Objective To make students at different levels not only learn theoretical knowledge of acupuncture-moxibustion manipulation skills but also achieve full operating training, strengthen the combination of theory and practice and improve the teaching quality of clinical disciplines by applyingmultimedia-subject participationteaching method to acupuncture-moxibustion manipulation techniques in the experimental teaching of acupuncture and moxibustion. Method The students were randomized, according to grade, into experiment (n=114) and control (n=115) groups. The teaching content was the same in the two groups. The experiment group received multimedia-subject participationteaching method. After the multimedia teaching, the students did an independent demonstration and made a self-evaluation, the teachers made a comment and the students did independent practice again in groups. The control group received conventional theory teaching and did practice under demonstration in the experimental lesson. The examination and questionnaire were used as assessment indicators to evaluate the effect ofmultimedia-subject participationteaching method applied to acupuncture-moxibustion technique teaching. Result Statistical analysis was made using the SPSS11.5 statistical software package. The independent samples t-test is used for a between-groups comparison. The statistical result showed that satisfaction was 100%in the experiment group and 75.7%in the control group;there was a statistically significant difference between the two groups (P<0.05). Conclusion Multimedia-subject participationteaching method is an innovation in acupuncture-moxibustion technique teaching and helps the students to better master acupuncture-moxibustion manipulation skills. Mastery of theoretical knowledge before real practice is the key to proficient manipulation. The combination of the two in classroom teaching yields twice the result with half the effort. Carrying out multimedia-subject participation teaching method will help to further deepen the teaching reform and to improve the level and quality of“Acupuncture and Moxibustion”teaching.

Objective:To screen and identify H-2 d-restricted T cell epitopes in fusion (F) and attachment (G) glycoproteins of Nipah virus (NiV) in mice. Methods:The complete peptides (single peptide contains 15 amino acids, and 10 amino acids were repeated in the front and back peptides) derived from F and G antigens were mixed into peptide libraries. BALB/c mice were immunized with DNA vaccines expressing NiV F and G proteins alone and in combination. The full sequence peptide libraries of F and G antigens were mixed into peptide pools by matrix design, and spleen cells of immunized mice were collected and analyzed by IFN-γ ELISPOT assay to detect the dominant H-2 d-restricted epitope peptides. Results:Twelve dominant H-2 d-restricted peptides were screened from the F protein-specific peptide library and the 56th peptide produced the strongest reaction. Four dominant peptides were screened from the G protein-specific peptide library and the 72nd peptide produced the strongest reaction. Conclusions:In this study, 12 F antigen-specific and 4 G antigen-specific H-2 d restricted dominant T cell epitopes of NiV were screened and identified by IFN-γ ELISPOT, which could provide reference for immunological analysis of NiV and vaccine research.

Liver cholesterol metabolism disorder plays an important role in the development of non-alcoholic fatty liver disease (NAFLD).In order to reveal the molecular mechanism of cholesterol homeostasis imbalance induced by saturated fatty acids, HepG2 cells were stimulated with palmitic acid (PA).Lipids accumulation was analyzed by Oil Red O staining, intracellular triglyceride and cholesterol quantification.The level of genes and proteins related to cholesterol homeostasis was measured by RT-qPCR and western blotting.Additionally, intracellular bile acids and mitochondrial oxysterols were detected by LC-MS/MS.The results demonstrated that intracellular lipids such as TG and TC were significantly increased in the model with PA stimulation.Although no significant difference was detected in genes related to cholesterol synthesis and uptake, the protein expression of ABCG5 and LXRα were significantly down-regulated, indicating a decrease in cholesterol efflux.Meanwhile, the gene expression of STARD1 and CYP7B1, which are responsible for bile acid alternative synthesis, were markedly enhanced, along with a significant increase of cholesterol and 27-OHC in mitochondria and CDCA in cells.These results suggested that PA overload may disrupt cholesterol homeostasis by inhibiting cholesterol efflux and promoting bile acids synthesis.

Objective : To investigate the protective effect of wogonin on acute kidney injury (AKI) induced by li- popolysaccharide (LPS) ． Methods : The model of septic-induced AKI was established on male C57BL /6J mice by a single intraperitoneal injection of LPS and normal C57BL /6J mice were used as normal control group．Twenty-four male C57BL /6 mice were randomly divided into 4 groups (6 mice in each group) : normal control group ( NC) ， normal control + wogonin (NC + WOG 12. 5 mg / kg) ，LPS model group ( LPS 10 mg / kg) ，LPS model + wogonin (LPS 10 mg / kg + WOG 12. 5 mg / kg) ．After LPS intervention for 24 h ，serum samples were collected to detect blood creatinine ( CRE) and urea nitrogen (BUN) levels．HE staining and PAS staining were performed to observe the degree of renal pathological injury．Immunohistochemistry was performed to detect the degree of expression of inflammatory markers interleukin ( IL) -1 β , IL-6 and tumor necrosis factor ( TNF) -α in renal tissues． PCR was performed to detect the expression of KIM-1 and NGAL in renal tissues．Western blot was performed to detect the changes in protein expression of NF-κB signaling pathway subunits P65 and PP65 in renal tissues. Results : Com- pared with NC group，CRE and BUN levels in LPS group increased (FCRE = 60. 90，P＜0. 001，FBUN = 82. 13，P ＜0. 001) ; compared with LPS group，these indexes decreased in LPS + WOG group (P＜0. 001) ．PCR test re- sults showed that compared with the NC group ，the expression of KIM-1 and NGAL mRNA was significantly increased in LPS group(FKIM-1 = 146. 3，P＜0. 001，FNGAL = 161. 2，P＜0. 001) ．In contrast，KIM-1 and NGAL mR- NA expression was decreased in the LPS + WOG group (P ＜0. 01) . Renal histopathological examination showed that compared with the NC group，renal tissues of mice had renal tubular dilatation and inflammatory cell infiltration in LPS group ; compared with LPS group，the number of tubular dilatation reduced and inflammatory cell infiltration was reduced in LPS + WOG group ( FHE = 721. 4，P ＜0. 001 ; FPAS = 518. 9，P ＜0. 001) ．Immunohistochemical staining showed that the expression of IL-1 β , IL-6 and TNF-α was significantly increased in the LPS group ; com- pared with the LPS groups ( FIL-1β = 114. 6 ，FIL-6 = 108. 9 ，FTNF-α = 251. 6 ，all P ＜0. 001 ) ，these indexes decreased in LPS + WOG group (all P＜0. 01) ．Further studies using Western blot showed that the NF-κB signaling pathway of LPS-treated mice had been activated and produced a hyperphosphorylated state in comparison to the NC group (FPP65 = 13. 02，P＜0. 01) ，yet this pathway in the LPS + WOG group showed the opposite effect，namely attenuated activity and reduced phosphorylation when the control was LPS (P＜0. 01) ． Conclusion WOG effectively blocked the NF-κB signaling pathway in the LPS-induced acute kidney injury model mice，thereby attenua- ting the inflammatory response and tissue damage in the kidneys of LPS-induced acute kidney injury mice.