Objective To generally analyse the current situations and advances in the biotherapy of gastric cancer. Methods The published papers about the current situations and research advances in the biotherapy of gastric cancer were reviewed.Results The main research fields and treatments in the biotherapy of gastric cancer include immunomodulator therapy, guided therapy of monoclonal antibodies and their joint-bodies, cytokine therapy, adoptive immunotherapy and gene therapy, etc. Conclusion As a beneficial complement for surgery, chemotherapy and radiotherapy, biotherapy plays an important auxiliary role in the multiple treatments for gastric cancer.

As a guanine nucleotide dissociation stimulator (GDS) of Rho-like GTPases, T lymphoma invasion and metastasis inducing factor 1(Tiam 1)possesses such biological functions as regulating reconstruction of cytoskeletal structure and migratory potential of cells. Studies had shown that over-expression of Tiam 1 was able to induce invasion and metastasis of tumor cells, the molecular biological basis of which includes the interactions between Tiam 1 and trans-membrane system of cytoskeleton, adhesion molecules and extra-cellular matrix, the effect on proliferation and apoptosis of tumor cells exposed by Tiam 1, as well as the activities of Tiam 1 regulated by other invasion and meatstasis associated factors. Simultaneously, these experimental results suggested that much more work would be needed in Tiam 1 on the diversity of its activity regulation ,the specificity of its biological effect, the realationship among Tiam 1 with other invasion and metastasis associated factors, as well as their mechanisms of signal transduction.

Mitochondrial fatty acids ?-oxidation is a repetitive process of four steps which provides the major source of energy for heart, liver and skeletal muscle. Several enzymes are involved in this spiral cycle. The medium-chain acyl-CoA dehydrogenase ( MCAD) , the short-chain acyl-CoA dehydrogenase (SCAD) , the long-chain 3-hydroxy acyl-CoA dehydrogenase (LCHAD) and the carnitine-palmitoyl-CoA transferase Ⅱ ( CPT Ⅱ ) deficiency have been recognized as the most common inborn errors of metabolism and frequently reported in their association with sudden infant death ( SID) . The prevalent mutations in these genes need further investigation in different populations.

Objective To investigate the expression of heat shock protein 70 (HSP70) in maternal serum,umbilical cord blood and placenta of patients with hypertensive disorders complicating pregnancy (HDCP) and to discuss its role in the pathogenesis of HDCP.Methods Totally 90 patients with HDCP were recruited as HDCP group,and were devided into three subgroups,including gestational hypertension group (30 cases),mild preeclampsia group(30 cases) and severe preeclampsia group(30 cases).A totally of 30 cases of healthy pregnant women were defined as the control group.All of them were admitted to Xuzhou Maternity and Child Health Care Hospital from August 2011 to December 2012.ELISA was used to detect the expression of HSP70 in maternal serum and umbilical cord blood.Immunohistochemistry streptavidin peroxidase(SP) was used to detect the protein in placenta,and semi-quantitative reverse transcription (RT)-PCR was used to detect the expression of HSP70 mRNA.Results (1) The levels of HSP70 in maternal serum and cord blood of mild preeclampsia group were (2.61±0.98) and (0.78±0.27)μg/L,respectively; and were (3.10± 1.18) and (0.96±0.28)μg/L in severe preeclampsia group.The levels of HSP70 in mild and severe preeclampsia groups were significantly higher than those in the control group [(1.88±0.79) and (0.61±0.15) μg/L,respectively] and gestational hypertension group [(2.13 ± 0.71) and (0.64 ± 0.18) μg/L,respectively;P＜0.05].The level of HSP70 in severe preeclampsia group was significantly higher than that in mild preeclampsia group (P＜0.05).And the level of HSP70 in gestational hypertension group was higher than that in the control group,but there was no statistical difference (P＞0.05).(2)The positive rate of placental HSP70 in gestational hypertension group,mild and severe preeclampsia group [83％(25/30),90％ (27/30) and 100％(30/30)],respectively were significantly higher than those in the control group(43％,13/30;P＜0.05).The positive rate of placental HSP70 in severe preeclampsia group was significantly higher than that in gestational hypertension group and mild preeclampsia group(P＜0.05).(3)The expression of placental HSP70 mRNA in gestational hypertension group,mild and severe preeclampsia group (0.82±0.27,0.92± 0.26 and 1.36±0.29,respectively) were significantly higher than that in the control group (0.45±0.18),with statistically significant differences (P＜0.05).The expression of placental HSP70 mRNA in severe preeclampsia group was significantly higher than that in gestational hypertension group and mild preeclampsia group(P＜0.05).Conclusion The expression of HSP70 increased significantly in maternal serum,umbilical cord blood and placenta of patients with HDCP,and it had positive correlation with the severity of the disease,indicating that HSP70 may play a role in the pathogenesis of HDCP.

Background and purpose:As a regulatory factor of cytoskeletal structure,a close correlation existed between Tiam 1(T lymphoma invasion and metastasis inducing factor 1) and the invasion and metastasis of gastroenteric tumor.We aimed to observe the effects of Tiam 1 antisense oligodeoxynucleotides(Tiam 1 ASODN) transfection on the invasive and migratory potentials of gastric cancer cells in vitro and vivo.Methods:The higher invasive and migratory subgroup(MH) were separated from human gastric cancer cell line MKN-45(M0) by laminin adhesion method in vitro.Tiam 1 ASODN was transfected into MH cells with liposome,and the expression of Tiam 1 mRNA and protein was determined by RT-PCR and ELISA separately.Changes in the invasive and migratory potentials of MH cells in vitro and vivo after transfected with ASODN were observed by Boyden chamber test and inoculation into nude mice respectively.Results:The expression of Tiam 1 mRNA and protein in MH cells after transfected with 0.43 ?mol/L ASODN(0.162?0.018,0.982?0.119) was significantly lower than that of either Liposome-transfected cells(0.789?0.054,1.237?0.108),sense oligodeoxynucleotides(SODN)-Liposome-transfected cells(0.754?0.039,1.234?0.103) or No-transfected cells(0.801?0.065,1.290?0.182)(P

