Objective To investigate the effects of peroxisome proliferator activated receptor γ(PPARγ) agonists pioglitazone combined with radiotherapy on colon carcinoma xenograft in mice.Methods The colon cancer cells CT26 of mouse were inoculated in the right side of the rib loins of BALB/c mouse.Established BALB/c mice tumor models.The mice were randomly divided into six groups:untreated group,tumor contral group,DMSO group,pioglitazone treated group,radiotherapy group,radiotherapy combined with pioglitazone treated group.To observe the tumor growth of each group,to compute and compare to the tumor inhibition rates.the pathological varieties of the tumor were observed.Results The tumor volumes were compared with each group after two weeks of radiotherapy.Compared with tumor contral group,pioglitazone treated group,radiotherapy group and radiotherapy combined with pioglitazone treated group could decrease the volume of tumor (P =0.008,P =0.001,P =0.001).Compared with pioglitazone treated group,the effect of radiotherapy group or radiotherapy combined with pioglitazone treated group was more evident (P =0.026,P =0.018).Contrasted with radiotherapy group,radiotherapy combined with pioglitazone treated group had no significant difference (P =0.335).The tumor inhibition rates of pioglitazone treated group,radiotherapy group and radiotherapy combined with pioglitazone treated group were 46.30 ％,68.60 ％ and 70.01％.The pathological varieties of tumor were obvious.Conclusion Pioglitazone,radiotherapy and radiotherapy combined with pioglitazone could inhibit the growth of tumor.PPARγ is a new target for tumor treatment.

Objective To observe the effect of enteral nutritional emulsion on nutritional status and immune function in patients with gastrointestinal cancer. Methods From January 2014 to January 2017,100 patients with gastrointestinal cancer in Yuncheng Central Hospital were selected in the research and randomly divided into two groups according to the digital table, with 50 cases in each group. All the patients underwent chemotherapy, the observation group was given enteral nutritional emulsion during chemotherapy. The indicators of nutritional status, immune function, the effect of chemotherapy, the incidence of adverse reactions were compared between the two groups. Results After treatment,the prealbumin,albumin,transferrin and hemoglobin levels in the observation group were (0. 30 ± 0. 07)g/ L,(32. 93 ± 1. 67)g/ L,(2. 61 ± 0. 15)g/ L,(109. 94 ± 2. 31)g/ L,respectively,which in the control group were (0. 19 ± 0. 09)g/ L,(30. 89 ± 1. 49)g/ L,(2. 49 ± 0. 14)g/ L,(108. 07 ± 2. 04)g/ L,respectively, there were statistically significant differences between the two groups(t = 6. 822,6. 445,4. 135,4. 291,all P < 0. 05). After treatment,the CD +3 ,CD4 / CD8,NK cells in the observation group were (43. 84 ± 7. 65)％ ,(1. 70 ± 0. 18) and (37. 78 ± 7. 51)％ ,respectively,which in the control group were (36. 12 ± 6. 57)％ ,(1. 52 ± 0. 19) and (31. 07 ± 4. 20)％ ,respectively,there were statistically significant differences between the two groups ( t = 5. 413,4. 863, 6. 124,all P < 0. 05). The objective response rate of the observation group (64％ ) was significantly higher than that of the control group(44％ )(χ2 = 4. 026,P < 0. 05). The incidence rate of adverse reaction of the observation group (18％ ) was significantly lower than that of the control group(36％ )(χ2 = 4. 110,P < 0. 05). Conclusion Enteral nutritional emulsion can effectively improve the nutritional status and immune function of patients with gastrointestinal cancer during chemotherapy,but also can effectively enhance sensitivity to chemotherapy,improve the effect of chemo-therapy,and the safety is reliable.

Objective To explore the value of tricuspid annular displacement (TAD) in evaluating right ventricular systolic function by speckle tracking imaging.Methods Forty healthy people (RVEF ＞50%) and forty five patients(RVEF ＜50%)were enrolled in this study.The apical four-chamber view was obtained and tricuspid annular midpoint displacement (TADmid) were measured by speckle tracking imaging.Right ventricular ejection fraction (RVEF) was measured by three-dimensional echocardiography.The correlation of tricuspid annular midpoint displacement (TADmid) and right ventricular ejection fraction (RVEF) was analyzed.Results These was statistical significance of tricuspid annular midpoint displacement between healthy people (RVEF ＞ 50%) and patients (RVEF ＜ 50%) (t = 7.28,P ＜ 0.01).There was positive correlation between TADmid and RVEF (P ＜ 0.01).The correlation coefficient between TADmid and RVEF was 0.76 (P ＜ 0.0l).The cut-off value of TADmid for RVEF ＞ 50% was 9.7 with a sensitivity of 72% and a specificity of 86%.Conclusions TAD by speckle tracking imaging showed an excellent correlation with RVEF.Tricuspid annular displacement by speckle tracking imaging was a simple and easy method,and it might be a new index in assessing right ventricular systolic function in clinical practice.

