Objective:To analyse the whole DNA methylation status , expression of DNMT1 and DNA methylation status of DNMT1 promoter from patients with systemic lupus erythematosus ( SLE) .To assess the negative feedback effection of DNA methylation on the expression of DNMT1 in the patients with SLE.Methods:The whole DNA methylation status in T cells from 34 SLE patients and 23 healthy controls was assessed by the specific monoclonal antibodies to 5-methylcytosine (5-mc) and the DNA methylation status of DNMT1 promoter was assessed by the Bisulfite Conversion Kit and sequencing.Real time RT-PCR was applied to analyse DNMT 1 mRNA levels in T cells from the patients and controls.Results: SLE patients had siginificantly whole DNA hypomethylation than controls(P<0.05),and the whole DNA methylation was negative correlated with the SLE-DAI.There was no difference in levels of DNA methylation of DNMT1 promoter between patients and controls ,also no correlation between the levels of DNA methylation of DNMT 1 promoter and DNMT1 mRNA.Conclusion:There was no negative feedback effection of DNA methylation on the expression of DNMT 1 in the patients with SLE.The mechanism of lower expression of DNMT 1 in patients with SLE should be studied further.

OBJECTIVE: To study whether the therapeutic action of compound glycyrrhizin(SNMC) on allergic disorders can be achieved through inhibition on the expression of chemotactic factors in peripheral blood mononuclear cells(PBMC).METHODS: PBMC of 15 healthy subjects were obtained and divided into 4 drug-treated groups: SNMC group,dexamethasone group,mizolastine group,and loratadine group;meanwhile,a positive control group(proinflammatory stimulant was added) and a negative control group(no proinflammatory stimulant was added) were used as control.Fluorescent quantitation polymerase chain reaction was employed to determine the mRNA expression of the chemotatic factors including RANTES,MCP-1,MIP-1? and ELISA was employed to detect their concentrations in supernatant.RESULTS: In the positive control group,both the mRNA expressions and the concentrations of the 3 chemotatic factors increased significantly,and which were significantly lower in the drug-treatment groups than in the positive control group.There were significant differences between the positive control group and dexamethasone-treated group or SNMC-treated group in the expression of MIP-1? mRNA(P

Chronic urticaria is a very common allergic disease in the outpatient.It has complicated pathogenesis and there is no conclusion up to now.The activation of the mast cells and the release of the histamine are the key point of the chronic urticaria onset.In recent years,the studies have demonstrated that the autoimmunity and the chronic infection are the main cause of the disease.There are other factors including dysfunction of the coagulation,deficiency of the vitamin D,pseudoallergen,psychic factor and genetic mechanism.This article will make a review of the pathogenesis of the chronic urticaria from the aspects mentioned above.

Objective To investigate the effect of continuous nursing on the psychological status and quality of life in patients with low rectal cancer after Miles operation.Methods A total of 84 patients with low rectal carcinoma underwent Miles operation were selected from August 2014 to August 2016 in Henan Rongjun Hospital.The patients were divided into observation group (n =38) and control group (n =46) according to nursing measures.The patients in the control group were given routine nursing measures,while the patients in the observation group were given continuous nursing measures on the basis of routine nursing care.The psychological status and quality of life of the patients were compared between the two groups before and after intervention.Results There was no significant difference in swlf-rating anxiety scale (SAS) and self-rating depression scale(SDS) scores between the two groups before intervention (P ＞ 0.05).After six months intervention,the SAS and SDS scores in the two groups were significantly lower than those before intervention,and the scores of SAS and SDS in the observation group were significantly lower than those in the control group (P ＜0.05).There was no significant difference in the quality of life between the two groups before intervention (P ＞ 0.05).After six months intervention,the quality of life of patients in the two groups were significantly higher than those before intervention,and the quality of life of patients in the observation group were significantly higher than those in the control group (P ＜ 0.05).Conclusion Continuous nursing can significantly improve the negative emotions of anxiety and depression,and improve the quality of life in patients with low rectal cancer after Miles operation.

Objective To investigate the effect of the use of audio and video feedback on the rehabilitation of patients with ankylosing spondylitis (AS). Methods 60 AS patients were divided into two parts (32 in the experimental group and 28 in the control group) by random number table method, and two groups of patients were treated with the same drug treatment. Patients in the control group were instructed by oral instruction, in the experimental group, the use of audio and video feedback teaching guide patients family exercise, compared with the two groups of patients with four months of follow-up, family exercise compliance, joint function and quality of life, and other indicators. Results Four months after the intervention, the experimental group patients family exercise compliance was better than 84%(27/32), higher than the control group of 36% (10/28), the difference was statistically significant (χ2=9.238, P < 0.01). Patients in the experimental group in four months after the intervention, the health questionnaire to investigate the physiological function, role limitation due to physical problems, body pain, general health, vitality, social function, role limitation due to emotional problems, mental health eight dimension scores respectively were (72.7±8.4), (81.5±9.5), (57.2±8.4), (65.5±8.4), (62.9±7.2), (78.4±7.4), (67.3±6.2), (77.2±7.6) points while in the control group were (60.8±9.1), (72.1±9.6), (50.3±9.4), (60.8±7.0), (58.2±9.1), (74.4±8.7), (60.2±7.2), (74.3±7.4) points, the difference between the two groups had statistical significance (t=1.493-5.266, P < 0.05 or 0.01). Four months after the intervention, the scores of Bath Ankylosing Spondylitis Functional Index (BASFI) of patients with AS in the experimental group were (1.1± 0.8), (1.7±0.2), (1.8±1.3), (1.6±1.0), (3.1±2.1), (1.0±0.6), (1.9±1.6), (3.8±2.5), (2.6±1.7), (2.7±1.6) points, while (3.8±1.6), (2.8±1.5),(2.6±1.5)、(2.8±1.2),(4.4±2.2),(2.2±1.0),(3.3±1.6),(6.5±2.5),(5.6±2.6), (4.3 ± 1.5)control group, the two groups the difference was statistically significant (t=0.282-8.427, P<0.05). Conclusions Application of audio and video feedback teaching in patients with AS rehabilitation guidance, help patients to master the family training methods, improve the joint function of patients, improve the quality of life.

