Patients found two years left of the new biology, surface erosion,exudates long. Postoperative pathology examination: inflammatory fibrous background visible uniform small cells arranged in cords, pseudorosette formation region is considered not except basal cell carcinoma , neuroendodrne carcinoma. Diagnosis of immunohistochemical support of basal cell carcinonma.
Carcinoma, Basal Cell ; diagnosis ; pathology ; Humans ; Immunohistochemistry ; Skin Neoplasms ; diagnosis ; pathology

The oral administration of bioactive macromolecular drugs such as proteins, peptides and nucleic acids represents unprecedented challenges from the drug delivery point of view. One key consideration is how to overcome the gastrointestinal tract absorption barrier. Recent studies suggest that microfold cell (M cell), a kind of specialized antigen-sampling epithelial cell which is characterized by a high endocytic rate and low degradation ability, may play an important role in macromolecule oral absorption. The development of an in vitro M cell coculture system and its modified models greatly advanced the study of M cells and the development of oral delivery system for macromolecular drugs. The special structure, function and formation characteristics, and biomarkers of M cell are summarized in this review. The applications of in vitro M cell models in developing oral delivery system ofbioactive macromolecular drugs are discussed.

Objective To clone ,construction ,express and purify Tp47 of Treponema pallidum (Tp) ,and assess the immunoreac‐tivity by Western‐Blot .Methods Tp47 was amplified by polymerase chain reaction ,and then cloned to the vector pGEX‐6P‐1 .The correct sequence of the recombinant plasmids pGEX‐6P1‐Tp47 was transformed into Escherichia coli BL21 (DE3) and induced .The expression product was analyzed by sodium dodecyl sulfate polyacrylamide gel electropheresis and Western‐Blot .The expression protein was purified .Serum of different clinical stages of syphilis was used as the antibody to detect the immunoreactivity of the protein by Western‐Blot .Results A fusion protein with molecular weight about 71 × 103 was attained .Western‐Blot proved that the recombinant protein can react with Tp IgG positive sera .And the specificities and sensitivities of the diagnostic reagent detected by sera were 100% .Conclusion The recombinant protein Tp47 was expressed and purified with good antigen activity ,which could provide the basis of theory and practice for the development of early diagnostic kit applying to detect Tp infection .

Objective To explore the effect of traction fixation method of pubic balloon ureter on postoperative complication of transurethral resection of the prostate .Methods Selected 367 patients with traction fixation method of pubic balloon ureter from January 2012 to June 2013 as observation group , and 332 patients from July 2010 to December 2011 as control group with thighs fixed method of balloon catheter .Then, the complication of postoperative complication and patients comfort level were observed and compared between two groups . Results The red blood cells in the urine per high power field in observation group was (45.6 ±10.4), and that in control group was (52.0 ±13.1), and the difference was statistically significant ( t =11.104,P <0.05).The incidence of cystospasm , ureter blocking, thrombus of lower extremity veins in observation group (9.8%,6.8%,0.0%) was lower than the control group (28%,10.8%,1.2%), the differences were statistically significant(χ2 =27.041,29.234,4.457, repectively;P<0.05).Conclusions Application of traction fixation method of pubic balloon ureter in transurethral resection of the prostate can reduce the postoperative complications such as blooding , cystospasm, ureter blocking and so on, which also can improve the comfort level of patients.

ObjectiveTo analyze the research status and trends in low-level occupational benzene exposure. Methods Articles on low-level occupational benzene exposure from Chinese and English journals from January 1st, 2000, to December 31th, 2022 were retrieved using the Web of Science and the China National Knowledge Infrastructure, and a bibliometric analysis was conducted. Results A total of 327 articles were included in the analysis, comprising 216 English articles and 111 Chinese articles. i) The number of articles published in English fluctuates greatly over the years, without a trend of continuous growth or decline. Authors from 359 research institutions in 45 countries and regions have published relevant English articles in 97 kinds of journals, involving 281 grants from 226 foundations. The top three countries in terms of articles amount were the United States, Italy, and China, with 81, 46, and 43 papers, respectively. The English articles mainly focused on mechanistic research at the genetic level, such as hematotoxicity, oxidative stress, and DNA damage. ii) The number of Chinese articles increased gradually after 2012, with the growth peak in 2017. Authors from 127 research institutions in 26 provinces, autonomous regions, and municipalities published Chinese articles in 51 kinds of journals, involving 154 grants from 78 foundations. Chinese articles tended to focus on benzene-induced hematotoxicity and occupational health damage. Conclusion Most studies on low-level occupational benzene exposure were conducted in China, the United States and Italy, focused on hematotoxicity. Monitoring international research topics and hotspots of the field has certain reference value for related research in China.