ObjectiveTo evaluate the frequency of antinucleosome antibody(AnuA) in juvenile systemic lupus erythematosus(JSLE),comparing it to that observed for anti-dsDNA,anti-Sm antibodies,and explore the correlation of these antibodies with clinical manifestations and disease activity.MethodsWe included 80 children with JSLE and 56 children with other rheumatic diseases into this study.Clinical records were reviewed.AnuA,antinuclear antibody (ANA) and anti-Sm antibody were detected by ELISA,ⅡF and Western-blot respectively.Anti-DNA were detected by ELISA and ⅡF.Disease activity was assessed by SLEDAI score.ResultsAnuA showed sensitivity of 76.25％ and specificity of 98.21％.AnuA combine with anti-dsDNA antibody or anti-Sm antibody was detected,the sensitivities and specificities were 83.05％,86.44％ and 96.43％,98.21％,respectively.It showed that the sensitivity was higher than any one of the three.The presence of AnuA was associated with red blood cell count,hemoglobin level,ESR,hematuria,low complement levels and anti-dsDNA antibody (r=-0.499,-0.503,0.388,0.227,0.303,0.531,P=0.000,0.000,0.000,0.042,0.006,0.000).The presence of AnuA was associated with the SLEDAI score(P=0.000). Conclusion AunA hasthe highest sensitivity and specificityamongthese autoabtibodies,particularly,when combined with anti-dsDNA antibody or anti-Sm antibody.The level of AnuA is associated with the disease activity of JSLE.AnuA is not only useful for the diagnosis of JSLE but also for evaluation of the disease activity.

Objective To observe the effect of selective intra-arterial thrombolytic therapy for central retinal artery thrombosis. Methods 100 000-250 000U of urokinase were injected into the ophthalmic artery via a catheter in the ophthalmic artery or the internal carotid artery to carry out the thrombolytic therapy. Results The visual acuity was significantly improved from light perception or finger counting to 0.1 in 8 of a total of 26 cases, partially improved in 10 cases, and no change in vision in 8 cases. The time interval from the onset of the disease to thrombolysis was within 8 days in the 18 patients who showed improvement, 3 of them showed significant improvement, of whom 2 received the treatment within 3 days. Conclusion Selective intra-arterial thrombolytic therapy is effective for the treatment of central retinal artery thrombosis. The effect was closely related to the time interval from the onset of the disease to thrombolysis. The thrombolytic therapy should be carried out as soon as possible.

Objective To preliminarily understand and evaluate the fertility ability of male infertility patients by analyzing the semen routine testing results to assist further clinical diagnosis and treatment .Methods 7 586 cases of semen routine detection re‐sults were performed the retrospective analysis and statistics by classification according to the indexes of semen volume ,pH value , liquefaction time ,sperm density ,sperm activity and sperm deformation rate .Results Among 7 586 cases of semen routine examina‐tion ,there were 1 450 cases(19 .1% ) of semen volume less than 2 .0 mL ;1 372 cases (18 .1% ) of pH < 7 .2 ;2 740 cases(36 .1% ) of liquefaction time > 60 min ;989 cases(13 .0% ) of sperm density lower than 20 × 106 /mL ;3 719 cases(49 .0% ) of sperm activity grade a + b less than 50% ;3 431 cases(45 .2% ) of sperm deformation rate less than 15% ;141 cases(1 .9% ) of aspermatism ;1 119 cases (14 .8% ) had no abnormality in each index .Conclusion Semen liquefaction ,sperm motility and sperm deformation rate are the indexes with the highest abnormal rate in the semen routine detection ;the semen routine detection provides the important exper‐imental data for clinical diagnosis and treatment of male infertility ,the objective and correct analysis of the detection result is of great significance to the judgment of male fertility .

