Objective To study the value of 3D-CT reconstruction in the diagnosis of surface involved lesions of bone for its nature and location,comparing with plain film and 2D-CT.Methods Surface-involved lesions of bone revealed by plain-film were performed spiral CT examination.Multi-planar reconstruction(MPR)and surface shading display(SSD)were performed among 20 cases which had pathologic results.The diagnosing correct rate of nature and location in all the techniques were compared.Results The correct rate of nature diagnosing of plain film,2D-CT,MPR and SSD was 75%,80%,65%,60% respectively;correct rate of location diagnosing was 90%,75%,85%,95% respectively.The highest nature diagnostic correct rate was found in 2D-CT.The highest location diagnostic correct rate was found in SSD.Conclusion Spiral CT stereoscopic reconstruction is of great value in the location diagnosing of surface-involved bone lesion.

Objective To investigate the relationship between childhood obesity,asthma and lung function.Method Based on body weight and whether with asthma the 45 children were divided into normal control group,asthma group,obesity and asthma group,15 cases each.Each child was measured leptin levels and pulmonary function was measured with a spirometer.Each asthmatic children was surveyed by Asthma Control Test (C-ACT) questions.Results Asthmatic children have varying degrees harm of lung function.Obesity and asthma group compared with asthma group of children is more decreased in lung function.Children with asthma compared with normal control group of children is more higher in serum leptin concentration.Obesity and asthma children is highest in serum leptin concentrations.Asthmatic children C-ACT average score than obesity and asthma score,the difference was statistically significant (P ＜ 0.01).Conclusion Lung function and leptin abnormalities are exist in children with asthma.Also in the case of asthma,the abnormal levels of lung function and leptin in obese children compared with normal-weight children are more apparent,C-ACT score are more lower,and asthma is more severe.Weight control in obese children with asthma is favorable measures to control asthma attacks.

Diabetes mellitus has become the third threaten diseases second only to cardiovascular disease and tumor.Many re-searches demonstrated that the microRNAs(miRNAs)in blood related to the occurrence and development of diabetes melli-tus.Because of the non-invasion and repeatability of circulating miRNAs,the circulating miRNAs have a broad application prospect of diagnosis and prognosis evaluation for various types of diabetes.This overview arms to introduce the source and characters of circulating miRNA,and also the progress of circulating miRNA related to diabetes mellitus,so as to lay a foun-dation for further research.

Objective To investigate the mechanisms and clinical characteristics of fatal asthma attack induced by amiodarone in the elderly,and to discuss its serious potent risks.Methods We reported two cases of fatal asthma attack in the elderly with fast atrial fibrillation induced by amiodarone,and discussed the clinical manifestation and emergency treatment,with literatures review.Results The two patients presented with respiratory arrest.After targeted therapies,including artificial ventilation and hormone injection,the patients were capable of spontaneously breathing,while still in severe asthma attack.And further anti-asthma treatment led to a complete remission.Conclusions Amiodarone may cause extremely severe asthma attack in elder patients with tachyarrhythmia complicated with bronchial hyper-responsiveness diseases such as asthma or chronic obstructive pulmonary diseases.Clinicians should pay great attention to this situation and be prepared to cope with serious adverse reactions.

Pancreatic cancer is a highly malignant tumor. Animal models of pancreatic cancer included xenograft models, carcinogen induced models, genetically engineered models. Xenograft models are relatively reliable and feasible, but the growth pattern is different between serve immunodeficienct animals and human beings. Carcinogens induced models simulated the environmental factors to reconstruct the development of pancreatic cancer. But carcinogens could have other biological effects. Genetically engineered models could make the occurrence of pancreatic cancer at the molecular level. But it is difficult to control the transgenic product accurately. No model could meet all the needs of different experiments. It is important to choose a suitable animal model in different experiments.

Aim To discover lead compound whose function is similar to EPO.Methods A high-throughput Fluorescent Polarization(FP) assay was established for screening ligand of EPO receptor from small molescules and natural products.In the FP assay,EPO receptor was abstracted from FVA cell.EPO was labeled with FITC.Results The optimized concentration of FITC-EPO was 10~(-9) mol?L~(-1).Different concentrations of DMSO(VDMSO/Vtotal: 0%,0.5%,1%,2.5%,5%) didn′t affect the FP assay.The value of Z′ in the FP assay was 0.78.Conclusion The assay was robust and of high sensitivity.

Objective To summarize the experience of single incision thoracoscopic surgery (SITS),and to explore its feasibility and safety for treating thoracic diseases.Methods The clinical data in 186 cases of SITS in our hospital from August 2014 to March 2016 were retrospectively analyzed.Local lesion resection was performed in 171 cases and pulmonary lobectomy in 15 cases.Results The average operation time in local lesion resection was 46(10-75)min and average blood loss amount was 23(5-65)mL;11 cases were converted to double holes during operation and 6 cases converted to three holes;the average postoperative hospitalization stay was 4.7(3-9)d.The average operation time in the cases of pulmonary lobectomy was 152(95-215)min and average blood loss amount was 96(60-195)mL;2 cases converted to double holes during operation and 2 cases converted to three holes during operation;the average postoperative hospitalization stay was 6.7(5-9)d.No perioperative death or severe complications were observed in all cases.Conclusion SITS for treating thoracic diseases is safe,reliable and beautiful with little complications,less trauma and faster recovery.

