AIM To investigate the mechanism of anti-tumor effects of curcumin on human SPC-A1 cells. METHODS Cell culture、fluorescence microscope、TUNEL method and irradiation immunity techniques were used. RESULTS ①By the action of curcumin, SPC-A1 cells grew against the wall and then peeled off gradually;fluorescence microscope showed the nucleus broke into pieces, and cell split into apoptosis bodies in different sizes; The TUNEL method further certified that apoptotic cells reached 42 67% at the concentration of 20 ?mol?L -1 curcumin at 24 h. ②The level of intracellular cAMP concentration in the SPC-A1 cells,treated with curcumin, was significantly higher than that of the control group. ③Treated with 20 ?mol?L -1 curcumin for 24 h, the expression of Caspase-8 mRNA of human SPC-A1 cells was up-regulated. CONCLUSION Curcumin can induce the apoptosis of SPC-A1 cells, and one of its molecular mechanism may be associated with the rising of the level of intracellular cAMP concentration, and up-regulating expression of Caspase-8.

AIM: To study the protective effects of baicalin on myocardial ischemia-reperfusion injury in rats. METHODS: The models of myocardial ischemia-reperfusion injury were established by occluding left anterior descending coronary artery (LAD) for 30 min, followed by reperfusion for 120 min. The rate of rise and decline of left ventricular pressure (±dp/dtmax) and end-diastolic pressure of left ventricle (LVEDP) were monitored continuously with polygraph. After reperfusion, the blood and myocardium samples were taken for determination of malondialdehyde (MDA) content, superoxide dismutase (SOD), Na+-K+-ATPase, Ca2+-ATPase activities in myocardium, creatine kinase (CK) and lactate dehydrogenase (LDH) in serum with spectrophotometer. The ultrastructural changes in ischemic myocardium were assessed by transmission electron microscope. RESULTS:dtmax and LVEDP, decreased plasma CK and LDH levels, reduced myocardial MDA content, and increased the activities of SOD, Na+-K+-ATPase, and Ca2+-ATPase in myocardium following ischemia-reperfusion. The ultrastructural injury in reperfused myocardium was relieved. CONCLUSION: Baicalin possesses a protective effect against myocardial ischnemia-reperfusion injury through scavenging oxide radicals and improving Na+-K+-ATPase and Ca2+-ATPase activities.

Objective To explore the developmental toxicity of tributyltin chloride and the effects on sex hormone in female offspring rats through maternal gestation exposure. Methods Pregnant Wistar rats were randomly divided into four groups, 4 in each group. They were treated with TBTC by gavage at the dose of 0, 1, 2.5 and 5 mg/kg bw respectively from days 12-20 of gestation. 10 female offspring rats were randomly selected from each group and killed on postnatal day 70. The liver, kidney, uterus and ovary were weighed and the organ indexes were calculated. Pathological examination for liver, kidney, uterus and ovary were performed. Follicle stimulating hormone(FSH), luteotropic hormone(LH), testosterone(T) and estradiol(E2) in serum was determined by radioimmunity method. Results Increase of body weight in 2.5, 5 mg/kg bw groups significantly decreased(P

To investigate the effective principles of early management in extensively burned patients,162 patients (from 1990 to 1999) with extensive burn were treated according to the following principles: ① prompt and rapid fluid resuscitation during shock stage for delayed resuscitation patients; ② early tracheostomy,air way humidification and lavage for moderate and severe inhalation injury;③ escharectomy and grafting performed during early stage, presenting the experiences of escharectomy during shock stage in grassroot hospitals ; ④ undergoing enteral nutritional support in early stage; ⑤ emphasizing the importance of functional excise in early stage to stress that rehabilitation therapy should be implemented throughout that whole course of treatment. The results showed that the successful rate of treatment was raised and the incidence of complications and disability decreased after implementing the aforementioned treatment principles in extensively burned patients. It is suggested that the application of “Five early Principle” on extensively burned patients is effective and feasible

