patients with use of statins over one month before operation on admission was significantly lower than patients with use of statins less than one month (P<0.05).Conclusion The loading of apolipoprotein A expression,use of rosuvastatin before PCI,use of statin over 1 month are closely related with the decrease of myocardial injury rate in perioperative period of PCI,and the total length of intra-coronary stent implantation is associated with increasing incidence of myocardial damage.

Objective To assess differential expression profiles of microRNAs(miRNAs) in HaCaT human keratinocytes before and after p53 gene silencing,and to make a functional analysis of target genes.Methods Lentivirus-mediated RNA interference (RNAi) was used to silence p53 gene in HaCaT cells.Total RNA was extracted using Trizol reagent.Then,miRNAs were isolated by polyethylene glycol (PEG) and subjected to fluorescent labeling using T4RNA ligase followed by hybridization to a mammalian miRNA chip.Microarrays were scanned by a GenePix 4000B microarray scanner and fluorescence ratios were determined with the GenePix Pro 6.0 software.The TargetScan software was used to predict target genes of differentially expressed miRNAs (＞2-fold difference in expression level),and the top 20 target genes with the highest enrichment score were selected for each miRNA and subjected to functional analysis and pathway analysis through the KEGG signaling database.Results Totally,53 differentially expressed miRNAs,including 12 down-regulated and 41 up-regulated miRNAs,were identified in HaCaT cells after p53 silencing as compared to those before p53 silencing.Of these 53 differentially expressed miRNAs,5 (hsa-miR-141-3p,hsa-miR-15a-5p,hsa-miR-27a-3p,hsa-miR-130b-3p,hsa-miR-19a-3p) showed a more than 200-fold increase in expression,and 4 (hiv1-miR-TAR-3p,hsa-miR-630,hsa-miR-1246,hsa-miR-1275) experienced a more than 4-fold decrease in expression in HaCaT cells after p53 silencing.Functional analysis and pathway analysis revealed that some target genes of these differentially expressed miRNAs were involved in the mitogen-activated protein kinase (MAPK) signaling pathway,metabolic pathways,and tumor invasion.Conclusion Nine miRNAs,including hsa-miR-141-3p,may be involved in p53-mediated molecular regulation.

Objective To study the bowel-cleansing efficacy, patient security and mucosal injury of low-volume PEG plus ascorbic acid regimen. Methods Five hundred patients referred for colonoscopy were enrolled and randomly divided into two groups. Group A received low-volume PEG regimen, Group B received sodium phosphate (NaP) regimen for bowel preparation. Patients of the two groups drank solution 5 h before colonoscopies, serum creatinine and electrolyte were monitored at 5 h and 3 h before colonoscopies. The bowel-cleansing efficacy was rated during colonoscopy. All mucosal injuries observed during colonoscopy were biopsied and histopathologically reviewed. Results The patients of group A completed bowel preparation of 233 cases, completed colonoscopy 226 Cases, group B completed bowel preparation 238 cases, completed colonoscopy 210 cases. There was no significant difference in bowel cleansing between the groups (P > 0.05). Group A reported less incidence rate of the mucosal injuries than Group B. Group A reported better patient security than Group B at the same time. Conclusion Compared with sodium phosphate (NaP) regimen low-volume Polyethylene Glycol (PEG) plus ascorbic acid regimen exhibited equivalent bowel-cleansing efficacy and less incidence rate of the mucosal injuries and better patient security.

Objective To investigate the effects of aquaporin 3 (AQP3) and phospholipase D2 (PLD2) on the proliferation and apoptosis of a human cutaneous squamous cell carcinoma cell line A431.Methods Three small interfering RNAs (siRNAs) were constructed targeting the AQP3 and PLD2 genes separately,and transfected into A431 cells using liposomes.Then,fluorescence quantitative PCR was performed to find the most efficient siRNAs.Western blot was conducted to detect the protein expression levels of AQP3 and PLD2 in A431 cells after transfection with the selected AQP3-siRNA and PLD2-siRNA.Some A431 cells were divided into five groups:normal control group without any treatment,transfection reagent group treated with the oligofectamine reagent only,negative control group transfected with the negative control siRNA,AQP3-siRNA group transfected with the selected AQP3-siRNA,PLD2-siRNA group transfected with the selected PLD2-siRNA.After additional culture,cell counting kit-8 assay was performed to evaluate the proliferation of A431 cells,flow cytometry to detect the apoptosis of A431 cells after annexin V-fluorescein isocyanate/propidium iodide double-staining.Statistical analysis was carried out by the paired t test.Results The transfection with AQP3-siRNA and PLD2-siRNA induced a significant decrease in the mRNA and protein expressions of AQP3 and PLD2 respectively in A431 cells when compared with the untransfected cells.Compared with the negative control group,the proliferation of A431 cells was significantly decelerated at 24,48 and 72 hours after transfection in the AQP3-siRNA group (t =24.10,11.00,9.54,respectively,all P ＜ 0.01) and PLD2-siRNA group (t =30.47,7.02,8.73,respectively,all P ＜ 0.01).A significant increase was observed in the apoptosis of A431 cells at 48 and 72 hours after transfection with AQP3-siRNA (t =11.36,20.91,respectively,both P ＜ 0.01),and at 72 hours after transfection with PLD2-siRNA (t =4.86,P ＜ 0.05) compared with the negative control group.Conclusion The down-regulation of AQP3 and PLD2 expressions by siRNA can inhibit the proliferation,but induce the apoptosis,of A431 cells.