Objective To study the effect of small interfering RNA targeted on Apollon for proliferation on pancreatic cancer cells and its possible acting mechanism.Methods The small interfering RNA targeted on apollon in our previous study was transfected to the cells using LipofectamineTM 2000,after 48 hours transtection.The inhibitory effects of small interfering RNA targeted on Apollon (Apollon siRNA)on cell proliferation were detected by WST-8.Their inhibition rate and IC50 were calculated.The percentage of apoptosis cells were determined by flow cytometry.The expression of Apollon mRNA was analyzed by real time fluorescent quantitative reverse transcription polymerase chain reaction.The Apollon protein ex-pression levels were detected by western blotting.Results Apollon siRNA could effectively inhibit the proliferation of pan-creatic cancer cell.The amount of apoptotic cells increased significantly.The early apoptotic rate was 37.1%,and the RT-PCR results showed that the relative expression levels of Apollon mRNA were down-regulate,and shows a dose-effective-ness relations.The protein expression levels were decreased by Apollon siRNA.Conclusion Apollon siRNA can effectively inhibit the proliferation of pancreatic cancer cell.The mechanism may be work together to promote pancreative cancer cell early apoptosis and decreased the expression levels of gene and protein,which provides a novel potential approach for treat-ment of target therapy of pancreatic cancer.

Objective: To investigate the knowledge, attitudes and practice of AIDS control knowledge among students in a university in Hangzhou City, so as to provide the evidence for evaluating the effectiveness of AIDS control in universities. Methods: The knowledge, attitudes and practice towards AIDS control were investigated among students in a university in Hangzhou City using self-designed questionnaires in 2020, and were descriptively analyzed. Results: Totally 6 300 questionnaires were allocated, and 6 164 valid ones were recovered, with an effective recovery rate of 97.84%. The respondents included 2 837 male students ( 46.03% ) and 3 327 female students ( 53.97% ), and had a mean age of ( 18.20±0.76 ) years. The overall awareness of AIDS control knowledge was 73.49%. The awareness rates of “The rights of marriage, employment and school admission are protected among HIV-infected individuals by Chinese laws” ( 61.11% ) and “Homosexual contact in men is the current main transmission pattern among young students in China” ( 66.37% ) were relatively low. There were 768 university students with a history of sexual behaviors ( 12.46% ), and regular sex partners were the predominant sex partners at the first sexual contact ( 679 students, 90.78% ) and at the sexual behaviors during the past one year ( 402 students, 71.91% ). There were 515 students (67.14%) using condoms during the first sexual contact, and there were 300 ( 63.83% ) and 16 students ( 40.00% ) using condoms with regular and casual sex partners during the past one year, respectively. Among 20 men who had sex with men, only 5 students ( 25.00% ) used condoms at each sexual contact. Of students with sexual behaviors, there were 355 (46.22%) and 354 ( 46.09% ) students that considered to be infected with AIDS and other sex-transmitted diseases, and among the university students with sexual behaviors, there were 614 ( 79.95% ) students that were willing to receive HIV testing, and 409 ( 53.26% ) and 305 ( 39.71% ) students that were willing to receive HIV testing in hospitals and disease control and prevention institutions, respectively. Conclusions The awareness of AIDS control knowledge is low among students in the university in Hangzhou City, which does not reach the national goal of supervision and assessment for AIDS control in China. There are high-risk sexual behaviors and the rate of condom use is low.

Purpose To study the status of EGFR mutations and the expression of excision repair cross-complementation group 1 ( ER-CC1) and Ki-67 protein in patients with non-small cell lung cancer (NSCLC) and to examine the relationship between their expression and clinicopathologic features. Methods EGFR mutations were analyzed with DNA sequencing, and the expression of ERCC1 and Ki-67 protein was examined by immunohistochemistry EnVision. The relationship of EGFR mutations with the expression of ERCC1and Ki-67 and the clinicopathological features were analyzed. Results EGFR mutations were detected in 143 (143/291, 49. 1%) of the 291 specimens. EGFR mutations were found more frequently in women, non-smokers and adenocarcinoma. The difference of EGFR muta-tion rate between the histological subtypes according to the IASLC/ATS/ERS classification of lung adenocarcinoma was significantly ( P=0. 008). The mean tumor diameter was smaller in patients with EGFR mutations than in those with wild-type EGFR (P=0. 020). EGFR mutations were not related to age, lymph node metastasis. However, EGFR mutations were not related to the expression of ER-CC1 and Ki-67 protein (P>0. 050). Conclusions EGFR mutation is closely linked to several clinicopathological factors, such as gender, differentiation, and histological subtype. There is heterogeneity of EGFR mutation in patients with NSCLC. EGFR mutations were not related to the expression of ERCC1 and Ki-67 protein.