Objective To study and analyze the effect of fluoride faction shouted tons melitracen sheet (Deanxit)on abdominal pain symptoms of functional gastrointestinal disorders (FGIDs).Methods 82 patients with abdominal pain FGIDs were randomly divided into A group(n =40)and B group(n =42).A group was given conven-tional treatment,group B was given deanxit treatment on the basis of A group.The treatment efficacy and the total inci-dence of adverse reactions were analyzed.Results The total effective rate of group B (90.24%)was significantly higher than that in group A (65.85%),the difference was statistically significant (χ2 =4.538,P <0.05).There were no serious adverse reactions observed during treatment in the two groups,the incidence rate of adverse reactions between the two groups had no statistically significant difference (P >0.05).Conclusion Deanxit in the treatment of FGIDs can achieve satisfactory therapeutic effect,the drug can significantly alleviate the symptoms,andit is safe and without serious side effects.

Objective: To study the technological parameters of the purification process of total saponins of Rubus parvifolius L. with macroreticular resin. Methods: The adsorptive characteristics and elutive parameters of the process were studied by taking the elutive and purified ratio of total saponins of Rubus parvifolius L. as marker. Results: 19.3mL of the extractive of total saponins of Rubus parvifolius L. was purified with a column of macroreticular resin (R1cm?H20cm,dried weight 2.68g) and was washed with 100mL of distilled water, then was eluted with 100mL of 50% ethanol. Conclusion: With macroreticular resin to adsorb and purify, the elutive ratio of total saponins of Rubus parvifolius L. was over 79.2% and the purity reached 55.3%. So this process of applying macroreticular resin to adsorb and purify total saponins of Rubus parvifolius L. is feasible.

【Objective】 To investigate the prevalence of hepatitis E virus (HEV) among voluntary blood donors, and the viability and practicability of nucleic acid testing (NAT) in Jinhua area. 【Methods】 Blood samples from 1 017 voluntary blood donors at Jinhua Blood Center from April to May 2021 were collected, and HEV RNA was detected by CHURAS BSS1200 blood nucleic acid detection system. The reactive samples were re-tested for IgM and IgG antibodies by ELISA, and HEV genotyping by gene sequencing. 【Results】 The yield rate of HEV RNA out of voluntary blood donors in Jinhua was 0.98 ‰ (1 / 017). The individual was negative for IgM and IgG antibodies, but was confirmed to be HEV RNA genotype 4 by sequencing. 【Conclusion】 HEV RNA positive donors have been found in this study. HEV RNA blood screening can effectively enhance the safety of blood transfusion.

Objective To construct a framework and relevant evaluation items of the teaching ability for nursing teachers in colleges or universities.Methods The research was carried out by a variety of means such as theory analysis,literature study,expert consultation,etc,to explore behavior items that could be used to evaluate the teaching ability for nursing teachers in colleges or universities.A questionnaire was formed and a survey was conducted in a total of 194 nursing teachers from ten colleges or universities across the whole nation.Then,factor analysis was performed to build the relevant evaluation items for teaching ability of full-time nursing teacher.Cronbach's α and Spearman correlation were used to test the reliability and validity.Results The relevant evaluation items of teaching ability for nursing teachers contained 31 behavior items from six domains,which were teaching research ability,emotional teaching ability,expressing ability,self-examination ability,integration ability,and organizing ability.Cronbach's α of the total system was up to 0.913 0,Cronbach's α of each domain ranged from 0.681 0 to 0.815 8.Every factor had positive correlation with the totality,and the correlation coefficient of every item with its affiliated factor was greater than other factors.Conclusions The relevant evaluation questionnaire of teaching ability for nursing teachers has excellent reliability and validity.It can provide reference standard to directing admission,training,and teaching evaluation for nursing teachers.

【Objective】 To explore the intention of voluntary blood donation in students from a middle vocational school in Jinhua and its influencing factors. 【Methods】 A total of 400 students were enrolled from a middle vocational school in Jinhua by convenience sampling, and a self-designed questionnaire was issued to each student to investigate the cognition, intention and attitude of voluntary blood donation. The influencing factors of voluntary blood donation intention were analyzed by Logistic regression. 【Results】 A total of 394 valid questionnaires(98.50%) were collected. For 394 surveyed students, the average score of cognition of voluntary blood donation was (7.27±2.69) points, and the overall rate of intention to voluntary blood donation was 21.32%. Logistic regression analysis equation consisted of family support, better cognition, feeling happy about blood donation and worrying about blood donation, with risk ratios(OR) as 31.78, 188.69, 26.27 and 0.01, respectively(all P<0.05). 【Conclusion】 Students from a middle vocational school showed poor congnition and intention of voluntary blood donation.Family support for voluntary blood donation, better cognition of voluntary blood donation and happiness for blood donation were positive factors, and the fear of blood donation was a negative factor.It is necessary to strengthen the publicity of blood donation among students in middle vocational school to improve their congnition and intention to voluntary blood donation.

