Cephalosporins are widely used antibiotics owing to their broad activity spectra and low toxicity. Many of these medically important compounds are made chemically from 7-aminodeacetoxycephalosporanic acid. At present, this intermediate is made by synthetic ring-expansion of the inexpensive penicillin G to form G-7-ADCA, followed by enzymatic removal of the side chain to obtain 7-ADCA. The chemical synthetic process is expensive, complicated and environmentally unfriendly. Environmentally compatible enzymatic process is favorable compared with chemical synthesis. In our previous research, metabolic engineered Escherichia coli strain (H7/PG15) was constructed and used as whole-cell biocatalyst for the production of G-7-ADC with penicillin G as substrate. The whole-cell biocatalysis was studied by single factor experiment, including the composition of substrates and the conversion conditions (OD600, pH, concentration of penicillin G, MOPS, glucose, time and FeSO4). After optimization, 15 mmol/L of G-7-ADCA was obtained. The process is convenient, efficient and economic. This work would facilitate the industrial manufacturing and further product research.
Anti-Bacterial Agents ; biosynthesis ; Biocatalysis ; Cephalosporins ; biosynthesis ; Escherichia coli ; metabolism ; Metabolic Engineering

s] Objective To analyze the diagnostic value of combined detection of sFIt-1, PLGF and Survivin in early onset preeclampsia. Methods From January 2017 to January 2018, 100 patients with early-onset PE were selected as observation group and 100 healthy pregnant women as control group in Tangshan Maternal and Child health Hospital Gynecology and Obstetrics. The expression levels of sFIt-1, PLGF and Survivin in serum were detected by enzyme-linked immunosorbent assay (ELISA), and the diagnostic value of each index was analyzed separately and jointly. Results The levels of sFIt-1 in the observation group were significantly higher than those in the control group: (36.58 ± 18.34) μg/L vs. (28.43 ± 3.28) μg/L (P<0.05), and the levels of PLGF and Survivin in the observation group were significantly lower than those in the control group: (213.18 ± 48.23) ng/L vs. (398.17 ± 41.19) ng/L, (0.72 ± 0.29) μg/L vs. (1.43 ± 0.32) μg/L (P<0.05); 103 cases of positive sFIt-1, 108 cases of positive PLGF, 107 cases of positive Survivin, 121 cases of positive parallel combined diagnosis and 121 cases of positive series combined diagnosis were found. The sensitivity and negative predictive value of parallel combined diagnosis were significantly higher than those of individualized diagnosis (P<0.05), and the specificity and positive predictive value of series combined diagnosis were significantly higher than those of individualized diagnosis (P < 0.05). Conclusions The combined detection of sFIt-1, PLGF and Survivin in serum can effectively improve the diagnostic accuracy of early-onset preeclampsia and has high clinical value.

Objective To explore the effects of long non-coding RNA(lncRNA)small nucleolar molecule RNA host gene 12(SNHG12)targeting inhibition of miR-495-3p/phospholipinositol-3-kinase(PI3K)/protein kinase B(Akt)signaling pathway on the proliferation,migration and invasion of prostate cancer cells.Methods The expressions of SNHG12 and miR-495-3p in prostate cancer tissues and cells(LNCaP,C4-2,DU145)were detected with real-time fluorescence quantitative PCR(qRT-PCR).After DU145 cells were divided into si-NC,si-SNHG12,si-SNHG12+anti-miR-NC and si-SNHG12+anti-miR-495-3p groups,the expressions of SNHG12 and miR-495-3p were detected with qRT-PCR;the targeting relationship between SNHG12 and miR-495-3p was determined with dual luciferase assay;cell proliferation was assessed with MTT assay;cell migration and invasion were evaluated with Transwell assay;the protein expressions of proliferating cell nuclear antigen(PCNA),N-cadherin,and E-cadherin were detected with Western blot.Results The expressions of SNHG12 were significantly increased,while the expression of miR-495-3P was significantly decreased in prostate cancer tissues and cells(LNCaP,C4-2,DU145)(P＜0.05).Knockdown of SNHG12 decreased DU145 cell activity,lowered the protein expressions of PCNA and N-cadherin,reduced the number of migrating and invading cells,but increased the protein expression of E-cadherin(P＜0.05).SNHG12 targeted and negatively regulated miR-495-3p,and down-regulation of miR-495-3p reversed the effects of SNHG12 knockdown on the proliferation,migration and invasion of prostate cancer cells.Compared with the si-NC group,the si-SNHG12 group had significantly decreased expressions of p-PI3K and p-Akt(P＜0.05).Compared with the si-SNHG12+anti-miR-NC group,the si-SNHG12+anti-miR-495-3p group had significantly increased protein expressions of p-PI3K and p-Akt(P＜0.05).Conclusion lncRNA SNHG12 can promote the proliferation,migration and invasion of prostate cancer cells through targeted inhibition of miR-495-3p/PI3K/Akt signaling pathway.