Objective To establish the HPLC fingerprints of Rheum tanguticum from Qinghai Region.Methods By RP-HPLC,acetonitrile and water including 0.04% H_3PO_4 as mobile phase,gradient elution,flow rate was 1.0 mL/min,the detection wavelength was 280 nm,and the temperature was 40 ℃.Results The RSD of peak areas and retention time of common peak in the test of precision,repeatability,and stability has not reached 5%,respectively.Average similarity was 0.925 in R.tanguticum collected from different habitats.Conclusion All above results show that this method is simple,practicable,and reliable.It can be used as a standard analysis method in the quality control of R.tanguticum from Guoluo Region in Qinghai Province.It also provides a theory foundation on which the species cultivated could replace the wild ones.

Objective To characterize the clinic features of fulminant type 1 diabetes mellitus (IT1DM).Methods It was a retrospective study analyzing the clinical and laboratory data of patients diagnosed as FT1DM and type 1 diabetes in the Department of Endocrinology in China-Japan Friendship Hospital from January 2011 to November 2015.Results A total of 125 newly diagnosed type 1 diabetic patients were included in the study,14 of them (11.2％) met with the criteria of FT1DM.The age at onset was (35.8 ± 8.3) years.The time from onset to ketosis was 2 (0-7) days.The plasma glucose levels were extremely high[(33.79 ± 14.13)mmol/L],while glycosylated hemoglobin A1c[HbA1c,(6.9 ±0.7) ％] and serum glycosylated albumin [(21.8 ± 4.5)％] levels were only slightly above the normal range.Moreover,the C-peptide levels were extremely low and the situation kept after one month to two years' follow-up.Four (28％) patients were glutamic acid decarboxylase antibody (GADA) positive at the onset with two turned negative.One patient was GADA negative at the onset and turned positive after one month.The levels of serum transaminases increased with enlargement of liver in one of the patients after insulin therapy,who was diagnosed as glycogenic hepatopathy.Conclusions FT1 DM is an extremely rapidly progressing type of diabetes and life threatening disease.Causations have to be taken in medical practice.

Objective: To investigate the relationship between undergraduates’ emotional intelligence, personality traits and self-handicapping. Methods: 1094 undergraduates were tested with Self-handicapping Questionnaires、Emotional Intelligence Scale and NEO-FFI. Results: ①Undergraduates’ self-handicapping was negatively correlated with emotional intelligence(P

Objective To study psychological change on anxiety patients with coronary heart disease (CHD) after percutaneous coronary intervention and to explore the effect of psychological intervention on anxiety patients with CHD.Methods The selected 126 anxiety patients with CHD were randomly divided into three groups A,B,C,and 42 cases in each group.Group A of was the control group,group B was the anti-anxiety drug group,group C was the psychological intervention combinded with anti-anxiety drug,and all patients were treated for one month.The self-rating anxiety scale (SAS) scores were recorded in three groups before surgery,after one week and one month treatment.Resluts There was no significant difference in SAS scores in group A before the operation and after 1 week,1 month treatment(respectively(57.14 ±5.63),(55.86 ±5,46),(53.93 ±6.03),P＞ 0.05).After 1 week and 1 month treatment,the SAS score of group C (respectively (42.79 ± 5.82) points,(33.86 ± 5.05) points) and the SAS score of group B (respectively (49.93 ± 6.63) points,(40.93 ± 6.46)points) were lower than those of group A (respectively (55.86 ± 5.46) points,(53.93 ± 6.03) points),and there were significant differences among the three groups(P ＜ 0.05).Conclusion There is no significantly improvement in patient's anxiety after coronary revascularization.Anti-anxiety drug and psychological intervention significantly improve the patient's negative emotions and reduce the incidence of angina.

Objective To investigate the effects of Lactobacillus acidophilus tablets combined with infantile diarrhea paste in the treatment of acute diarrhea in children.Methods Retrospective study of a total of 92 children with acute diarrhea from our hospital were collected and randomly divided into experimental group and control group with 46 cases in each group.Patients in the control group were treated by conventional treatment, patients in the experimental group were treated by Lactobacillus acidophilus tablets combined with infantile diarrhea paste.The serum myocardial enzyme, isozyme, inflammatory factors and the levels of T lymphocyte were determined, and the clinical efficacy before and after treatment was compared.Results The clinical effective rate of the control group ( 82.61%) was lower than the experimental group ( 95.65%) , with statistical significance ( P<0.05 );compared with the control group, after treatment the serum myocardial enzyme and isozyme of the experimental group decreased, after treatment the serum TNF-α, IL -6 and I -10 levels decreased, after treatment the CD3 +, CD4 + and CD4 +/CD8 +levels increased, CD8 + level decreased, with statistical significance ( P<0.05 ) , There was no adverse reactions such as gastrointestinal discomfort and skin irritation happened in two groups of patients.Conclusion Lactobacillus acidophilus tablets combined with infantile diarrhea paste in the treatment of children with acute diarrhea could reduce serum myocardial enzymes and isozyme levels, improve immune ability and the effect is remarkable.