Objective To investigate the relationship between the expression of T lymphoma invasion and (metastasis) inducing factor 1 (Tiam1), ras-related C3 botulinum toxin substrate 1 (Rac1) with the invasion and metastasis of gastric cancer. Methods The expressions of Tiam1 and Rac1 proteins in 60 cases of (gastric) cancer and paracarcioma gastric mucosa tissues were detected using Strept Avidin-Biotin Complex(SABC) immunohistochemical method,and the correlation between the expression of Tiam1, Rac1 and (clinicopathological) parameters of gastric cancer were analysed. Results (1)There was negative staining of Tiam1 protein in paracarcioma gastric mucosa tissues, while positive staining was detected in gastric cancer (tissues)(78.33%)(P 0.05). (4) The expression of Rac1 in patients with positive staining of Tiam1 was significantlyhigher than that in patients with negative staining(P

Response evaluation criteria in solid tumors is widely used for assessing tumor response to radiotherapy in clinical practice.However,it is clearly insufficient.Currently there is no ideal method for early evaluation of tumor radiation response.This paper reviews studies on early evaluation of radiotherapy efficacy by positron emission tomography-computed tomography (CT),magnetic resonance imaging,and CT.This paper also further explores the necessity and feasibility of CT values of megavoltage CT scans in early evaluation of the radiosensitivity of tumor during tomotherapy.

Objective To label human VEGF targeted bevacizumab (avastin) with 111In and to evaluate the application of 111 In?DTPA?avastin SPECT imaging for tumor diagnosis. Methods DTPA?avastin was prepared by coupling with a bifunctional chelating agent, and then labeled with 111 In to obtain 111 In?DTPA?avastin. The stability and molecular integrity of the labeled radiotracer were studied. Human hepatoma cell ( BEL7404) bearing nude mice tumor model was employed for tumor targeting evaluation. Gamma imaging was acquired after intravenous injection of 18.5 MBq probe. At the end of the observation, animals were sac?rificed for bio?distribution study. Results 111 In?DTPA?avastin tracer was synthesized and purified to a?chieve a radiochemical purity yield above 98% and specific activity up to 185 GBq/nmol. Its stability in 5%BSA was optimal, and the radiochemical purity after incubation for 96 h was over 90%. Gamma imaging re?sults showed that the tracer possessed definite tumor targeting property. Its biodistribution was consistent with that of normal in vivo antibody metabolism while possessing a good tumor?targeting property with a relatively high uptake of (3.8±0.8) %ID/g in tumor tissues 96 h after injection. Conclusions 111 In?DTPA?avastin tracer has good physicochemical properties, in vivo stability and good VEGF targeted binding. 111 In?DTPA?avastin has potential to be a new molecular probe for SPECT imaging.

Objective To investigate the anti-gastric cancer effects of dendritic cells tumor vaccine loaded with heat shock protein gp96 polypeptide complex. Methods Con A sepharose affinity chromatography and mono Q ion exchange chromatography were applied to purify gp96 polypeptide complex from gastric cancer tissues and DC was loaded with gp96 polypeptide complex to prepare tumor DC vaccine. Fluorescence-activated cell sorter (FACS) was used to detect surface molecules expression of dendritic cells. Enzyme-linked immunosorbent assay ( ELISA) was used to detect IL-10 and interferon-? (IFN-?) of the supernatant secreted by activated T lymphocytes and 51Cr release test was performed to evaluate the gp96 peptide specific CTL response. Results DCs derived from patient's peripheral blood mononuclear cells loaded with gp96 polypeptide complex had high express surface molecule of GD1? (79. 3 ?4. 1)% , CD80 (84. 3 ? 2. 4) % , CD83 (85. 7 ? 3. 2) % and HLA-DR( 83.4 ? 2. 9) %. The effector T lymphocytes induced by DCs vaccine loaded with gp96 polypeptide complex had rised killing effect to primary culture gastric tumourcells(58.47?10. 71)% when compared with SGC 7901 cells(23. 98 ?4. 16)%. The level of IFN-? (2875 ? 177.66 pg/ml) significantly increased and that of IL-10 (36 ? 6.72 pg/ml) decreased. Conclusion Autologous heat shock protein gp96 polypeptide complex/DC vaccine induces strong peptide complex specific CTL response with high specificity.

Objective To evaluate the impact of sepsis bundle treatment on morbidity of sepsis or septic shock in burned patients. Methods A retrospective clinical study was conducted on burned patients with severe sepsis or sepsis shock in burn intensive care unit. 95 patients were divided into control group(43 patients) and study group (52 patients)according to their treatment. The relationship between sepsis bundle index and sepsis, septic shock and 28-day mortality respectively were analyzed with logistic regression analysis. The compliance of sepsis bundles,and 28-day mortality were noted. Results The 28-day mortility rate,the mobidity of sepsis and septic shock in study group was lower than that of control group (P ＜ 0. 05). It could be found that 6-hour EGDT and 24-hour EGDT was the independent protective factor of sepsis and septic shock through logistic regression analysis ,and blood gas analysis, EGDT and vasoactive drug were correlated with 28-day mortality(P ＜0. 05). Compliance with sepsis bundles of 6-hour EGDT and 24-hour EGDT in study group was only 51.9%, and 63.2% respectively. Conclusion Sepsis bundle was able to improve survival rate in severe burn patients. Compliance with sepsis bundles in burn medical staff needed a buring improve.