Arthroscopy ; Humans ; Miners ; Oil and Gas Fields ; Osteoarthritis, Knee ; surgery

Objective To investigate the expression and clinical significance of matrix metalloproteinase 3 (MMP-3) and adiponectin in synovial fluids of knee osteoarthritis (OA) patients.Methods Ninety knee OA patients (OA group) were divided into Ⅰ-Ⅳ grade by Kellgren-Lawrenee (KL)standard,in which 21 patients of grade Ⅰ,27 patients of grade Ⅱ,23 patients of grade Ⅲ,19 patients of grade Ⅳ.The level of MMP-3 and adiponectin in synovial fluids of knee OA patients and 40 patients with knee meniscus injury (control group) were detected by ELISA method.Results The level of MMP-3 and adiponectin in OA group were (27.57 ± 16.53),(144.04 + 48.01) ng/L,significantly higher than those in control group [(11.27 + 3.76),(86.05 + 28.52) ng/L] (t =6.153,8.638,P ＜ 0.05).The level of MMP-3 and adiponectin in knee OA patients of Ⅰ-1Ⅳ grade were higher than those in control group,but the level of MMP-3 and adiponectin in knee OA patients of Ⅰ-Ⅱ grade had no significant difference compared with control group (P ＞ 0.05),the level of MMP-3 and adiponectin in knee OA patients of Ⅲ-Ⅳ grade had significant difference compared with control group (P ＜ 0.05).The level of MMP-3 was positively correlated with adiponectin (r =0.506,P ＜0.05).Conclusions The MMP-3 and adiponectin have abnormal expression in synovial fluids of knee OA patients.The abnormal expression is associated with occurrence and progression of knee OA.

Objective To investigate the clinical effects of arthroscopic repair and reconstruction of anterior cruciate ligament repair (ACL) combined meniscus injury. Methods 40 cases of patients with anterior cruciate ligament (ACL) combined meniscus injury were selected, they were divided into experimental group (also repair ACL and meniscus), control group (meniscus repair), the Lysholm scores before and after surgery of the two groups, as well as the postoperative Lysholm scores of the two groups were observed. Then compared the meniscal healing rate after surgery and meniscal reoperation rate after 2 years of the two groups. Results The postoperative Lysholm score after surgery was higher than before surgery, but the Lysholm score of the experimental group was higher than that in control group. The healing rate of the experimental group was higher than that in control group, the meniscus reoperation rate after 2 years was lower than that in control group. Conclusion Arthroscopic repair and reconstruction of anterior cruciate ligament (ACL) and meniscus injury can improve the cure rate, is conducive to postoperative rehabilitation, and the long-term effect is obvious, so is worthy of promoting.

Objective To investigate the effects of edaravone on the levels of high sensitivity C-reactive protein(hs-CRP) and D-dimer in patients with acute ischemic cerebral infarction. Methods A total of 86 patients with acute ischemic cerebral infarction were randomly divided and assigned into therapy group and control group. The control group was given a routine treatment of acute ischemic stroke. The therapy group was treated with edaravone in addition to the routine treatment. hs-CRP and D-dimer were measured before and after treatment. Barthel index(BI) and Chinese stroke Scale(CSS) of two groups were assessed before treatment and after treatment. Results The levels of hs-CRP and D-dimer were deceased after treatment. After the start of edaravone ,the levels of hs-CRP and D-dimer of therapy group were significantly lower than those of control group(P ＜0.05). The scores of CSS and BI of two groups improved after treatment,and scores of CSS and BI of the therapy group were better than those of the control group(P＜ 0.05). Conclusion Edaravone could decrease the levels of hs-CRP and D-dimer and is beneficial to improve neurofunctional recovery.

The efficacy of colorectal cancer treatment is not very satisfactory.Conventional morphologic imagings are limited in the diagnosis and in the applications of multimodality treatment for colorectal cancer.18 F-fluorodeoxyglucose PET-CT can play a pivotal role in detecting metabolism changes and in the localization of lesions concurrently.It is valuable in the assessment of responses to therapy,prediction of prognosis and selecting modality management and monitoring postoperative recurrence.The right-moment and proper application of PET-CT can increase the therapeutic efficacy of colorectal cancer.

