Objective:To explore diagnostic value of 6min walking test (6MWT) for coronary heart disease (CHD) . Methods :A total of 192 patients suspected of CHD ,including 123 men and 69 women ,who were treated in our hos‐pital ,were selected .After hospitalization ,all patients received 6MWT and coronary angiography (CAG) examina‐tion .With CAG results as the gold standard for diagnosing CHD ,sensitivity ,specificity ,accuracy ,positive predic‐tive value and negative predictive value of 6MWT diagnosing CHD were calculated ,and they were compared be‐tween men and women .Detections of 6WMT among groups of different diseased number of vessels were compared . Results :With CAG results as the standard ,accuracy ,specificity ,sensitivity ,positive predictive value and negative predictive value of 6MWT diagnosing CHD were 71.35% ,80.58% ,60.67% ,72.97% and 70.34% respectively . Compared with female patients ,there were significant rise in accuracy (57.97% vs .77.24% ) ,sensitivity (39.29%vs .70.49% ) ,specificity (70.73% vs .83.87% ) and positive predictive value (47.83% vs .81.13% ) , P<0.05 or<0.01 ,and significant reductions in false positive rate (29.27% vs .16.13% ) and false negative rate (60.71% vs . 29.51% ) in male patients (P<0.05 or <0.01) ,there was no significant difference in negative predictive value be‐tween two groups (P>0.05) .Compared with single - and double -vessel coronary disease ,there was significant rise in true positive rate (43.75% ,54.05% vs .100% ) of triple - or multi -vessel coronary disease diagnosed by 6MWT , P< 0.01 both . Conclusion:The 6min walking test possesses high specificity and sensitivity diagnosing CHD ,and its diagnostic value is higher for male patients and patients with serious condition .Its procedures are sim‐ple with low cost and no damage to patients ,which can be used as adjunctive examination for CHD diagnosis and ex‐tended in clinic .

OBJECTIVE To analyze the drug-resistance of Escherichia coli isolated from clinic in the past two years. METHODS The drug-resistance to 19 kinds of antimicrobial agents of 435 E. coli strains was detected by K-B method. 3-D test was performed to detect AmpC ?-lactamase and Extended-spectrum ?-lactamases(ESBLs). RESULTS It showed that the strains from cervical secretions, urine, throat swab and lochia specimens had the highest positive rate. Sensitivity test results showed that the resistance rate to ampicillin, aztreonam, sulfamethoxazole/trimethoprims and quinolones was high (49.23-84.10%), and resistance rate to the third, and fourth-generation cephalosporins was high too(31.35-46.80%). Strains that resistant to imipenem and meropenem were not detected out and they had a high sensitivity rate (93.38%), to cefoperazone/sulbactam. Detection rate of ESBLs and AmpC was 32.67% and 5.52%, respectively. Nineteen srtains produced both of them and 6 srtains produced AmpC only. CONCLUSIONS The detection rate and drug resistance are increasingly severe. Clinic and laboratory should make efforts to reduce hospital infection and dissemination of drug-resistant strains.

Camptothecin/*pharmacology ; Liver Neoplasms, Experimental/*immunology ; Neoplasm Transplantation ; Sarcoma, Experimental/*immunology ; Transplantation, Isogeneic

Objective To evaluate the feasibility and the safety of thulium laser vaporization enucleation of prostate(TVEP) combined with traditional transurethral resection of prostate (TURP) for therapy benign prostate hyperplasia (BPH) with its volume larger than 80 millilitres.Methods Twenty-five BPH patients (volume larger than 80 millilitres) underwent TVEP combined with TURP.The operation time,intraoperative blood loss,enucleation time,cutting time,bladder irrigating time,catheterization time,perioperative and 6 months' follow-up data such as the international prostate symptom score,quality of life score,the maximum urinary flow rate,the residual urine volume and so on were observed.Results The operation time was (66 ± 26) min.The enucleation time was (25 ± 9) min.The cutting time was (32 ± 8) min.The intraoperative blood loss was (140 ± 25) ml.The bladder irrigation time was (3.0 ± 1.0) d.The catheterization time was (5.7 ± 1.0) d.After 6 months,the maximum urinary flow rate,residual urine volume,international prostate symptom score and quality of life score were improved:(18.7 ± 1.7) ml/s vs.(6.8 ± 1.7) ml/s,(18.9 ± 1.8) ml vs.(65.7 ±8.1) m1,(8.7 ± 1.6) scores vs.(25.7 ±4.3) scores,(1.7 ± 1.2) scores vs.(4.7 ± 1.1) scores,and there were significant differences (P ＜ 0.05).Urethral stricture developed in 2 patients and epididymitis happened in 3 patients.No blood transfusion events and transurethral electric cutting syndrome occurred.Conclusion TVEP combined with TURP for therapy BPH larger than 80 millilitres is safe,and the incidence of complications is low.

OBJECTIVE To understand the clinical distribution and drug resistance of Enterococcus to provide evidence for the rational use of antibiotics. METHODS The Enterococcus isolated form clinic from Jan 2006 to Dec 2007 and the sensitivity to 13 kinds of antibiotics were tested by K-B method. RESULTS All of 122 strains of Enterococcus were isolated which included 76 strains of E.faecalis(62.3%),29 strains of E.faecium(23.8%) and 17 strains of other enterococcus(13.9%).Most enterococcus were detected from vaginal secretion,urine,drainage fluid,cervical secretion and throat swab.Drug sensitivity test showed that the sensitivity to vancomycin and teicoplanin was high while the resistance to rifampicin,erythromycin,tetracycline and ciprofloxacin was the highest,and the total resistance rate of E.faecium was higher than E.faecalis. CONCLUSIONS The detection rate and resistance rate of Enterococcus present a rising trend,which should be paid for more attention by clinic.Bateria cultivation is necessary for suspected enterococcus infection and the drugs selected according to sensitivity result to improve the curative effect.

