Objective To establish the HPLC fingerprints of Rheum tanguticum from Qinghai Region.Methods By RP-HPLC,acetonitrile and water including 0.04% H_3PO_4 as mobile phase,gradient elution,flow rate was 1.0 mL/min,the detection wavelength was 280 nm,and the temperature was 40 ℃.Results The RSD of peak areas and retention time of common peak in the test of precision,repeatability,and stability has not reached 5%,respectively.Average similarity was 0.925 in R.tanguticum collected from different habitats.Conclusion All above results show that this method is simple,practicable,and reliable.It can be used as a standard analysis method in the quality control of R.tanguticum from Guoluo Region in Qinghai Province.It also provides a theory foundation on which the species cultivated could replace the wild ones.

Objective To study psychological change on anxiety patients with coronary heart disease (CHD) after percutaneous coronary intervention and to explore the effect of psychological intervention on anxiety patients with CHD.Methods The selected 126 anxiety patients with CHD were randomly divided into three groups A,B,C,and 42 cases in each group.Group A of was the control group,group B was the anti-anxiety drug group,group C was the psychological intervention combinded with anti-anxiety drug,and all patients were treated for one month.The self-rating anxiety scale (SAS) scores were recorded in three groups before surgery,after one week and one month treatment.Resluts There was no significant difference in SAS scores in group A before the operation and after 1 week,1 month treatment(respectively(57.14 ±5.63),(55.86 ±5,46),(53.93 ±6.03),P＞ 0.05).After 1 week and 1 month treatment,the SAS score of group C (respectively (42.79 ± 5.82) points,(33.86 ± 5.05) points) and the SAS score of group B (respectively (49.93 ± 6.63) points,(40.93 ± 6.46)points) were lower than those of group A (respectively (55.86 ± 5.46) points,(53.93 ± 6.03) points),and there were significant differences among the three groups(P ＜ 0.05).Conclusion There is no significantly improvement in patient's anxiety after coronary revascularization.Anti-anxiety drug and psychological intervention significantly improve the patient's negative emotions and reduce the incidence of angina.

Objective To characterize the clinic features of fulminant type 1 diabetes mellitus (IT1DM).Methods It was a retrospective study analyzing the clinical and laboratory data of patients diagnosed as FT1DM and type 1 diabetes in the Department of Endocrinology in China-Japan Friendship Hospital from January 2011 to November 2015.Results A total of 125 newly diagnosed type 1 diabetic patients were included in the study,14 of them (11.2％) met with the criteria of FT1DM.The age at onset was (35.8 ± 8.3) years.The time from onset to ketosis was 2 (0-7) days.The plasma glucose levels were extremely high[(33.79 ± 14.13)mmol/L],while glycosylated hemoglobin A1c[HbA1c,(6.9 ±0.7) ％] and serum glycosylated albumin [(21.8 ± 4.5)％] levels were only slightly above the normal range.Moreover,the C-peptide levels were extremely low and the situation kept after one month to two years' follow-up.Four (28％) patients were glutamic acid decarboxylase antibody (GADA) positive at the onset with two turned negative.One patient was GADA negative at the onset and turned positive after one month.The levels of serum transaminases increased with enlargement of liver in one of the patients after insulin therapy,who was diagnosed as glycogenic hepatopathy.Conclusions FT1 DM is an extremely rapidly progressing type of diabetes and life threatening disease.Causations have to be taken in medical practice.

Objective: To investigate the relationship between undergraduates’ emotional intelligence, personality traits and self-handicapping. Methods: 1094 undergraduates were tested with Self-handicapping Questionnaires、Emotional Intelligence Scale and NEO-FFI. Results: ①Undergraduates’ self-handicapping was negatively correlated with emotional intelligence(P