Objective To discuss the clinical features and treatment of hepatogenous diabetes. Method Retrospective analysis was conducted in January 2002-April 2009 170 cases of clinical data in patients with liver cir-rhosis. Result 170 cases of liver cirrhosis 40 cases of diabetes, the incidence was 23.5% ,various types of cirrhosis may occur;typical "over three" obvious symptoms; 10 cases admitted tohospital when fasting blood glucose≥7.0 mmol/L, postprandial 2 hours blood glucose were ≥1.1 mmol/L;high level of total bilirubin,serum albumin,cholin-esterase,total cholesterol low. Given to diabetic diet and small doses of ct-glucosidase, short-acting insulin treatment; 26 cases of normal blood glucose, improvement in 8 cases (blood glucose 6. 1～11.1 mmol/L) ;6 deaths. Conclusion Prone to occur in patients with liver cirrhosis glucose metabolism disorders, its clinical features, treatment of liver damage and the need to take into account two aspects of diabetes, the use of insulin as the main.

AIM To investigate the effects of taurine(TAU) and propafenone(PRO) combination on experimental arrhythmias and its influence on haemodynamics of anesthesized rats' heart. METHODS Rats were pretreated respectively with intravenous administration of saline, TAU, PRO, or TAU and PRO combination. Afterwards, the rats were intravenously administrated with BaCl 2 or aconitine to induce arrhythmia. The arrhythmia lasting time and incidence were observed and recorded on the lead Ⅱ ECG. The cumulative aconitine dosage to induce premature ventricular contraction(PVC), ventricular tachycardia(VT) and ventricular fibrillation(VF) were calculated respectively. The haemodynamic parameters such as LVSP, HR, +d p /d t max were recorded continuously in anesthetized rats pretreated with iv TAU, PRO or TAU and PRO combination. RESULT In BaCl 2 arrhythmia, the arrhythmia duration was shortened significantly; and the arrhythmia incidence was decreased in the combination group, as compared with the single same dosage drug groups. In aconitine arrhythmia, the cumulative aconitine dosages to induce PVC, VT and VF were increased significantly in the combination group. Haemodynamics parameters showed that PRO(2mg?kg -1 iv) significantly decreased LVSP and +LVd p /d t max , while these parameters were not significantly changed in the combination group with same dose of PRO. CONCLUSION TAU and PRO have synergism in antiarrhythmia in both BaCl 2 and aconitine induced arrhythmia; TAU may alleviate cardiac depression exerted by PRO.

Objective To analyze the correlation between fetal right aortic arch and chromosome ab-normality by ultrasound.Methods From January Jan 2009 to Dec 2014,nineteen cases with fetal right aortic arch were enrolled.They were all determined by chromosome karyotype analysis.The correlation of fetal right aortic arch and chromosome abnormalities detected by ultrasound were evaluated.Results Left lock,a “U”shaped vascular structure,were found in 15 cases.One case of fetal aortic arch was surrounded by a “O”shaped package.Three cases of right aortic arch showed fetal left artery catheter and brachiocephalic artery ima-ging branch.Echocardiography mainly for arterial catheter in three vessel trachea view did not show up.Artery catheter,in front of the trachea,did not form the vascular ring.Nineteen cases of right aortic arch were exam-ined by fetal karyotype analysis.Three patients with trisomy 18-karyotype presented ventricular septal defect, single artium,three tricuspid atresia,pulmonary artery stenosis.Four cases with trisomy 18-karyotype presen-ted ventricular septal defect,complete atrioventricular canal,single artium,double outlet right ventricle,pul-monary artery stenosis in atresia.Three cases with trisomy 21 -karyotype showed single ventricle,single atri-um.One case was with tetralogy of Fallot and one case with dextrocardia,aortic stenosis,accompanied by 22q 1 1.2.Conclusion In prenatal ultrasound screening,we should pay attention to three vessels-trachea view on ultrasound image,which can improve the detection rate of right aortic arch.Fetal right aortic arch and triso-my 18-,trisomy 21 -,chromosomal diseases are substantially correlated.Further analysis of chromosome kary-otype is needed to exclude chromosomal lesions,so as to achieve the purpose of prenatal and postnatal care.

0.05). The BHR distribution was slight 31.9%, mild 43.5%, moderate 24.6%, no severe BHR were found in this study, showing less correlation between the percentage of predicted FEV 1 and the PD 20FEV 1-His in the elderly (r=0.277, P