Premature ventricular complex (PVC)-induced cardiomyopathy is due to frequent PVCs and it can be potentially reversible in selected patients resolves after catheter ablation.PVCs burden is importantly associated with impaired left ventricular(LV) function.Necessary evaluation is important for PVCs patients.For patients with high PVC burdens above 15％-25％ of the total cardiac beats in 24 hours,medical treatment should be used to reduce PVC burden and to improve LV function.Catheter ablation of PVCs is recommended for selected patients with high PVC burdens associated with a decline in LV systolic function.

Bilingual teaching is very important to the development of medical education and integration with the advance medicine knowledge in the world.Rich teaching experiences have been summarized during these years.This article summarizes the bilingual teaching experience from four parts including the concept of bilingual education,conditions of bilingual education,methods of bilingual education,results of bilingual education.We have also found some problems during teaching and worked out the improvement methods.

Laiminin,which has been found 15 isoforms up to today,is a kind of noncollagen glycoprotein of extracellular matrix.However,the functions of laminin varis from each other,which mainly takes participation in the process of the cell adherence,migration,differentiation,growth,cell signaling and tissue repair in vivo.In recent years,some scholars have found that laminin plays an important role in angiogenesis.The purpose of this article is to summarize the role of laminin in angiogensis and sum up the current research progress.

ObjectiveTo evaluate clinical value of evidence-based nursing intervention in treatment of elderly patients with cardiovascular interventional surgery during perioperative period. Methods 150 cases of elderly patients with cardiovascular diseases who underwent interventional surgical treatment were selected.They were randomly divided into the observation group(80 cases)and the control group(70 patients). ResultsThe occurrence and degree of discomfort of bedridden patients in the observation group were milder,and the complications due to use of anticoagulants and pressing dressing were significantly less than the control group.The satisfaction degree and mastering of disease related knowledge in the observation group were significantly better than that of the control group. ConclusionsApplication of evidence-based nursing intervention during perioperative period for elderly patients with cardiovascular interventional surgery can reduce discomfort and complications of patients,as well as improve satisfaction degree and mastering of disease-related knowledge of patients and their families,which is worthy of promotion.

Objective To test the reliability and validity of the Chinese version STRATIFY (St Thomas Risk Assessment Tool in Falling Elderly Inpatients) for geriatric inpatients.Methods The scale was translated and 404 geriatric inpatients were evaluated by the Chinese version STRATIFY and evaluate its reliability and validity.Results Chinese version STRATIFY was found with good feasibility.The Pearson correlation coefficient of reliability between testers was 0.951,the retest reliability was 0.885,internal consistency reliability (Cronbach's αt) was 0.523.The values of item-total correlation was over 0.3.The distinguish validity in difference of STRATIFY scores between groups with/without fall history was statistically significant.Predictive validity was moderate,the best cut-off point was determined at 2 points,and sensitivity and specificity were respectively 64.3％ and 78.2％.Conclusions Chinese version STRATIFY applied in Chinese geriatric inpatients showed good reliability between testers,retest reliability,distinguish validity and predictive validity,and it could be used to assess the fall risk of Chinese geriatric inpatients.In addition,as the internal consistency was lower,we can revise the entry of STRATIFY to provide a more accurate predicting tool.

Objective To study CTLA4Ig gene expressed in dermal papilla cells and to provide evidence for immune tolerance after dermal papilla cells transplantation. Methods CTLA4Ig cDNA was transferred into dermal papilla cells by recombinant adenovirus vector, and the dermal papilla cells containing CTLA4Ig gene were transplanted into mice skin. The target gene expression was detected by histological and immunohistochemistry technique. Results CTLA4Ig protein was expressed in plasma 6 hours after gene transfection and increased gradually. When the transferred papilla cells were transplanted into mice skin the gene began to express in 24 hours and lasted for 2 weeks. No rejection was observed. Conclusion Dermal papilla cells containing CTLA4Ig gene can survive in vitro and in vivo and express CTLA4Ig for a long time.

Objective To study the relationship between the apolipoprotein B(apo B) gene polymorphism and mycoardial infarction.Methods Two polymorphic sites of the apo B gene,Xba1 and Msp1 were examined by PCR in a sample of 65 patients with myocardial infarction(MI) and 60 healthy individuals selected from a population of the chinese Han nationality.Results The frequency of X+allele(presence of Xba1 cutting site)in MI cases was significantly higher than that in controls(P

Objective:To study the effect of seeding cells of tissue-engineered skins,keratinocytes and fibroblasts,on the phenotypes of langerhans cells (LC).Methods:Peripheral blood mononuclear cells were induced by cytokines,granulocyte macrophage-colony stimulating factor (GM-CSF),interleukin 4 (IL-4),transforming growth factor β1 (TGF-β1) to generate LCs,which were subsequently cocultured with allogenic keratinocytes or fibroblasts.Then the phenotypes of langerhans cells was detected by flow cytometer (FCM),and the degree of proliferation of autogeneic lymphocytes was assessed after stimulation with LCs.Results:Cultured LCs had low level expression of HLA-DR,CD80,CD86 and no expression of CD83.Cocultured with allogenic keratinocytes or fibroblasts,phenotypes of LCs did not change significantly.Simultaneously,LCs could not stimulate autogeneic lymphocytes proliferation.Conclusion:These data indicate that LCs cocultured with seeding cells of tissue-engineered skins remain immature state.It is suggested that seeding cells of tissue-engineered skins have low immunogenicity and are unable to induce significant immunological rejection of host after transplantation.

Objective To observe the efficacy, safety and feasibility of the four kinds of anticoagulation method in hemodialysis patients with high risk of hemorrhage. Methods 128 patients with high risk of hemorrhage who had undergone hemodialysis were randomly divided into four groups. In group A, 68patients were resorted with 30% regional sodium citrate anticoagulation(RCA). In group B, 60 patients were treated with local heparin anticoagulation. In group C, 64 patients were treated with low molecular weight heparin (LMWH). In group D, 66 patients received no heparinization hemodialysis. The changes of bleeding, clotting function, adverse effect and nursing strategies were studied. Results All patients of group A completed regular hemodialysis with satisfactory indices, 6 cases with bleeding and primary bleeding aggravated in group B, 8 cases with bleeding and primary bleeding aggravated in group C, 8 cases occurred grade three coagulation and finished dialysis in group D. 3 cases in group A and 2 cases in group B occurred adverse reaction, but no adverse reaction was seen in group C and D. Conclusions RCA is safe,effective and can be easily handled in regular hemodialysis. It is an ideal dialysate for hemodialysis in patients with high risk of hemorrhage.

45 cases of childhood extrinsic asthma (CEA) were divided into the treatment group with carboxymethykited starch (CMS) (n=30) and the control group (n=15). The total effective rate of the former was 63% (P