Setting criterion of teaching system,improving examination methods and strengthening the cultivation of basic medical skills and practice ability might be beneficial to the increase of the practice quality.

Objective To observe the changes of cerebral CT and auditory event related potentials (ERP, P300) for finding the effective method for the comprehensive evaluation of the patients with Alzheimer's disease. Methods Cerebral CT, P300, and Mini Mental State Examination (MMSE) were employed to assess 30 patients with Alzheimer's disease. Results Latency and amplitude of wave of P300 in patients were prolonged and decreased, respectively. Cerebral CT found an obvious atrophy in the frontal and temporal lobes of cerebra. These changes were obviously correlated with the severity of dementia. Conclusion Using cerebral CT, P300, and MMSE can offer morphometric, functional and psychological information which is helpful for precise assessment of patients with Alzheimer's disease.

ObjectiveTo understand the epidemiological characteristics of influenza in Jingzhou from 2016 to 2021， so as to provide scientific evidence for the formulation of influenza prevention and control policies in this region， and effectively protect people's health. MethodsData of influenza-like illness （ILI） and pathogen surveillance in Jingzhou during 2016‒2021 were collected and statistically analyzed. ResultsA total of 46 272 ILI cases were reported from two hospitals in Jingzhou City from 2016 to 2021. The difference in the constituent ratio of ILI was statistically significant among different age groups （P<0.05）. A total of 12 812 specimens were collected from two hospitals for influenza surveillance. A total of 1 513 cases were RNA positive，and the positive rate of influenza virus nucleic acid detection was 11.81％. The RNA positive specimens were mainly B （Victoria）， accounting for 39.33％. There were statistically significant differences in the positive rate of influenza virus nucleic acids and different types of influenza virus nucleic acids among different years （P<0.05）. ConclusionThe influenza epidemic in Jingzhou peaks in winter and spring， and the new A （H1），A （H3）， B （Victoria） and B （Yamagata） types alternate and mixed epidemics dominate.

Objective:To investigate the effect and mechanism of Fuzheng Jiedu Tongluofang on the expression of MMP-2,MMP-9 and invasion of human hepatoma HepG2 cell.Methods:Hepatioma HepG2 cell was treated with Fuzheng Jiedu Tongluofang.The cell viability was measured by CCK8.The invasion of HepG2 was detected by Transwell assay.The expression of MMP-2 and MMP-9 was analyzed by ELISA.Results:In the CCK8 assay,Fuzheng Jiedu Tongluofang could inhibit the proliferation of HepG2 cells at a density and time measure.In the Transwell assay,the inhibitory rate of invasion was 52.45% when treated with Fuzheng Jiedu Tongluofang at a concentration of 2.65 mg/ml. The ELISA assay indicated that the expression of MMP-2 and MMP-9 decreased significantly after treated with Fuzheng Jiedu Tongluofang(P<0.05). Conclusion:Fuzheng Jiedu Tongluofang suppressed the invasion of HepG2 with the possible mechanism of down-regulating the expression of MMP-2 and MMP-9 in HepG2.

Objective:To study the differential expression of VEGF, SDF-1 and CXCR4 in colorectal cancer tissue and paracancerous tissues, and analyze the correlation between the three and the clinic pathological parameters of gastric cancer;to investigate the significance of the expression of VEGF,SDF-1 and CXCR4 in the progression of colorectal cancer.Methods:Immuno-histochemistry streptavidin-biotin complex( SABC) method and real-time Q-PCR were used to detect the operation resection of colorectal carcinoma and tumor-adjacent specimens of SDF-1,CXCR4 and VEGF expression.Results: On the mRNA level,compared with the adjacent tissues,the expression of SDF-1, CXCR4 and VEGF in colorectal cancer tissues was significant higher .The IHC′s results indicate that the positive rate of SDF-1,CXCR4 and VEGF in colorectal cancer were 78.7%,60% and 66.3%,the positive rate of adjacent tissues was 37.5%,42.5% and 28.8%, and there was statistically significant between expression and carcinoma adjacent tissue differences( P<0.05).There was statistically significant difference in the expression of SDF-1 with tumor metastasis,lymph node metastasis,TNM staging(P<0.05-P<0.01),while had no difference with gender,size and depth of invasion.There was statistically sig-nificant difference in the expression of CXCR4 with node metastasis,TNM in tumor staging ( P<0.05-P<0.01).It showed a significant negative correlation in SDF-1 expression and CXCR4,VEGF expression(P<0.01).Conclusion: It has a certain relationship between biological characteristics and SDF-1, CXCR4 and VEGF expression in colorectal cancer tissues.These results indicate that the expression of SDF-1,CXCR4 and VEGF play an important role in the tumorigenesis,progression,invasion and metastasis of colorectal cancer.