Objective To investigate the effects of Lactobacillus acidophilus tablets combined with infantile diarrhea paste in the treatment of acute diarrhea in children.Methods Retrospective study of a total of 92 children with acute diarrhea from our hospital were collected and randomly divided into experimental group and control group with 46 cases in each group.Patients in the control group were treated by conventional treatment, patients in the experimental group were treated by Lactobacillus acidophilus tablets combined with infantile diarrhea paste.The serum myocardial enzyme, isozyme, inflammatory factors and the levels of T lymphocyte were determined, and the clinical efficacy before and after treatment was compared.Results The clinical effective rate of the control group ( 82.61%) was lower than the experimental group ( 95.65%) , with statistical significance ( P<0.05 );compared with the control group, after treatment the serum myocardial enzyme and isozyme of the experimental group decreased, after treatment the serum TNF-α, IL -6 and I -10 levels decreased, after treatment the CD3 +, CD4 + and CD4 +/CD8 +levels increased, CD8 + level decreased, with statistical significance ( P<0.05 ) , There was no adverse reactions such as gastrointestinal discomfort and skin irritation happened in two groups of patients.Conclusion Lactobacillus acidophilus tablets combined with infantile diarrhea paste in the treatment of children with acute diarrhea could reduce serum myocardial enzymes and isozyme levels, improve immune ability and the effect is remarkable.

Objective To investigate the incidence of pain for the.elderly with dementia in our country.Methods The Chinese version of Doloplus-2 was used to assess pain in 103 patients with dementia.Results One or more specific diseases may cause pain in 94 patients,degeneration of bone and joint top of the list;69 patients with pain score geater than or equal to 5 points,the incidence of pain as much as 67% .Older persons in different age groups the incidence of pain is different,with the age increased the incidence of pain increased;different older age groups pain scores had no significant difference.Conclusion Pain in patients with dementia should be of great importance to the medical staff and give full play to the nursing staff in pain management.

Objective To explore more effective treatments for irritable bowel syndrome(IBS)in old people.Methods 80 patients with irritable bowel syndrome were selected.adverse drug reaction during treatment,and therapy effect of a symptom score after treatment observation group and control group were compared.Results Total improvement rate of symptoms from observation group was 91.7％,and total improvement rate of symptoms in control group was 65.6％.Difference of proportions of significant improvement and improvement was significant,according to the situation that was controlled between observation group and control group.The difference of symptom score after treatment was significant,according to the situation that was controlled between observation group and control group.Conclusion Trimebutine maleate,oryzanol and anisodamine combined application could remarkably improve irritable bowel syndrome treatment effect,and was more safe.

ObjectiveTo explore curative effect of eradication of Helicobacter pylori by sequential therapy and traditional triple therapy.MethodsTo retrospective analyze clinical data of Helicobacter pylori masccline patients,which were divided into the sequential therapy group and the traditional triple therapy group.ResultsThe results of ITT analyze and PP analyze of the sequential therapy group were higher than those of the traditional triple therapy group (x2 =19.30,11.18,all P ＜ 0.05) ;The total effective rate of clinical curing of the sequential therapy group was higher than that of the traditional triple therapy group( x2 =14.25,P ＜0.05 ) ;The untoward reaction rate of the sequential therapy group was lower than that of the traditional triple therapy group( x2 =10.53,6.93,6.93,all P ＜0.05 ).ConclusionThe curative effect of eradication of Helicobacter pylori by the sequential therapy were obviously and little untoward reaction rate,which was to be worthy used.

Objective To analyze the methylation status of Vimentin gene in feces of colorectat cancer (CRC) patients,to explore the possibility and clinical significance of which as molecular marker for early diagnosis of colorectal cancer.Methods DNA was isolated in feces of 60 colorectal cancer patients hospitalized and treated in the First Affiliated Hospital of Zhengzhou University,17 colorectal adenoma patients underwent colon endoscopy in outpatient department and 30 normal volunteers.The methylation status of Vimentin gene was analyzed with methylation-specific PCR (MSP).Results In the feces of 60 colorectal cancer patients,the positive percentage of Vimentin gene methylation was 51.67％ (31/60).In the feces of 17 colorectal adenoma patients,the positive percentage of Vimentin gene methylation was 4/17.Vimentin gene methylation was detected in none of 30 normal controls.Conclusion The methylation of Vimentin gene in fecal is an early event in colorectal cancer progression.Analysis of Vimentin gene methylation in feces is expected to be one of the markers for early diagnosis of colorectal cancer.

Objective: To study the quality standards for Shenyanghongyao Tablets. Methods: Radix Angelicae Dahuricae was identified by TLC and Ginsenoside R g1 in Radix Notoginseng was determined by TLCS. Results: The linear relationship was at the range of 0.92?g～4.60?g. The average recovery was 97.16%. Conclusion: The methods are available with a good reproducibility and can control the quality of this preparation effectively.