Objective To analyze the clinical application of intravenous nutrition liquid combined with psychological intervention in the treatment of the patients with hyperemesis gravidarum. Methods From July 2014 to March 2017, 162 cases according to the wishes were divided into the control group and the study group, 81 cases in each group. The control group were given nutrient solution by intravenous and the routine nursing, the study group i were received psychological intervention on the basis of the treatment in the control group. The treatment in the two groups were compared. Results The total effective rate was 95.06%(77/81) in the study group, and was 77.78%(63/81) in the control group. The difference in the two groups had statistically significant (P<0.01). The average hospitalization day was (5.3±0.6)d in the study group, while (7.4±0.4)d in the control group, the difference was statistically significant between the two groups(t=6.42, P<0.01). Conclusion Intravenous nutrition combined with psychological intervention in the treatment of hyperemesis gravidarum has good effect, which can effectively improve the clinical treatment effect, and reduce the hospitalization day, and has high clinical application value.

Objective To investigate psychological intervention on the treatment of the patients with rivanol induced labor in late pregnancy with depressive.Methods From March 2014 to February 2017 in Shaoxing central hospital, 54 cases with late pregnancy as the research object, who were induced labor to terminate pregnancy with rivanol because severe fetal deformity.According to the wishes of this patients, they were randomly divided into the control group and the observation group, 27 cases in each group.The control group were received conventional nursing, at this basis, the observation group were given psychological intervention.Hamilton Depression Scale(HAMD) score in the two groups were observed and compared before and after nursing intervention.Results After nursing intervention, the depression scores were decreased significantly(P<0.05), and the depression scores in the observation group decreased significantly than that in the control group(P<0.05).Conclusion The psychological nursing intervention for patients in late pregnancy with depression Noel induced labor in late pregnancy who were induced labor to terminate pregnancy with rivanol has important significance in the peri operative treatment, which can effectively reduce the negative emotions, improve the prognosis, and is worthy of clinical application.

Objectives To develop a multi-stage and multi-epitope vaccine, which consists of epitopes from the early secretory and latency-associated antigens of Mycobacterium tuberculosis (MTB). Methods The B-cell, cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes of 12 proteins were predicted using an immunoinformatics. The epitopes with antigenicity, without cytotoxicity and sensitization, were further screened to construct the multi-epitope vaccine. Furthermore, the proposed vaccine underwent physicochemical properties analysis and secondary structure prediction as well as 3D structure modeling, refinement and validation. Then the refined model was docked with TLR4. Finally, an immune simulation of the vaccine was carried out. Results The proposed vaccine, which consists of 12 B-cell, 11 CTL and 12 HTL epitopes, had a flexible and stable globular conformation as well as a thermostable and hydrophilic structure. A stable interaction of the vaccine with TLR4 was confirmed by molecular docking. The efficiency of the candidate vaccine to trigger effective cellular and humoral immune responses was assessed by immune simulation. Conclusion A multi-stage multi-epitope MTB vaccine construction strategy based on immunoinformatics is proposed, which is expected to prevent both active and latent MTB infection.
Mycobacterium tuberculosis/metabolism* ; Molecular Docking Simulation ; Toll-Like Receptor 4 ; Epitopes, T-Lymphocyte/chemistry* ; Epitopes, B-Lymphocyte/chemistry* ; Vaccines, Subunit/chemistry* ; Computational Biology/methods*

【Objective】 To investigate the unqualified rate of anti-HIV detection of blood screening laboratories in Beijing-Tianjin-Hebei region, and explore the differences in anti-HIV detection ability and influencing factors in each laboratory. 【Methods】 Through filling questionnaires via e-mail, the anti-HIV ELISA unqualified rate and confirmed (WB) positive results (data) from January to December 2018 from 15 blood screening laboratories in Beijing-Tianjin-Hebei region were collected. Our laboratory was responsible for data collection and confirmation, and statistics software SPSS22.0 was used for analysis. 【Results】 1) There was a statistically significant difference among the unqualified rate of anti-HIV ELISA(6.77‱~35.71‱) and confirmed positive rate(0.60‱~3.56‱) in 15 blood screening laboratories in Beijing-Tianjin-Hebei region (P<0.05); 2) There were significant differencse among the ELISA unqualified rate and the confirmed positive rate of 8 reagents for anti-HIV detection(P<0.01), and the sensitivity of the 4th generation detection reagent and the imported reagent was higher than that of the 3rd generation reagent and the domestic reagent. The anti-HIV ELISA unqualified rate of R5 was the highest (19.08‱). 3)There were significant differences in the anti-HIV ELISA unqualified rate of R1, R2, R3, R5 and R7 reagents among different blood station laboratories(P<0.05), and there were no significant differences in the anti-HIV ELISA unqualified rate of R4, R6 and R8 reagents among different blood station laboratories(P>0.05). 4)The unqualified rate of anti-HIV ELISA of laboratories using different regents showed significant differences(P<0.05), except H, J, M. The unqualified rate of imported reagent was significantly higher than that of domestic reagents of laboratories using imported and domestic reagents combinations(P<0.05), except O. 62.5% (5/8) laboratories using domestic 3^rd and 4^th generation reagent combination showed significant differences in the unqualified rates among different reagents(P<0.05); 5) The positive rate of single-reagent(62.02%~95.45%)in 15 blood screening laboratories showed significant difference(P<0.001), and A was the lowest (62.02%). 【Conclusion】 The anti-HIV detection ability among 15 blood screening laboratories in Beijing-Tianjin-Hebei region is quite different. The application of different reagents is the main factor for the difference, and other factors such as personnel, instruments and test strategies also has a great impact on the detection of anti-HIV. It is still necessary to promote the process of homogenization of blood testing quality among blood screening laboratories in Beijing-Tianjin-Hebei region.