Objective To establish a method for the determination of Baicalin content in Shengjijiedu Granule by HPLC.Method HPLC was performed to determine naringin content on C18 column.The mobile phase consisted of a mixture of CH3OH-H2O-H3PO4(50 ∶50 ∶0.2) and detection wavelength was at 280 nm.Result The linearity of baicalin was in the range of 0.015～0.121 ?g (r=0.9999) and the average recovery rate was 100.98 %,RSD=0.84 %.Conclusion This method was simple,sensitive and accurate,and can be used for the quality control of Shengjijiedu granule.

Objective To detect the clinical factors related with liver metastasis in young patients with rectal cancer.Methods Three hundred and fifty young patients with rectal cancer were collected to set up the database.Single and multi-factor Logistic regression was applied to indicate the independent factors relating to liver metastasis.The regression equation to predict probability of liver metastasis from rectal cancer was established.Results Liver metastasis was 120 cases (34.3%).Single-factor analysis revealed that patho-organization type,pathologytype,infiltration extent,blood vessel invasion (BVI),TNM stage,operation character,the preoperative level of carcino-embryonic antigen,histology grading were related with liver metastasis.Multi-factor analysis revealed that only BVI (P=0.001),TNM stage (P=0.001),pathoorganization type (P=0.005),the preoperative level of CEA (P=0.008) and operation character (P=0.032) were independent factors to predict probability of liver metastasis.Conclusions Rectal cancer of young patients who being with BVI,advanced phase,high preoperative level of CEA,radical operation or poor differentiation degree,are apt to develop liver metastasis.They should be given further individualized intensive adjuvant treatment.