OBJECTIVETo optimize the combination modes of medical materials in extract of Huanglian Jiedu decoction.

METHODDivided the medical materials of Huanglian Jiedu decotion into 15 groups and extracted by semi-bionic estraction (SBE), the contents of berberine, baicalin, geniposide, total alkaloid, total flavonoid, total integral calculus area of HPLC and extract of 1 000 were determined, standardized, weighted and evaluated, respectively.

RESULTThe maximum evaluated value Y for the extracting technique was AD + BC.

CONCLUSIONThe optimal combination mode of the perscription is that Rhizoma Coptidis and Fructus Gardeniae, Radix Scutellariae and Cortex Phellodendri Amurensis are decocted together respectively then the two decoctions are merged.

Coptis ; chemistry ; Dosage Forms ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Gardenia ; chemistry ; Phellodendron ; chemistry ; Scutellaria baicalensis ; chemistry