OBJECTIVE To understand the pathogen distribution of infant infectious bacterial conjunctivitis and keratitis,and drug resistance to provide guidence on clinical treatment.METHODS The routine bacterial culture of eye secretions in the 331 cases was identified with K-B to determine the resistance of pathogenic bacteria.RESULTS A total of 124 strains were isolated with positive rate of 37.46%,there were Haemophilus parainfluenzae,Staphylococcus aureus,H.influenzae,S.epidermidis,fungi,etc.The resistance of S.aureus,S.epidermidis and S.pneumoniae to penicillin were 86.36%,88.89% and 0,respectively.The resistance rate of H.parainfluenzae,H.influenzae,Escherichia coli,Klebsiella pneumoniae and Pseudomonas aeruginosa to ampicillin were 12.50%,27.78%,87.50%,100.00% and 100.00%;that to cefazolin were 16.67%,16.67%,62.50%,14.29% and 100.00%;and to cotrimoxazole were 50.00%,27.78%,12.50%,14.29% and 100.00%.CONCLUSIONS The H.parainfluenzae,S.aureus,H.influenzaeand S.epidermidis are isolated from the newborn eye secretions;they were lower resistant to ofloxacin and ciprofloxacin.

OBJECTIVE To investigate the distribution and drug resistance of pathogenic bacteria which caused respiratory infection among suscepted patients and offer scientific basis for reasonable usage of antibiotics.METHODS Oropharyngeal swabs among 709 cases of respiratory infection neonates were investigated by the routine methods and drug resistance was analyzed by K-B method.RESULTS Totally 438 bacterial strains were isolated from 709 neonates.most of these bacteria were Gram-negative bacilli(70.3%),among which Haemophilus influenzae and Klebsiella pneumoniae were accounted for 39.8% and 10.3%,respectively;fungi and Gram-positive cocci were accounted for 23.5% and 6.2%.CONCLUSIONS Most strains present higher resistance rates to penicillin and ampicillin;but cefoxitin,amikacin,vancomycin,imipenem and the third generation cephalosporins are revealed with higher sensitivity rates for pathogenic bacteria in newborns.

Objective To express and purify EGF-TCS fusion protein and observe the targeted and selective killing effect on cancer cells of the fusion protein.Methods The recombinant expression plasmid PQE30/EGF-TCS was transformed to E.coli.M15 and the fusion protein(EGF-TCS) was expressed.Ni-NTA Agrose affinity chromatography was used to purify the protein,flow cytometry to detect EGFR expression rate in cancer cells(BEL-7402,MCF-7,BGC-823) and normal liver LO2 cells,and the killing test to verify selective killing ability of EGF-TCS;The cell apoptosis detection by flow cytometry and microscopic observation were used to confirm the selective killing ability of EGF-TCS.Results Recombinant expression plasmid PQE30/EGF-TCS was expressed in E.coli.M15 stably and effectively.The purity of EGF-TCS was over 95% by chromatography.EGFR expression rate was highest in hepatoma cells BEL-7402(72.33%) and lowest in normal liver LO2 cells(5.51%).The killing ability of recombinant protein was more effective to cancer cells(IC50 of BEL-7402,MCF-7 and BGC-823 was 11.4,22.47 and 12.53 ?g/ml respectively) and was weak to normal cells(IC50 53.19 ?g/ml).Conclusion The recombinant protein EGF-TCS that induces apoptosis of cancer cells was successfully constructed by gene engineering technology.

OBJECTIVE To understand the urinary tract infections after cesarean section,the distribution of pathogenic strains,and drug resistance analysis in order to provide clinical treatment. METHODS The routine urine cultures of the 2652 case were identified and drug resistance was analyzed by K-B method. RESULTS From 458 isolated bacteria,Gram-negative bacilli were main,pathogen accounting for 80.13%,Escherichia coli and Klebsiella,accounted for 71.62% and 4.8% respectively;Gram-positive bacteria accounted for 14.41%,and fungi accounted for 3.71%. CONCLUSIONS G-bacteria in the urinary tract infection are in the absolute superiority;rational use of antibiotics to effectively control the urinary tract infections and avoid drug-resistant strains produceing is especially critical.

Objective To observe the clinical efficacy of Tiaoxinyin treatment for diabetic cardiac autonomic neuropathy (DCAN);To evaluate its efficacy and safety.Methods One hundred patients of DCAN were randomly divided into experimental group (51 cases) and control group (49 cases). Both groups were given basic treatment of diet control and Metformin hydrochloride tablets orally taking. Patients in the experimental group were given Tiaoxinyin, while patients in the control group were treated with Mecobalamin tablets for 8 weeks. Changes and untoward effects in blood glucose, blood fat and heart rate variability were observed before and after treatment so as to compare the efficacy of two groups.Results The total effective rate of the experimental group was 82.35% (42/51) and the control group was 63.27% (31/49), with statistical significance between the two groups (P<0.01). The TCM scores of the two groups decreased after treatment, with statistical significance compared with those before treatment (P<0.01). The difference in TCM scores between the two groups was significance (P<0.05). The level of FBG and 2 h PG in the two groups declined (P<0.05). Heart rate variability had statistical significance compared with that before treatment (P<0.05), with statistical significance between the two groups (P<0.05). No obvious adverse reaction was found.Conclusion Tiaoxinyin is effective and safe in treating DCAN and can improve the heart rate variability indicators in patients.