Objective To explore the clinical significance of interleukin (IL)- 17 in nasopharyngeal secretions (NPS) and serum of wheezing children under 5 years old. Methods Fifty-three children with recurrent wheezing under 5 years old were divided into wheezing group Ⅰ (with atopic high risks,27 cases)and wheezing group Ⅱ (without atopic high risks, 26 cases) ;20 children without infectious diseases such as hernia and renal calculus were enrolled as control group. After collecting and dealing their NPS and serum,the levels of IL-17 were measured by double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Results The levels of IL-17 in serum and NPS in wheezing group Ⅰ [(1469.32 ± 978.30),(1473.70 ±974.35) ng/L],and wheezing group Ⅱ [(263.34 ± 131.80), (788.28±132.40) ng/L] were significantly higher than those in control group [(36.48 ± 2.00), (36.45 ± 5.00) ng/L] (P ＜ 0.01 ), and the levels of IL- 17 in wheezing group Ⅰ were higher than those in wheezing group Ⅱ (P ＜ 0.05 ); the expression of IL- 17 in NPS and serum had a positive correlation (r = 0.313,P ＜ 0.05). Conclusion The detections of IL-17 in NPS can be used as laboratory index to distinguish wheezing children who have a tendency to persistent wheezing, and early intervention and treatment should be given.

BACKGROUND: In recent years, al kinds of absorbable internal fixation materials have been used in the clinical treatment of patel ar transverse fractures. OBJECTIVE: To investigate the repairing effect of absorbable internal fixation materials on patel ar transverse fractures. METHODS: Total y 102 cases of fresh patel ar transverse fractures were included 62 males and 40 females cases, with a mean age of (41.85±11.15) years, and according to treatment methods, they were divided into two groups: observation group (n=52) using absorbable screws for reconstruction and control group (n=50) using Kirschner wire repair. At 12 months after repair, Lysholm knee function score were measured and evaluated, and the occurrence of adverse events was recorded. RESULTS AND CONCLUSION: The knee function score and excel ent rate of the observation group were significantly higher than those of the control group (both P < 0.05). In the observation group, there were four cases of fracture displacement, but no skin irritation and other adverse events occurred; in the control group, there were two cases of nonunion and five cases of skin irritation or internal fixation loosening and slippage. Therefore, there was a higher incidence of adverse events in the control group compared with the observation group (P < 0.05). These experimental results show that the use of absorbable screws in the repair of patel ar transverse fracture can promote the functional recovery of the knee joint, with good biocompatibility.

Objective　To explore the effects of treatment and mechanism of sodium salicylate on blast deafness. Methods　Guinea pigs were divided into blast group, blast plus sodium Salicylate group and sodium solicylate group. The animals in the blast group were exposed to blast average peak pressure 176.7 dB(SPL); sodium salicylate was injected IP 400 mg/kg,1/d. Monitoring ABR threshold, the value of MDA of and counting the number of hair cells on the cochlea preparation, SEM for hair cells was observed. Results　The ABR threshold shift in the blast group was 46.75±13.56 dB，38.0±12.4 dB,35.5±10.67 dB，41.5±12.57 dB,33.75±8.42 dB, post-blast 1 d，3 d，1 w，2 w,4 w respectively. And that of the blast plus sodium salicylate was 46.65±11.7 dB，38.25±9.52 dB,30.5±9.17 dB,30.0±9.17 dB,23.0±6.57 dB. The two groups differed significantly at 1 w，2 w，4 w(P＜0.05). The value of MDA differed significantly at 2 w,4 w(P＜0.05). And the hair cells lost also differed significantly. It was observed that sodium salicylated can raise the ABR thresbold temporarily 24 hrs during simple sodium salicylate adminastration. However it recovered 14 hrs after the injection.Conclusion　Sodium salicylate can partially attenuate the hearing loss caused by blast. It is probable that sodium salicylate can clear peroxicative produced during blast and therefore protect hair cells and make hearing loss partially recover.

Objective To investigate the relationship between [Ca2 +]i and gentamicin ototoxicity. Methods Isolated outer hair cells were loaded with [Ca2 +]i - sensitive fluorescence dye Fluo- 3, and confocal laser microscopy was used to determine [Ca2 +]i when K +- Hanks、gentamicin or its metabolite were added. Results Gentamicin decreased [Ca2 +]i in short time, and inhibited inward calcium current induced by K + . On the contrary, metabolized gentamicin increased [Ca2 + ] i for a long period. It did not effect calcium current induced by K + . Conclusion Metabolized gentamicin increased [Ca2 + ]i, which may result in its ototoxicity.

OBJECTIVE:To discuss the dispensing of prescriptions of traditional Chinese medicine.METHODS:According to the dispensing procedure of Chinese traditional medicine and the principle of drug use,the problems occurred during Chinese traditional medicine dispensing were pointed out and analyzed.RESULTS:The problems occurred in the dispensing of prescriptions of traditional Chinese medicine were mainly resulted from doctors,pharmacists and nurses' lack of basic rationale or they had no intimate knowledge of operation regulations.These problems can be avoided if with efforts.CONCLUSION:Only through strictly following the prescription of Chinese traditional medicine can the quality and efficacy of Chinese drugs dispensing be guaranteed.

