Objective To evaluate a new gene therapy for the treatment of experimental occlusive arterial disease Methods Magnetic nanospheres were produced, VEGF gene was cloned for subsequent construction of eukaryotic expression plasmid The magnetic gelatin microspheres used in targeted gene therapy were prepared by emulsion crosslinking method The microspheres were injected intrafemorally in rabbits through contralateral femoral artery, and the ischemic limb was placed in a magnetic field Angiography was performed on day 10 and day 30 respectively The capillary density and the capillary to muscle fiber ratio were determined histochemically Results Compared to the controls there was significant collateral artery development in VEGF transfected group The capillary density and the capillary to muscle fiber ratio were significantly higher for the VEGF transfected group than for the control group. The capillary density of control was (125?23)/mm 2, and in VEGF group was (298?27)/mm 2, P

Magnesium based implants have the characteristics of bio-degradability, osteoconductive, and, regulatory strength. After the tissue has healed sufficiently, the burden of a second surgical procedure can be avoided. However, the degradation speed is so fast as to limit its clinical application. Hence, it is crucial for the biomedical magnesium alloys to be able to change their biodegradation behavior and speed. This paper reviews the degradability, biological activity and biocompatibility of magnesium and its alloys as orthopedic biomaterial in vitro and vivo to explore the possible way to modify the characteristics of its degradability, for the purpose of controllable degradation speed.
Absorbable Implants ; Alloys ; chemistry ; Animals ; Biocompatible Materials ; chemical synthesis ; Bone Plates ; Bone Screws ; Bone Substitutes ; chemistry ; Humans ; Magnesium ; chemistry

Objective To improve clinical technique of nursing students by introducing multimedia teaching software of digital intravenous infusion technology into teaching process. Methods A total of 50 nursing students who were chosen by convenient sampling in the First Affiliated Hospital of Harbin Medical University from May 2016 to July 2016 were divided into observation group and control group by lottery method. Students in control group received traditional teaching method, while observation group studied multimedia intravenous infusion technology under the guidance of teachers. Successful rate of first puncture, single operation time, related knowledge as well as patients' pain were compared between the two groups. Results Success rate of venipuncture of the observation group was higher than that of the control [91% vs. 81%; χ2=7.72;P=0.009]. Single operation time, score of related knowledge of the observation group were (3.10±0.88)s, (96.40±1.71) points compared to those of the control group(4.60±1.07)s,(92.20±2.74)points(t=-3.42, 4.11;P<0.01). Score of patients' pain of the observation group was less than that of the control group[(3.30±0.82) points vs.(4.30±1.06)points; t=-2.36;P=0.030]. Conclusions The development and application of the software makes the simulation operation closer to clinical practice for nursing students.