Recent in vitro and in vivo experiments have revealed that ellagic acid and its metabolites can inhibit the growth of digestive system malignant tumor cells by inhibiting tumor cell proliferation,inducing apoptosis,breaking DNA binding to carcinogens,blocking virus infection,disturbing inflammation,angiogenesis and drug-resistance processes required for tumor metastasis.Ellagic acid and its metabolites are potential chemoprevention and therapeutic drugs against human cancers.

Well-designed operation and management system of research laboratories is essential for scientific innovations and talent training.The reform of the traditional laboratory management to encourage science innovation and development is key to promoting the laboratories to develop in scale,level and efficiency.On the basis of profiling traditional lab management and its setbacks,the paper described the management mode and operation mechanism of Capital Medical University.Such mode and mechanism feature lab project Principal Investigation responsible system,and shared platform for large equipments and services.The paper also discussed lab operation and management system for universities at large.

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Following injection of HRP into the cerebellum of the rat, the retrogradely labeled′spinal border cells′ in L_1-L_3 of the spinal cord were examined electron microscopically for understanding the synaptology. Six types of terminal boutons (S, F, Cf, T, G and P types) which terminated on the surface of cell bodies or dendrites of the spinal border cells were recognized. The S- and F-boutons contain spherical and elliptical vesicles respectively. These two types of boutons had relatively broad area contacting with the surface of either cell bodies or dendrites. They could be derided into two sub-types respectively. One was the elonagted giant bouton and the other was the round. Cf-type contain flattened vesicles and it make membraneous′contact′ lacking in specialized pre-and postsynaptic membrane thickenings. T-type contain spherical and large granular vesicles, and dense body was seen beneath the post synaptic membrane. G-type boutons contain large granular vesicles in area of presynaptic. P-type boutons form synapses upon the large S-type boutons and contain pleomorphic vesicles. The postsynaptic membrane of S-, T- and G-types is apparently thicker than the presynaptic membrane and showed to be asymmetrical. Further study is necessary with regard to the sources of different terminal boutons contacting with different portions of the spinal border cells.

Objective To explore the mechanisms of trafficking and signaling of serotonin 1A receptor(5-HT_(1A))and its spatiotemporal distribution in living cells.Methods The mouse 5-HT_(1A) gene amplified by RT-PCR was recombined into pEGFP-N1 vector and the EGFP coding sequence was located in-frame at the C-terminal end of the 5-HT_(1A) receptor.The 5-HT_(1A)-EGFP was transfected into neuron-like PC12 cells as well as HEK293.The transfected cells were visualized using confocal microscopy,the mobility of 5-HT_(1A)-EGFP was monitored by live measurements and fluorescence recovery after photobleaching.Results The 5-HT_(1A) gene was identitical with the published gene sequence NM_008308.4 and a 5-HT_(1A)-EGFP fusion construct was created.After stable transfection of the plasimd into a PC12 cell line and analysis with a confocal laser scanning microscopy,the EGFP-tagged 5-HT_(1A) was predominantly associated with the plasma membrane,but some intracellular vesicles in the perinuclear region also contained the fusion protein.The predominant localization of 5-HT_(1A)-EGFP at the plasma membrane was confirmed in transiently transfected HEK293 cells.Bleached fluorescence was partialy recovered in 100 seconds,indicating that the 5-HT_(1A)-EGFP was mobiled on the membrane.Conclusion Spatiotemporal distribution and mobility of 5-HT_(1A) tagged with EGFP can be monitored in the 5-HT_(1A)-EGFP stable PC12 cell line,which could be an excellent neuron-like experimental cell model for research of 5-HT_(1A) trafficking and signaling.

Long non-coding RNAs(lncRNAs)is a functional RNA molecule with a sequence length greater than 200 nucleo-tides that is not translated into a protein. LncRNA is of great value in the clinical application of malignant tumors and is closely related to the diagnosis,prevention,treatment and prognosis of tumors. CCAT1(human colon cancer associated transcript-1)can play a carci-nogenic role by promoting cell proliferation,invasion and migration. CCAT1 can be used as a biomarker for tumor prognosis. In this re-view,the research progress on the relationship between CCAT1 and digestive system malignant tumors is reviewed in recent years,the current status and prospects of malignant tumor treatment by CCAT1 are discussed.

Objective To investigate antimicrobial resistance and pathogen in hebei antibacterial resistance investigation net in 2012.Methods Antimicrobial susceptibility test was detected by Kirby-Bauer method or broth dilution test.Results were analyzed according to CLSI 2010 breakpoints.WHONET 5.5 software was used to analyze the data.Results A total of 10 504 clinical isolates were collected in 2012,of which gram negative bacilli and gram positive cocci accounted for 76.2%, 23.8%,respectively.The most common pathogen in gram-negative rod was E.coli,K.pneumoniae,P.aeruginosa, A.baumanii and E.cloacae respectively.The most common pathogen in gram-positive cocci was S.aureus,E.facium,E-.faecalis,S.pneumoniae and S.epidermidis.ESBL rate of E.coli and K.pneumoniae was 66.5 and 46.7%.The resistant rate of E.coli,K.pneumoniae,E.cloacae to imipenem was 0.1%,0.5%,8.9% and to meropenem was 0.1%,0.6%,4.2%, respectively.P.aeruginosa was resistant to imipenem and meropenem were 38.9% and 32.3%.A.baumanii was resistant to imipenem and meropenem were 5 6.5% and 5 9.7%.Methicillin-resistant strains accounted for an average of 5 7.5% in S.aureus and 87.3% in coagulase negative staphylococcus.Staphylococcus was still susceptible to minocycline and chloram-phenicol.No staphylococcal strains were found resistant to vancomycin,linezolid.But a few coagulase negative staphylococcal strains were resistant to teicoplanin.Conclusion Surveillance of antimicrobial agents played an important role in controlling hospital infection.

Intensive cancer treatment with drug combination is widely exploited in the clinic but suffers from inconsistent pharmacokinetics among different therapeutic agents.To overcome it,the emerging nanomedicine offers an unparalleled opportunity for encapsulating multiple drugs in a nano-carrier.Herein,a two-step super-assembled strategy was performed to unify the pharmacokinetics of a pep-tide and a small molecular compound.In this proof-of-concept study,the bioinformatics analysis firstly revealed the potential synergies towards hepatoma therapy for the associative inhibition of exportin 1(XPO1)and ataxia telangiectasia mutated-Rad3-related(ATR),and then a super-assembled nano-pill(gold nano drug carrier loaded AZD6738 and 97-110 amino acids of apoptin(AP)(AA@G))was con-structed through camouflaging AZD6738(ATR small-molecule inhibitor)-binding human serum albumin onto the AP-Au supramolecular nanoparticle.As expected,both in vitro and in vivo experiment results verified that the AA@G possessed extraordinary biocompatibility and enhanced therapeutic effect through inducing cell cycle arrest,promoting DNA damage and inhibiting DNA repair of hepatoma cell.This work not only provides a co-delivery strategy for intensive liver cancer treatment with the clinical translational potential,but develops a common approach to unify the pharmacokinetics of peptide and small-molecular compounds,thereby extending the scope of drugs for developing the advanced com-bination therapy.