Objective To study the effects of miR-200b on proliferation and migration of sw620 colon cancer cells,and its regulation effect on E-cadherin expression.Methods The expressions of miR-200b in sw620 cells at 24 h and 72 h after pEGP-miR-200b transfection were detected by real-time PCR (RT-PCR).The change of the expression level of E-cadherin after miR-200b transfection was detected using the methods of RT-PCR and Western blot.The proliferation and migration abilities were measured by MTT and scratch test after miR-200b transfection.Results The expressions of miR-200b in sw620 cells at 24 h and 72 h after pEGP-miR-200b transfection raised significantly compared to the control group (t =11.579,P ＜ 0.01 ; t =11.579,P ＜0.01).MiR-200b transfection inhibited the proliferation abilities of sw620 cells.It is the most significant of the inhibitory effect on the third day and the inhibition rate was 55.34％.MiR-200b transfection markedly inhibited the migration abilities of sw620 cells.The two groups had significant difference in the migration distance of 24,48,72 h (t =11.579,P ＜0.01 ; t =10.419,P ＜0.01 ; t =6.955,P ＜0.01).The mRNA and protein expressions of E-cadherin gene increased significantly by transfecting miR-200b gene in sw620 cells (t =10.432,P ＜ 0.01 ; t =8.325,P ＜ 0.O1).Conclusion Up-regulated expression of miR-200b could inhibite the proliferation and migration abilities of sw620 colon cancer cells.The involved molecular mechanism is probably related to the change of E-cadherin expression.

A good funds budget can optimize efficiency of the research funds. Through the implementation of a National Science and Technology Major Project, the authors analysed the budget management proposed some skills and suggestions for the planning and management of budget.

Objective To study the expression of transcription factor Sp1 and CEA and the correlation between the two tran‐scription factors in colorectal cancer .Methods To detect expression Sp1 and CEA mRNA by Real‐Time PCR in 60 colon cancer tis‐sues and corresponding normal tissues and the results were compared with the clinical features and pathological characters .The re‐lationship between the expression of Sp1 mRNA and CEA mRNA in 60 colon cancer tissues was determined .Results The expres‐sion rates of Sp1 and CEA mRNA was detectable to highly expressed rates in colon cancer tissues than the matched normal tissues (P<0 .01) .There was no significant correlation between Sp1 and CEA mRNA expression in age ,sex ,tumor location(P>0 .05) . Sp1 and CEA mRNA was detectable to highly expressed in the different histological grade and Dukes stages .In addition ,a positive correlation was found between the expression of Sp1 mRNA and CEA mRNA(r=0 .706 ,P<0 .01) ,(0< r<1) .Conclusion Sp1 and CEA was detectable to highly expressed in colon cancer .Positively correlation occurred in Sp1 mRNA and CEA mRNA indica‐ted that Sp1 and CEA provide the new clues of genetic diagnosis and treatment .

ObjectiveTo investigate the association between catechol-O-methyl transferase (COMT)gene polymorphism and Gilles de la Tourette' s syndrome(GTS).MethodsUsing Amplification Refractory Mutation System(ARMS) PCR genotyping assay method,a polymorphism (val158met) of COMT gene was genotyped in 112 of all GTS patients ( total GTS group) including 54 GTS-alone patients group,48 GTS + ADHD patients group among of them and 71 healthy controls.The correlation between positive association of polymorphism (val158met)of COMT gene in GTS and the age of onset in patients with GTS was also analyzed.ResultsCompared with healthy controls group,genotype of val158met did not differ in total GTS patients group or alone-GTS patients group (χ2 =0.56,P=0.756;χ2 =1.05,P=0.600 respectively).There was also no significant difference (P＞0.05)in allele distribution of val158met in total GTS patients group or alone-GTS patients group compared with controls group respectively (χ2 =0.18,P=0.669;χ2 =0.29,P=0.593 respectively).However,genotype distribution of val158met was significantly different between GTS + ADHD patients group and controls group( χ2 =6.35,P =0.041 ).The frequency of the val allele of this locus was significantly higher in GTS + ADHD patients group than those in controls group ( χ2 =5.49,P =0.019 ).The mean age of onset (6.80 ± 1.54 ) in 36 children within GTS + ADHD patients group with the val/val geantype of COMT gene val158met polymorphism was significantly earlier than the mean age of onset (8.04 ± 1.54)in 12 children in val/val genotype (P =0.016 ).ConclusionPolymorphism (val158met) of COMT gene may be associated with GTS children with comorbid ADHD,which may play an important role to make the age of onset in children with GTS become earlier.