Diffuse large B-cell lymphoma (DLBCL) is the most common type of non-Hodgkin lymphoma with highly heterogeneous both clinically and biologically. So the prediction for prognosis of DLBCL and identifying patients with high risk are coming into the focus of the 58th American Society of Hematology (ASH) Annual Meeting. International prognostic index (IPI) risk model is widely known as the powerful prognostic indicator for DLBCL but in rituximab-era is challenged by a variety of new prognosis systems. Biological markers and mutation and expression of genes play an important role in the pathogenesis and progression of lymphoma, and its prognostic value draw much more attention. The prognostic factors of DLBCL, specific types, such as primary breast DLBCL, intravascular large B-cell lymphoma, the presence of a DLBCL component in a follicular lymphoma and relapsed/refractory DLBCL have been paid more attention in this meeting.

20/min,PaCO2 12?109 or 0.10.26 patients with sepsis infected by Gram negative bacilli were categorized as G-group,15 infected by Gram positive coccus as G+ group and 15 healthy persons as control group.All the cases were proved to have infection by body fluid examination under microscope or bacterial culture.The level of flagellin was determined by ELISA.The peripheral blood mononuclear cells(PBMC)were isolated by the density gradient centrifugation,and total RNA of PBMCs was extracted by RNA BIOzol extract.The level of TLR5 mRNA in the peripheral blood was detected by semi-quantitative RT-PCR.The statistic significance among groups was examined by Students' t test.Results Plasma flagellin levels were below the detection limit of the ELISA kit in both G+ and control groups,while the mean level of plasma flagellin amounted to 6.636?5.147ng/ml in G-group.TLR5 mRNA expression in G-group was significantly higher than that in G+ and control groups,and no significant difference was found between G+ group and control group.Conclusion Flagellin may be involved in the development of ALI through interacts with TLR5.

0.05).It is concluded that furazolidone in small doses is as efficacious as Denol in the treatment of duodenal ulcer and the clearance of H.pylori,and it be a safe substitute for the routine dose of furazolidone for the treatment of duodenal ulcer.

Aim To investigate effects of moxonidine on hemodynamics in anesthetized rats. Methods The indexes of hemodynamics were examined by catheterization of arteries in anesthetized Wistar rats. Results DBP, +dp/dt_ max, -dp/dt_ max,t-dp/dt_ max ,V_ pm and V_ max were significantly declined in dose-dependent manner after administration, but HR and SBP didn't change significantly. Conclusion These results showed that antihypertensive effects of moxonidine were related to inhibition of systolic and diastolic functions of myocardium.Furthermore,the antihypertensive effects of moxonidine were associated with reduction of peripheral vascular resistance.

Objective To investigate the expressions of miR-17 and miR-20a in CD4+ T cells from patients with systemic lupus erythematosus (SLE) and their correlations with disease activity.Methods CD4+ T cells were isolated from the venous blood of 30 patients with SLE and 18 healthy human controls.RNA was extracted from the CD4+T cells,and real-time fluorescence-based quantitative PCR (qPCR) was performed to determine the expression levels of miR-17 and miR-20a.The intergroup comparison of miR-17 and miR-20a expression levels was done using t test and Mann-Whitney test,and the correlations of miR-17 and miR-20a expressions with clinical parameters were assessed using Pearson or Spearman correlation coefficients.Results The patients with SLE showed increased expression levels (2-△c~) of miR-17 and miR-20a compared with the healthy controls (0.24 ± 0.08 vs.0.15 ± 0.06 for miR-17,0.19 ± 0.10 vs.0.12 ± 0.09 for miR-20a,both P ＜ 0.05).The miR-17 and miR-20a expression levels were also significantly higher in patients with active SLE than in those with inactive SLE and the healthy controls (0.27 ± 0.07 vs.0.20 ± 0.05 and 0.16 ± 0.08 for miR-17,0.22 ± 0.10 vs.0.15-0.07 and 0.13 ± 0.09 for miR-20a,all P ＜ 0.05),but similar between the patients with inactive SLE and healthy controls (both P ＞ 0.05).The expression levels of both miR-17 and miR-20a were positively correlated with SLE disease activity index (SLEDAI)(r =0.45,0.38,respectively,both P ＜ 0.05) and anti-dsDNA antibody level (r =0.54,0.46,respectively,both P ＜0.05),but negatively correlated with serum C3 levels (r =-0.43,-0.42,respectively,both P ＜ 0.05).Condusions The expressions of miR-17 and miR-20a are increased in CD4+ T cells from patients with SLE,and correlated with the disease activity in SLE.