Objective:To study the effect of diammonium glycyrrhizinate (DG) on left ventricular function impairment during myocardial ischemia－reperfusion in rats.　Methods:The left ventricular was cannulated through right common carotid artery on the myocardial ischemia－reperfusion injury model. The left ventricler function was assessed before and 10 minutes after the administration of DG, immediate and 20 minutes after initiation of ischemia, immediate and 30, 60 minutes after reperfusion, by measuring the left ventricular systolic pressure (LVSP), left ventricular end diastolic pressure (LVEDP), ＋dp*dtmax and －dp*dtmax. 　Results:Compared with the baseline, all measurements of LVSP, ＋dp*dtmax and －dp*dtmax decreased (P＜0.05,P＜0.01), while LVEDP increased (P＜0.01) after administration of DG. As the time of ischemia－reperfusion lasting, LVSP, dp*dtmax and －dp*dtmax generally decreased progressively, while LVEDP generally increased progressively. 20 minutes after the initiation of ischemia and immediate, 30 and 60 minutes following reperfusion, LVSP, ＋dp*dtmax and －dp*dtmax in the DG group were significantly higher than those in IR group (P＜0.01), LVEDP significantly lower than that in IR group (P＜0.01) at the same time. 　Conclusion:DG can protect left ventricular function against myocardial ischemia－reperfusion injury in rats.

Objective To learn the policy outcomes of the management of category-B large medical instruments deployed in Beijing.Methods Call into play the data from the surveys made in 2005,2007 and 2010 on the five types of category-B large medical instruments deployed in the city.Summarize and analyze the outcomes of such instruments in terms of their total configuration volume,and the growth,distribution and use.Results From 2005 to 2010,CT scored the fastest growth of these five types of instruments,with 48 units deployed; SPECT was the lowest,with 8 units deployed.Conclusion The volume growth,instruments distribution,and use efficiency of such instruments in Beijing are rationally evolving,which proves that the deployment and management of category-B large medical instruments is compliant with conditions of the city.

Objective To investigate the relationship between disease activity and osteocalcin,β-isomerized C-terminal telopeptides (β-CTX) in SAPHO syndrome.Methods We studied 58 cases who were diagnosed as SAPHO syndrome.The following indices used to assess the activity of SAPHO were measured:Bath ankylosing spondylitis Disease activity index (BASDAI),Bath ankylosing spondylitis functional index(BASFI),visual analogue scale (VAS) pain and erythrocyte sedimentation rate (ESR),C-reactive protein (CRP),osteocalcin and β-CTX.The SAPHO patients were divided from two groups according to the disease activity.The relationships were analyzed with t-test and Pearson's test.Results The β-CTX of the active group(0.43±0.20) ng/ml was elevated than the stable group (0.23 ±0.09) ng/mL (t=-4.620,P=0.007).And there was no significant difference in the osteocalcin between the active group (2.7±1.0) μg/L and the stable group(2.4±1.0) μg/L (t=-1.169,P=0.820).There was no significant correlation between the serum osteocalcin and ESR or hypersensitive CRP (hs-CRP) (rESR=0.228,PESR=0.088;rhsCRP=-0.085,PhsCRP=0.528).And β-CTX level was related to the VAS (r=0.496,P＜0.01),BASDAI (r=0.401,P=0.002) and BASFI (r=0.295,P=0.025).While no significant relationship between the osteocalcin and pain index was observed in our study.Conclusion The positive correlation between serum β-CTX and disease activity indicates significant bone absorption during the bone destroy process in SAPHO syndrome.

Objective To investigate the sedative effects and the adverse reactions in the elderly patients received different speed of dexmedetomidine (Dex) intravenous infusion. Methods Eighty elderly cases were randomly divided into four groups. Group D0 was the control group, while the group D1, D2 and D3 were the trial groups. The heart rates, blood pressure, SpO2, Ramsay sedation score and Narcotrend value were recorded. Results The sedation onset time of the D2, D3 group was faster than those in the D0 and D1 groups (P <0.05, respectively), and the duration of sedation in groups D2 and D3 were significantly longer than that in the D0 and D1 groups (P < 0.05). Among the four groups, no significant differences in the incidence of hypotension or bradycardia needed vasopressors or atropine to treat and oxygen saturation were shown (P > 0.05). Conclusion Intravenous infusion of Dex by doses of 0.75 ~ 1.0 μg/(kg·h) during hip surgery in the elderly patients under spinal anesthesia could lead to a safe and effective sedation.