The aim of the study is to investigate the effect of nardosinone (Nar) on neuronal injury induced by oxygen-glucose deprivation (OGD) in primary cortical cultures isolated from embryos at gestational day 14. MTT method was used to determine the dosage regimen of Nar in primary neuronal cultures and observe the influence of Nar on the neurons suffering OGD; Western blotting analysis was used to detect expressions of protein kinase A (PKA), Ras related protein 1 (Rap1), mitogen-activated protein kinase kinase 1 (MEK1) and phospho-extracellular signal-regulated kinase 1/2 (p-ERK1/2) of OGD-injured or uninjured primary cultured neurons after Nar treatment. Results showed that Nar (50 and 100 micromol x L(-1)) improved the cell viability during OGD damage (P < 0.01) and increased the expression of PKA, Rap1, MEK1 and p-ERK1/2 in injured neurons. Additionally, elevations of PKA, Rapl, MEK1 and p-ERK1/2 in uninjured neurons were caused by Nar (50, 100 and 200 micromol x L(-1)) with a dose-dependent tenclency as well (P < 0.01). In conclusion, Nar could protect against the neuronal injury exposed to OGD, which may be relevant to the promotion of PKA and ERK signaling pathway.

Objective To discuss the image characters and the technical manipulation of endovascular embolization for cerebral AVM with bleeding. Methods The cerebral AVMs with bleeding in 56 cases were confirmed by CT, MRI, and whole cerebral DSA. Depended on the nidus of AVM, the superselective endovascular embolization with NBCA or embolization combined with radiological surgery was chosen. Results The nidus was eliminated for 100% in 36 cases after embolization for 1 to 3 processes. The rebleeding was found in 2 cases with new growth and survival aneurysm in nidus during the follow-up period and treatment with X-knife, and was cured by the second embolization. Conclusion The main causes of AVM bleeding included aneurysm and aneurysm-like dilation beside and located at the nidus, fine draining veins, and growth in the ventricles. To prevent the brain from bleeding, it is favourable to eliminate the aneurysm in AVM during embolization.

Objective To monitor the performance constancy of the HS-126E automatic X-ray film processor and to investigate the effects of chemical processing time on the reproducibility of film dosimetry.Methods With films having identical exposure,15 ready-pack Kodak X-omat-V films from the same batch were divided into 5 groups and exposed one by one under the identical conditions.One group of test film was processed per day from Monday to Friday with identical temperature and time.All films were digitized with a Vidar VXR-16 bit film digitizer. The average optical density(A) at irradiation field center was analyzed with RIT113 film dosimetry system software.At the same time,another 10 films in 5 groups from the same batch were exposed with vertical method and processed to get the calibration data and sensitometric curves.The film sensitivities,i.e.,ratio of A to delivered dose,were calculated and compared between groups.Another 15 films in 5 groups were exposed with horizontal method and processed similarly to the test films,the point dose at 1.5?cm depth on the central axis was calculated with sensitometric curve and compared with the value measured by ion chamber.Results Variations of A value in each group was less than 2%.During the processing period,a trend of decrease along with time in mean A value decreased day by day was observed.Both the standard deviations and coefficients of variation in all groups were within 2%.One-way ANOVA showed that there was a significant difference of the mean A value among all films from Monday to Friday(P

OBJECTIVE To investigate the clinical distribution and drug resistance of clinical Pseudomonas aerugilosa isolates,and offer reasonable experimental data for clinical therapy.METHODS P.aerugilosa was identified by ATB Expression system,and its drug resistance was determined by Kirby-bauer and ATB Expression method.RESULTS The main departments in which frequently P.aerugilosa infection accurred were Intensive Care Unit(41.2%) and Respiration Departments(19.3%).The common site of P.aerugilosa infection was respiratory tract(68.2%).The sensitive rate of P.aerugilosa to polymyxin E and cefoperazone/sulbactam was the highest(95.1% and 91.4%),while to meropenem and imipenem was 77.5% and 70.6%.The highest resistant rate of P.aerugilosa to trimethoprim/sulfamethoxazole,ampicillin/sulbactam was 97.1% and 95.1%.The resistunce to ciprofloxacin,ticarcillin and piperacillin,were 64.9%,63.3% and 56.3%.CONCLUSIONS P.aeruginosa is major pathoge in our hospital.It is important to select antibiotics correctly according to the results of susceptibility tests.