Objective To analyze the effects of vascularized fibular flap combined with vacuum sealing drainage (VSD) in the repair of tibia defect.Methods Clinical data of 24 patients with chronic osteomyelitis of the tibia who accepted the operation of vascularized fibular flap combined with VSD were collected in the study.In the first operation, the lesions were completely debrided and the dead bone was removed, then the vascularized fibular was replaced from the other limb to repair the tibia defect.Later, vacuum-assisted closure was applied to cover the wound.Results All patients enrolled in the study were followed up for 13 to 50 months, with an average of 32.3 months.The sinus of 22 cases healed in 4 weeks and the healing rate was 91.7%.The other two patients received additional debridement for the sinus and they also healed soon after that.All flaps survived and the survival rate was 100%.The X-ray showed that the transplanted fibula was healed in all cases, with a healing rate of 100%.The healing time ranged from 4 to 7 months, with an average of 4.9 months.Based on the Enneking scoring system, the patients achieved an average score of 26 points out of 30 points, and the recovery rate of limb function was evaluated as 86.7%.Conclusion For patients with chronic osteomyelitis of the tibia, vascularized fibular flap combined with VSD can not only effectively repair bone defect, but also restore the continuity of limbs.The application of VSD can effectively control infection, shorten treatment course, and restore limb function.

BACKGROUND:Primary vascular endothelial cels are mostly harvested through aorta endothelial cel cultures and micro-artery endothelial cel cultures using enzyme digestion and tissue adhesion methods, and the quality and purity of harvested cels cannot meet the need for current scientific research.
OBJECTIVE:To investigate an improved extraction of primary vascular endothelial cels and the relevant identification method.
METHODS: A segment of rabbit aorta was cut to culture vascular endothelial cels using the improved extraction method in group A or using adhesion method in group B. In the group A, the vascular intima was striped out with microsurgical instruments, and digested enzymaticaly to acquire single primary cels folowed by culture in endothelial cel culture medium. In the group B, the whole vascular intima was adhered to the culture dish that was incubated in a 5%CO2, 37℃ incubator for 1 hour. Cel pelets in the two groups were culturedin vitro. Cel morphology was observed using a microscope; immunohistochemical staining was used to detect CD31, VIII factor and Vimentin protein for identification of vascular endothelial cels.
RESULTS AND CONCLUSION:The purity and number of vascular endothelial cels extracted by the improved method were higher than those by the adhesion method. Immunohistochemical findings showed positive expression of CD31 and VIII factor, but negative expression of Vimentin. These findings indicate that the improved extraction method can obtain more vascular endothelial cels with higher purity, which is of strong operability and practicality.

Objective: To establish the determination method of ester alkaloids in Shiwuweirupeng Capsules. Methods:The chromatographic method was carried out on Aichrom TM C 18 column using CH 3OH 0.05 mol?L -1 KH 2PO 4 CH 3COOH-(CH 3) 2CHOH(67∶173∶4∶4) as a mobile phase. The detection wavelength was set at 230nm. The flow rate was 1mL?min -1 . The column temperature was 35?C. Results: The benzoic acid calibration curves were linear at the ranges of 0.0152～0.076?g( r =0.9998). The average recoveries of aconitine was 93.3% with RSD =1.9%.Conclusion: This method is simple, reliable and provides a reference standard for the quality control of Shiwuweirupeng Capsules.

Objective: To determine tannins in Ershiwuweidatang Capsules (Flos Carthami, Fructus Chebulae, Fructus Terminaliae Billericae, Fructus Phyllanthi, etc.). Methods:Tannins were determined by casein method.Results: The contents of tannins in Ershiwuweidatang Capsules were 0.96%, 0.90%, and 0.85%, respectively. The gallic acid calibration curves were linear at the ranges of 0.8 ～4.0 ?g?mL -1 ( r = 0.999 ). The average recovery of tannic acid was 96.2% with RSD =1.4%( n =5).Conclusion: The method was simple and reliable.

AIM:To prepare Wurenchun Dispersible Tablet(Fructus schisandrae Chinensis)and investigate the dissolution of it.METHODS:The optimal formulation of Wurenchun Dispersible Tablets was established by the single factor experiments.The dissolution of deoxyschisandrin from Wurenchun Dispersible Tablets was determined by HPLC.RESULTS:Wurenchun Dispersible Tablets prepared by the mixture of 50 g extraction and 125 g CaHPO_4 plus excipent(MCC∶CMS-Na 3∶1)could disintegrate and disperse well within 3 min in(20?1)℃ water.The dissolution of deoxyschisandrin from Wurenchun Dispersible Tablets was higher than 80% in 15 min.CONCLUSION:Wurenchun Dispersible Tablets were stable,dissolved fast and completely.The preparation could promote the dissolution of total lignans.

OBJECTIVETo establish a HPLC-PDA method for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang.

METHODA Symmetry Shield RP18 (4.6 mm x 250 mm, 5.0 microm) was used with a mobile phase of acetonitrile-0.01% H3PO4 in gradient elution. The detection wavelength was 251 nm,the flow rate was 0.45 mL x min(-1) and the column temperature was maintained at 30 degrees C.

RESULTThe accuracy, precision, sensitivity, specificity and linearity of this method met the requirements. The contents of the five effective fractions were determined simultaneously.

CONCLUSIONThe method is rapid,simple and accurate and it can be suitable for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang simultaneously.

Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; chemistry ; Flavanones ; analysis ; Flavonoids ; analysis ; Glucosides ; analysis ; Glycyrrhizic Acid ; analysis ; Indicators and Reagents ; analysis ; Medicine, Chinese Traditional ; Rehmannia ; chemistry ; Spectrophotometry, Ultraviolet ; methods ; Technology, Pharmaceutical

A HPLC method for determination of limonin, evodiamine and rutaecarpine in Evodia rutaecarpa was optimized. The mobile phase was [acetonitrile-tetrahydrofuran (25: 15)] -0.02% H3 PO4 (35:65). The detection wavelength was 220 nm and the flow rate was 1.0 mL x min(-1). Limonin, evodiamine and rutaecarpine were all well separated from other substances and their UV spectrums were essentially the same to the standards . The liner ranges of limonin, evodiamine and rutaecarpine were 0.196 8-3.936, 0.153 6-3.072, 0.097 4-1.948 microg. The average recoveries were 97.8%, 100.7% and 98.4%. RSD were 1.7%, 1.3% and 1.1% (n = 6). The method of this article is accurate, reproducible and can be used to enhance the quality control of E. rutaecarpa.
Chromatography, High Pressure Liquid ; methods ; Evodia ; chemistry ; Indole Alkaloids ; analysis ; Limonins ; analysis ; Plant Extracts ; analysis ; Quinazolines ; analysis

OBJECTIVETo establish the HPLC fingerprint and determine six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice.

METHODHPLC analysis was performed on a Phenomenex Gemini C18 (4.6 mm x 250 mm, 5 microm) with 0.04 mol x L(-1) ammonium acetate (adjusted to pH 10.0 with ammonia water)and acetonitrile as mobile phase. Similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine (2004 AB)was used in data analysis.

RESULTBaifupian, Heifupian from different samples were of high similarity in fingerprint, and the separation of six kinds of ester-type alkaloids was good. Huangfupian and crude aconite root showed significant difference in fingerprint, comparing with Baifupian and Heifupian.

CONCLUSIONThis method is simple and reliable. The HPLC fingerprint and contents of six kinds of ester-type alkaloids of Radix Aconiti lateralis praeparata slice can be used for their quality control.

Aconitum ; chemistry ; Alkaloids ; analysis ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; standards ; Medicine, Chinese Traditional ; standards ; Quality Control

OBJECTIVETo determine carboxyatractyloside and atractyloside in Xanthii Fructus by HPLC.

METHODBy HPLC, Agilent ZORBAX SB-phenyl (4.6 mm x 250 mm, 5 microm) column was adopted, with acetonitrile-0.01 mol x L(-1) NaH2PO4 (pH 6) as the mobile phase for gradient elution at the flow rate of 1.0 mL x min(-1). The detection wavelength was 203 nm, and the temperature was set at 35 degrees C.

RESULTCarboxyatractyloside showed a good linearity within the range of 0.0972-1.944 microg and atractyloside showed a good linearity within the range of 0.1030-2.060 microg. The recovery rate of carboxyatractyloside was 100. 3% and that of atractyloside was 102.5%. The RSD were 0.67% and 1.4% (n=6).

CONCLUSIONThis method is so simple, practical and highly repeatable that is can be used for quality control of Xanthii Fructus.

Asteraceae ; chemistry ; Atractyloside ; analogs & derivatives ; analysis ; isolation & purification ; Chromatography, High Pressure Liquid ; methods ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Fruit ; chemistry

Objective To investigate the efficacy of suprapatellar versus infrapatellar approach in tibial intramedullary nail fixation for tibial fracture. Methods Clinical trials that evaluated suprapatellar approach and infrapatellar approach for tibia intramedullary nailing fixation were searched in PubMed, EMBASE, Cochrane Central Register of Controlled Trials ( CENTRAL ) , Chinese Biology Medicine ( CBM) , Wanfang, Weipu and CNKI databases. Methodological qualities of the included studies were assessed using the Cochrane Collaboration tool and Newcastle-Ottawa Scale. Sensitivity analyses were performed to determine whether overall results were reliable. Publication bias was detected using Begg's test and Egger's test. Lysholm score, reoperation rate, Hospital for Special Surgery ( HSS ) score, operation time, intraoperative blood loss and hospital stay were selected to evaluate the clinical effect of suprapatellar approach and infrapatellar approach in tibial intramedullary nail fixation for tibial fracture. Results Two randomized controlled trials and six case-controlled studies involving 787 patients were included in the analysis. Sensitivity analyses indicated that the results were statistically significant. No significant publication bias was detected by Begg's test or Egger's test. Our meta-analysis indicated that suprapatellar approach had significantly higher Lysholm score ( MD=1. 04, 95％CI 0. 82 -1. 26, P<0. 05, I2 =10. 5％) and HSS score ( MD =0. 97, 95％CI 0. 65 -1. 30, P <0. 05, I2 =0 ) than infrapatellar approach ( P <0. 05 ) . Additionally, there were no statistical differences between the two procedures in reoperation rate, operation time, intraoperative blood loss and hospital stay (P>0. 05). Conclusion Compared with infrapatellar approach, suprapatellar approach in intramedullary nail fixation for tibial fracture can better relieve the pain, keep stability and motion of the knee joint and promote function recovery of knee joint.