1.The effect of sodium butyrate in combination with ATRA on the proliferation/differentiation of SKM-1.
Mei HUANG ; Wenli LIU ; Chunrui LI ; Jinniu DENG ; Jianfeng ZHOU ; Donghua ZHANG ; Hanying SUN
Journal of Huazhong University of Science and Technology (Medical Sciences) 2004;24(4):334-337
To explore the molecular mechanisms of sodium butyrate working on SKM-1 cell proliferation/differentiation and to study its synergistic effect with all-trans retinoic acid (ATRA), SKM-1 cells were grown in the absence or presence of sodium butyrate and/or ATRA. The percentage of viable cells was determined by trypan blue exclusion. Differentiation was determined by nitroblue tetrazolium (NBT) reduction and cell surface adhesion molecules was analyzed by FACS. Cell cycle distribution was examined after DNA staining by propidium iodide. D-type cyclins, cdks and P21 mRNA were studied by reverse transcription-polymerase chain reaction. Our results showed that sodiun butyrate and/or ATRA blocked cells mainly in the G0/G1 phase of the cell cycle. ATRA inhibited the mRNA expression of CDK6, CDK4, cyclinD3 and cyclinD1. Sodium butyrate inhibited the mRNA expression of CDK2, cyclinD2 and cyclinD1. ATRA and sodium butyrate inhibited the mRNA expression of CDK6, CDK4, CDK2, cyclinD1, cyclinD2 and cyclinD3. Both ATRA and/or sodium butyrate stimulated p21 expression at the mRNA levels. Our results suggest that the effect of sodium butyrate on cell proliferation/differentiation might be linked to its ability to induce expression of p21 mRNA and inhibit the cyclin-cdk complexes. Our observations support the notion that the sodium butyrate works synergistically with ATRA.
Antineoplastic Agents
;
pharmacology
;
Butyrates
;
pharmacology
;
Cell Cycle Proteins
;
biosynthesis
;
genetics
;
Cell Differentiation
;
drug effects
;
Cell Division
;
drug effects
;
Cyclin-Dependent Kinase Inhibitor p21
;
Drug Interactions
;
Humans
;
Leukemia, Monocytic, Acute
;
pathology
;
RNA, Messenger
;
biosynthesis
;
genetics
;
Tretinoin
;
pharmacology
;
Tumor Cells, Cultured
2.Retrospective analysis of the risk factors for secondary pulmonary infection after cervical spinal cord injury
Qianqian JIN ; Jianxian WU ; Jinniu ZHANG ; Juehua JING ; Dasheng TIAN ; Xiaojun FENG ; Yongfeng HONG ; Yun ZHOU
Chinese Journal of Physical Medicine and Rehabilitation 2021;43(11):983-986
Objective:To analyze retrospectively the risk factors for pulmonary infection after traumatic cervical spinal cord injury.Methods:The 154 patients with a cervical spinal cord injury studied included 120 with a pulmonary infection and 34 uninfected controls. Regressions were evaluated using data on their genders, ages, the cause of injury, affected segments, the neurological level of the injury (NLI), and the presence of a vertebral fracture or dislocation.Results:Age, complete injury, NLI at C 1 to C 4, and an injury-to-treatment time of more than 8 hours were found to be independent risk factors for secondary pulmonary infection. Conclusion:Elderly spinal cord injury patients, with a complete injury, an NLI between C 1 and C 4 or an injury-to-treatment time of more than 8 hours have a higher risk of pulmonary infection.