Objective To assess the effect of low-dose cytarabine and aclarubicin in combination with gran-ulocyte colony-stimulating factor (G-CSF) protocol (CAG) in patients with acute myeloid leukemia (AML),and to understand the potential factors affecting the outcome of CAG induction therapy, therefore to find the optimum pa-tients for CAG therapy. Methods Twenty-one AML patients were enrolled in the current study. All patients were treated with CAG regimen including cytarabine (10 mg/m~2, subcutaneously, every 12 h, days 1 - 14), lacinomycin (5～7 mg/m~2,intravenously,every day, days 1 -8) ,and G-CSF (200 μg/m~2,subcutaneously, every day,12 h be-fore Ara-C was given) priming. Results The overall complete remission (CR) rate of the 21 AML patients was 66.7% (14/21). The CR rates was 87.5% (7/8) in patients older than 60 yrs,60.0% (9/15) in the refractory or relapsed patients,83.3% (5/6) in the MDS transformed AML patients. The CR rates for patients with hyperprolif-erative BM and median to poor proliferative BM were 33.3% and 91.7% ,respectively(P =0.009). The median o-verall survival (OS) time of the 21 AML patients was 450 days. Two-year survival rate estimated by Kaplan-Meier Method was 30.6%. The overall median disease free survival (DFS) was 165 days. The median OS time for those refractory or relapsed was 435 days. The median OS time for those with poor cytogenetic state or standard or good cytogenetic state was 140 days and 620 days, respectively (P = 0.001). The median OS time for patients with hyperproliferative BM and median to poor proliferative BM was 321 days and 620 days, respectively (P = 0.05). The median recovery time of granulocytes above 1.0×10~9/L was 8 days. The median duration of fever was 3.5 days. The rate of infections exceeding WHO grade Ⅱ was 42.9%. No early death occurred. Conclusions The CAG induction therapy may have a higher CR rate in patients with refractory or relapsed AML, elderly AML and secondary AML from MDS transformation, and extend the median overall survival time in refractory or relapsed patients. CAG therapy can not improve the outcome of patients whose BM was in high grade proliferation state or whose cytogenetic state was poor. CAG therapy can shorten the duration of agranulocytosis and decrease the inci-dence of serious infection. Therefore, CAG therapy is worth recommending to patients who can not endure the rou-tine intensive chemotherapy.

The tumor multidrug resistance reversal effect of NPB304, a novel taxane, was studied. MTT assay was used to determine the IC50 of chemotherapy drugs. Western blotting assay was applied to analyze the expression of P-glycoprotein (P-gp). The effect of compounds on the P-gp function and P-gp ATPase activity was determined by rhodamine 123 (Rh123) accumulation assay and analysis kit, respectively. Molecular docking was employed to predict the binding force between compounds and P-gp. Transmembrane transport of NPB304 was analyzed using MDCK II and MDR1-MDCK II cell model. NPB304 displayed multidrug resistance reversal effect on KBV cells and MCF-7/paclitaxel cells, NPB304 collaborative with P-glycoprotein (P-gp) inhibitors verapamil enhanced the reversal activity, specifically, 10 μmol x L(-1) verapamil in combination with paclitaxel reversed resistance by 56.5-fold, while combined with NPB304 increased the reversal fold; NPB304 synergistically increased Rh123 accumulation in the resistant cells when combined with verapamil, and NPB304 at 0-1 μmol x L(-1) enhanced the ATPase activity activated by verapamil was observed. NPB304 existed the hydrophobic interactions with the TM regions of P-gp, and the binding force between NPB304 and the A chain of the TM region was stronger. P-gp ATPase activity assay demonstrated NPB304 at lower concentrations (0-1.5 μmol x L(-1)) could activate the P-gp ATPase, playing a role on inhibition of P-gp function. However, NPB304 did not have an obvious feature of P-gp substrate. NPB304 exerted itself and synergy with verapamil activity on reversing tumor resistance via inhibiting the P-gp function.

Objective To investigate the influencing factors of survival rate in patients with myocardial infarction treated with intra-aortic balloon pump (IABP).Methods A total of 94 myocardial infarction patients with IABP in our hospital were selected,and were divided into survival group and death group according to the survival status.Clinical data,results of coronary angiography,emergency PCI results were compared,and the relevant influencing factors were analyzed by multivariate regression analysis.Results The average age,ratio of Killips Ⅲ/Ⅳ,CK-MB peak showed significant differences between two groups (P ＜ 0.05).Significant differences were seen in the proportion of patients merging LM disease and blood flow with class TIMI3 after PCI in the two groups (P ＜ 0.05).Multivariate regression analysis showed that the age,Killips Ⅲ/Ⅳ,CK-MB peak,blood flow with class TIMI3 after PCI,and merging LM lesions were the risk factors of survival status for patients.Conclusion The survival status of patients with IABP are mainly influenced by age,Killips Ⅲ/Ⅳ,CK-MB peak,blood flow with class TIMI3 after PCI,and merging LM lesions and other factors.

Objective To investigate the influencing factors of survival rate in patients with myocardial infarction treated with intra-aortic balloon pump (IABP).Methods A total of 94 myocardial infarction patients with IABP in our hospital were selected,and were divided into survival group and death group according to the survival status.Clinical data,results of coronary angiography,emergency PCI results were compared,and the relevant influencing factors were analyzed by multivariate regression analysis.Results The average age,ratio of Killips Ⅲ/Ⅳ,CK-MB peak showed significant differences between two groups (P ＜ 0.05).Significant differences were seen in the proportion of patients merging LM disease and blood flow with class TIMI3 after PCI in the two groups (P ＜ 0.05).Multivariate regression analysis showed that the age,Killips Ⅲ/Ⅳ,CK-MB peak,blood flow with class TIMI3 after PCI,and merging LM lesions were the risk factors of survival status for patients.Conclusion The survival status of patients with IABP are mainly influenced by age,Killips Ⅲ/Ⅳ,CK-MB peak,blood flow with class TIMI3 after PCI,and merging LM lesions and other factors.