Objective To develop autosomal SNP fluorescent-multiplex PCR system,and investigate the allele frequencies of these 13 SNP loci in Southern Liaoning Han population.Methods After selected 13 autosomal SNP loci,SNP fluorescent-multiplex system was developed based on the principle of fragment length discrepant allele specific PCR,and the allele frequencies of these SNP loci in Southern Liaoning Han population were investigated using the established system.Results For the same SNP locus,the homozygote showed a single product peak,and the heterozygote showed two product peaks with different length.For the different SNP loci,the lengths of their PCR products were different.Therefore,we could genotype the 13 SNP loci simultaneously according to the length of products and the amount of product peaks,and the results were in accordance with that of direct sequencing.In the meanwhile,the allelic frequencies of these 13 SNP loci in Southern Liaoning Han population were obtained.Conclusion The SNP fluorescent-multiplex system based on the fragment length discrepant allele specific PCR strategy is simple and economical,and is of a high application value in forensic medicine.

Objective To examine the effect of family environment and family function on suicide behavior in schizophrenic patients.Methods 53 schizophrenic patients with suicidal behavior in the last month were selected for the schizophrenic suicide group.53 schizophrenic patients and 53 normal controls matched with the schizophrenic suicide group were selected for the schizophrenic no suicide group and normal control group.All of them completed family environment scale and family assessment device.Results The cohesion factor,the expressiveness factor,the active-recreational orientation factor and the control factor of family environment scale in the schizophrenic suicide group were significant lower than those in the schizophrenic without suicide group(separately 5.70 ± 1.29 vs 6.62± 1.23,4.77 ± 1.49 vs 5.96 ± 1.11,3.94 ± 1.63 vs 5.68 ± 1.98,2.68 ± 1.68 vs 4.40 ± 1.81,P＜ 0.05),but the conflict factor in the schizophrenic suicide group was higher than in the schizophrenic without suicide group(3.55 ± 1.60 vs 2.81 ± 1.52,P ＜ 0.05).The affective responsiveness factor,the affective involvement factor and the behavior control factor of family assessment device in the schizophrenic suicide group were significant lower than those in the schizophrenic without suicide group(separately 16.05 ± 2.57 vs 14.53 ± 3.38,19.13 ± 4.09 vs 16.58 ± 2.82,23.35 ± 3.52 vs 21.60 ± 3.25,P ＜ 0.05).Multiple liner regression analyses showed that the expressiveness factor,the active-recreational orientation factor,the control factor,the affective involvement factor and the behavior control factor were important factors for schizophrenic suicide(F=21.583,P=0.000,adjusted R 2 =0.519).Conclusion Attempted suicide patients with schizophrenia have some deficiencies at family function and family environment.

As the new force among university faculties,the young faculties are the backbone of teaching and research activities in universities and the main information users concerned for information access.To understand the actual condition of information literacy of young faculties in Peking University Health Science Center and grasp their difficulties in getting access to information,this study analyses the information literacy of young faculties through investigating the status quo of their information capabilities,gathering their suggestions and opinions to enhance information capabilities and retrieval tests conducted among small-scale young faculties.The survey results show that 72％ of young faculties don't know or know little about the four biomedical literature retrieval databases and 47％ of them knowing nothing about CALIS.This suggests we should take measures to promote the information literacy of young faculties.

Objective To explore the relationship of autoantibodies against G protein coupled angiotensin Ⅱ-1 receptor (AT1 R),α1-adrenergic and β1-adrenergic receptors (α1 R and β1 R) with thyrotoxicosis heart disease (THD).Methods The epitopes of the second extracellular loop ofAT1 R (165-191),α1 R (192-218),and β1 R(197-222) were synthesized for screening autoantibodies from 277 participants by ELISA.237 patients with thyrotoxicosis were subdivided into thyrotoxicosis treatment group (n =148) and thyrotoxicosis recovery group (n =89),or into THD group (n =46) and simple hyperthyroidism group (n =191).40 healthy subjects were served as control group.Results (1) The positive rates of autoantibodies against AT1 R,α1 R and β1 R in thyrotoxicosis patients were higher than those in the control subjects (31.6％ vs 12.5％,27.8％ vs 10.0％,and 23.6％ vs 7.5％,all P＜0.05).The positive rates of the three autoantibodies in the patients with Graves' disease were higher compared with thyrotoxicosis caused by other reasons (36.3％ vs 19.7％,32.2％ vs 16.7％,and 28.1％ vs 12.1％,all P＜0.05).(2) In thyrotoxicosis treatment group,the positive rates of autoantibodies against AT1 R and α1 R were higher than those in the hyperthyroidism recovery group (40.5％ vs 16.9％ and 33.1％ vs 19.1％,both P＜0.05).(3) The incidence of autoantibodies against AT1 R and α1 R in THD were significantly higher compared with simple hyperthyroidism (52.2％ vs 26.7％ and 43.5％ vs 24.1％,both P＜0.05).Conclusions Autoantibodies against AT1 R,α1 R,and β1 R may play important roles in the pathogenesis of hyperthyroidism,which may be involved in the progression of THD.

ObjectiveIn order to investigate the effects of shock wave on atrial natriuretic peptides immunoreactivity in the stria vascularis and hair cells of cochlear and correlation of auditory threshold shift,to study the mechanism of deafness caused by blast trauma. MethodsAfter shock wave, different groups of guinea pigs were studied by ABC immunohistochemistry, image analysis and auditory brainstem response. ResultsANP - IR optic density in experimental group was higher than that of control in the stria vascularis and inner hair cells. The change of ANP- IR optic density was directy correlated with ABR threshold shift (r2 =0.9185. P2＜0.05). There was no change of ABR and ANP - IR optic density on outer hair cells. ConclusionThe change of ANP in the stria vascularis may be a compensatory mechanism to heating lose caused by shock wave; ANP in inner cells may be a pathological manifestation of shock wave. The changes of ANP - IR in outer cells may be a false appearance, it was the shock wave which lead to outer cells ANP running off.