OBJECTIVETo amplify mouse prostate stem cell antigen (mPSCA) gene and construct a recombinant plasmid to obtain mPSCA protein and identify its antigenicity.

METHODSThe gene of mPSCA was amplified by RT-PCR from mouse prostate cancer cell line RM-1 with the signal peptide sequence removed. The PCR product was cloned into pET-42a prokaryotic expression vector to construct the recombinant plasmid pET-42a-mPSCA, which was transformed into BL21 (DE3) for mPSCA expression. The fusion protein was purified and identified by SDS-PAGE and Western blotting. The antigenicity of the purified protein was characterized by ELISA.

RESULTSThe mPSCA gene was obtained with an identical sequence to that retrieved in GenBank. The prokaryotic expression vector for mPSCA was successfully constructed as confirmed by enzyme digestion and DNA sequencing. Both Western blotting and ELISA demonstrated the antigenicity of the purified mPSCA protein.

CONCLUSIONThe purified mPSCA obtained possesses good antigenicity, which will facilitate further study of immunotherapy for prostate cancer targeting PSCA.

Animals ; Antigens, Neoplasm ; genetics ; immunology ; isolation & purification ; Cloning, Molecular ; Escherichia coli ; metabolism ; GPI-Linked Proteins ; genetics ; immunology ; isolation & purification ; Genetic Vectors ; Male ; Mice ; Neoplasm Proteins ; genetics ; immunology ; isolation & purification ; Plasmids ; Prostate ; cytology

Objective To monitor the quality of intravenous infusions in a constructed closed-loop tracking system,and to discuss its application effect.Methods The information program is reinvested in a team for the closed-loop tracking system of the intravenous infusion.There are modules of processing medical orders,pharmacy preparations,execution procedures with the doctor's orders,as well as the computing module.The system was put into clinical trial operation in November 2021 and officially applied in January 2022.Specifically,the number of infusion-related adverse events/red-light alert is recorded and compared before(January to December 2021)and after(January to December 2022)the application of the system.Random sampling method has been performed in a ward to investigate the amount of abnormal infusion for the comparison.Results It is shown that it occurred 3 times for the infusion-related adverse events before the operation of the system,and a time after that.In the neurological inpatients,we observe the amount of red-light alert as 5,120.25±775.82 before the system operation,and 1,518.25±74.77 after that.It is shown to decline by 70.35％on average with statistical significance on the total difference(P＜0.001).Monthly amount of infusion in the ward is 5,184 with 155 times of overfast(2.99％)for 775 minutes in total,and with 207 times of slow(3.99％)for 1,035 minutes in total.The average handling time per contact is 5 minutes.Conclusion The closed-loop tracking system for intravenous infusion quality monitoring can track and record the whole process of intravenous infusions.It is expected to provide accurate and objective data for closed-loop quality inspection on clinical nursing practice,which is conducive to nursing managers for any potential problems during infusion processing timely,to promote standardized nursing operations,and to further improve the safety of inpatients with intravenous infusion and medication.

Objective:To analyze the impact of multi-color non-woven packaging in surgical instrument management on surgical turnover efficiency,surgical instrument preparation,and satisfaction of surgical medical staff and patients.Methods:31,589 surgical instruments from Beijing Friendship Hospital Capital Medical University from March 2016 to December 2021 were Selected.According to different management methods,71,007 surgical patients and corresponding 16,224 surgical instruments from March 2016 to January 2019 were included in the control group,which adopted traditional gauze-cotton bag packaging surgical instrument management method;62,548 surgical patients and the corresponding 15,365 surgical instruments from February 2019 to December 2021 were included in the observation group,which adopted multi-color non-woven packaging surgical instrument management method.The surgical turnover efficiency,surgical instrument preparation and satisfaction of surgical staff and patients were compared between the two groups.Results:The room entry time,room entry to skin incision time,the following surgery room entry to skin incision time,and surgical delay rate of surgeries of patients in the observation group were lower than those in the control group,the difference was statistically significant(t=52.681,37.167,72.524,x2=9.605,P<0.05).Compared with the control group,the number of outgoing deliveries,assembly errors and loss of instruments decreased significantly,the number of intact devices was increased significantly,the difference was statistically significant(x2=107.345,11.782,8.727,66.223,P<0.05.The satisfaction of patients and medical staff in the observation group was significantly higher than that in the control group,the difference was statistically significant(t=100.723,6.756,P<0.05).Conclusion:The application of multi-color non-woven packaging in the management of surgical instruments can help to improve surgical turnover rates,improve the efficiency of the central sterile supply department by reducing the number of outgoing deliveries,assembly errors and instrument losses,and improve the satisfaction of surgical medical staff and patients with multi-color non-woven packaging of surgical instruments.