Objective To evaluate the efficacy of three-dimensional conformal radiotherapy (3DCRT) combined with concurrent chemotherapy for loco-regionally recurrent or mastastatie rectal cancer. Methods Between June 2004 and January 2007,47 patients with loco-regionally recurrent or nmatastatic rectal cancer were treated by 3DCRT of 55-65 Gy in 1.8-2.0 Gy fractions. Chemotherapy was given concurrenfly using oxaliplatin(100 mg/m2 ,iv drop,d1 ) and capecitabine(1500 mg/m2,orally,dl-14,21 days per cycle). Results After the follow-up of 12-35 months, the total response rate, complete response rate and partial response rate were 79% (37/47) ,19% (9/47) and 60% (28/47) ,respectively. The pain-alleviation rate and the mean pain-alleviation time were 85% and 6 months. The 1- and 2-year survival rates were 83% and 51%. Quality of life was improved without any treatment related death. Conclusions 3DCRT combined with concurrent chemotherapy is effective and well-tolerated in patients with post-operatively locoregionally recurrent or mastastatic rectal cancer.

AIM: To investigate the apoptosis in primary gastric cancer cells induced by resveratrol, and the relation between this apoptosis and expression of bcl- 2 and bax. METHODS: In in vitro experiments, MTT assay was used to determine the cell gowth inhibitory rate. Transmission electron microscopy and TUNEL staining were used to quantitatively and qualitively detect the apoptosis of primary gastric cancer cells before and after the resveratrol treatment. Immunohistochemical staining and RT - PCR was used to detect the expression of apoptosis - regulated gene bcl - 2 and bax. RESULTS: Resveratrol inhibited the growth of primary gastric cancer cells in a dose - and time - dependent manner. Resveratrol induced primary gastric cancer cells to undergo apoptosis with typically apoptotic characteristics. TUNEL assay showed that after the treatment of primary gastric cancer cells with resveratrol for 24, 48, 72, 96 hours, the apoptotic indexs were 4.93% ± 0.19%, 16.74% ± 0.43%, 27.88% ±0.36%, 36.84% ± 1.07 % respectively. Immunohistochemical staining showed that after the treatment of primary gastric cancer cells with resveratrol for 24, 48, 72, 96 hours, the positive rates of Bcl - 2 proteins were 20.68% ± 0.49%, 10.84% ±0.33%, 6.80% ± 0.34%, 3.91% ± 0.15% and the positive rates of Bax proteins were 19.79% ± 0.98%, 30.74% ±0.85%, 40.14% ± 1.17%, 60.08% ± 1.64%. After exposed to resveratrol for 24 h, 48 h, 72 h and 96 h, the density of bcl- 2 mRNA decreased progressively with elongation of time and the density of bax mRNA increased progressively with elongation of time by RT- PCR. CONCLUSION: Resveratrol is able to induce the apoptosis in primary gastric cancer. This apoptosis may be mediated by down- regulation of Bcl- 2 and up- regulation of Bax.

Objective To identify and analyze the DNA methylation induced pathogenic genes in breast cancer.Methods Using genomic data provided by the international cancer genome,we carried out systematic a-nalysis of the abnormal gene expression in breast cancer and epigenetic regulation mechanism by bioinformatics methods.Results We identified 428 genes with abnormal expressions in breast cancer by t test.Functional enrich-ment analysis revealed genes up-regulation in breast cancer were closely related to the cell cycle,and those down-regulated were significantly enriched in the hormone response function.Study on DNA methylation revealed that breast cancer showed an unique DNA methylation pattern.Further analysis reveals 23 breast cancer genes induced by abnormal DNA methylation.Conclusion DNA methylation can mediate the abnormal expression of breast canc-er genes,and is an important biological marker for early diagnosis and prognosis of breast cancer.

OBJECTIVE:To explore the spasmolytic effect of baked Radix Vladimiriae on in vitro intestinal muscle in rabbits. METHODS:Magnus in vitro intestinal muscle accumulative dose method was adopted to observe the dose-effect relationship of petroleum ether parts of baked Radix Vladimiriae with in vitro intestinal muscle of rabbits and its impact on the spasm of rabbit intestinal muscle induced by acetylcholine, histamine phosphate and Bacl2. RESULTS:There was a certain dose-effect relationship between petroleum ether parts of baked Radix Vladimiriae and spasmolytic mechanism of rabbit in vitro intestine. It showed the effect of acetylcholine, histamine phosphate and Bacl2. CONCLUSION:The inhibition effect of petroleum ether parts of baked Radix Vladimiriae on normal in vitro intestinal muscle increase as long as dose increase. The inhibition mechanism may be associated with acetylcholine competing with histamine to block M receptor and H1 receptor as well as to inhibit excitability of intestinal muscle.