3.A case of type 3 progressive familial intrahepatic cholestasis
Tianying ZHANG ; Chao ZHOU ; Weihui LIU ; Zhenmao LI ; Liangping LI ; Lei LEI
Journal of Clinical Hepatology 2022;38(11):2568-2570
4.Effect of early individualized rehabilitation on patients with severe mechanical ventilation
Jing MAO ; Xiaoxiao TANG ; Yao ZHENG ; Jinniu ZHANG ; Xiuli KAN ; Jianxian WU
Chinese Journal of Rehabilitation Theory and Practice 2022;28(6):710-715
ObjectiveTo explore the effect of early individualized rehabilitation on patients with severe mechanical ventilation. MethodsA total of 36 patients on mechanical ventilation admitted to the ICU of the Second Affiliated Hospital of Anhui Medical University from March, 2019 to February, 2020 were randomly divided into control group (n = 18) and rehabilitation group (n = 18). All the patients completed a rehabilitation assessment within 24 hours of admission, including clinical assessment, state of consciousness and muscle strength assessment. The control group was treated with intensive care routine treatment, including symptomatic treatment of primary disease, nutritional support, placement of normal limbs, active and passive movement of limbs. The rehabilitation group received early individualized rehabilitation in addition., including active and passive limb movements, transfer training, physical factor therapy, and respiratory muscle training after the specific evaluation. The mechanical ventilation duration and ICU length of stay, the hospitalization cost, Richmond Agitation and Sedation Scale (RASS), acute Physiology and Chronic Health Evaluation Ⅱ (APACHEⅡ), and the content of tumor necrosis factor (TNF)-α, interleukin (IL)-6 and IL-8 were compared. ResultsThere was no significant difference in the weaning rate and hospitalization cost between two groups (P > 0.05). The mechanical ventilation duration and ICU length of stay were less in the rehabilitation group than in the control group (t > 2.067, P < 0.05). After treatment, the score of APACHEⅡ and the content of TNF-α and IL-6 decreased in the control group (t > 2.040, P < 0.05); the score of APACHEⅡ and the content of TNF-α, IL-6 and IL-8 decreased in the rehabilitation group (t > 4.141, P < 0.001); the content of TNF-α, IL-6 and IL-8 was less in the rehabilitation group than in the control group (t > 2.217, P < 0.05). The improvement of all the indexes was better in the rehabilitation group than in the control group (|Z| > 2.104, P < 0.05). ConclusionFor patients on mechanical ventilation, early individualized rehabilitation could improve the sedation, relieve the inflammatory reaction, accelerate the process of weaning, and reduce the length of stay with no extra cost.
5.Multicenter study on the effect of early screening skills training for autism spectrum disorders in primary care hospitals in Chengdu
Wenxu YANG ; Jiao LE ; Lan ZHANG ; Ying ZHANG ; Ping YANG ; Chunxia ZHAO ; Chunhua DU ; Junni HE ; Yanmei CAO ; Jia SHANG ; Li LI ; Yan LIU ; Shenglan WU ; Xia LI ; Xiujin CHEN ; Hai LAN ; Hua LI ; Xiang KONG ; Hengli LI ; Defang MI ; Jie ZHAO ; Yang NIE ; Jinxiu GAO ; Ling LI
Sichuan Mental Health 2022;35(4):337-342
ObjectiveTo investigate effect of conducting training of autism spectrum disorder (ASD) early screening skill on improving the ability to early identify ASD of medical staffs in primary care hospitals. MethodsIn September 2021, the training of ASD early screening skills was carried out for medical staffs from 20 primary care hospitals in Chengdu. After training, the training effect was evaluated. The numbers of referrals from primary care hospitals to superior hospitals, confirmed ASD as well as their average diagnostic age of children with ASD before and after training were used as evaluation indicators. ResultsAfter training, the number of children with suspected ASD referred by primary care hospitals was more than that before training [(16.65±11.60) vs. (3.40±2.23), t=5.431, P<0.01], the number of children diagnosed with ASD was more than that before training[(6.85±4.93) vs. (2.45±1.67), t=4.171, P<0.01], and the differences were statistically significant. As for the diagnosed age of ASD children, after training, the average age was lower than that before training [(34.95±11.67) vs. (42.2±14.64), t=-2.553, P=0.019]. ConclusionTraining of ASD early screening skills for medical staffs in primary care hospitals may help to improve their ability to early screening ASD children.