Embolization, Therapeutic ; Endoscopy ; Hematoma, Subdural, Chronic ; surgery ; Humans ; Meningeal Arteries ; Oxygen ; therapeutic use

AIM: To investigate the clinical effect and complication of orthokeratology for myopia.

METHODS: From August 2014 to October 2015, 204 adolescents(408 eyes)with myopia in our hospital, were treated with orthokeratology, and followed up at 1, 3, 6 and 12mo for visual acuity, corneal curvature, axial length and so on.

RESULTS: The visual acuity was significantly improved than before wearing(P<0.05). At 1, 3, 6 and 12mo after wearing, the visual acuity were 0.52±0.12, 0.64±0.20, 0.95±0.15 and 0.97±0.12; after wearing the diopter significantly decreased than before wearing(P<0.05); at 1, 3, 6 and 12mo after wearing diopter were 1.82±0.40D, 0.96±0.20D, 0.40±0.09D and 0.25±0.06D. There was no significant difference in the corneal curvature, axial length, corneal endothelial cell density and central thickness between before and after wearing(P>0.05). After wearing the anterior corneal surface curvature radius significantly increased than before wearing(P<0.05). At 1, 3, 6 and 12mo after wearing, anterior corneal surface curvature radius were 7.90±0.21 mm, 8.20±0.22mm, 8.24±0.23mm and 8.30±0.26mm. There was no significant difference on the posterior corneal surface curvature radius and anterior chamber depth between before and after wearing(P>0.05).

CONCLUSION: Orthokeratology correcting myopia, is safe and effective with no obvious effect on corneal endothelial cells and anterior chamber depth. There is a certain scope of application for the correction of myopia.

Objective Using the information - motivation - behavior model (IMB) to intervene with the alcohol dependence patients and provide a new strategy for implementing health education. Methods Eighty-nine patients with alcohol dependence, who clinically recovered or improved after the discharge, were randomly divided into a research group and a control group. Both groups received routine health education, rehabilitation guidance and a follow-up call. The research group implemented additional IMB health education. The implementation effect on alcohol dependence scale (ADS), Michigan alcoholism screening test (MAST), and alcohol dependence prevention and treatment knowledge (self-designed questionnaire) of the two groups before and after intervention was analyzed. Results The relapse rate of the research group was lower than that the control group, which was statistically significant (P＜0.05). MAST scale and ADS scale in the research group were lower than those in the control group within 3 months and 6 months after the discharge, which was significant (P values of both scales ＜0.05). After the 6-month follow-up, the knowledge of alcohol dependence patients in the research group and their families was significantly higher than that in the control group (P＜0.05). Conclusion IMB health education implementation for alcohol dependence patients significantly reduced the relapse rate, decreased physical and psychological dependence, and improved the ability of patients to prevent and control alcohol dependence.

Child ; Humans ; Facies ; Hyperventilation/genetics* ; Intellectual Disability/genetics* ; Mutation ; Transcription Factor 4/genetics*

In this paper, we analyzed medical records of 40 patients with coronavirus disease 2019(COVID-19), in order to explore the clinical efficacy of Matrine and Sodium Chloride Injection in the treatment of COVID-19. The investigation was based on the results of a previous animal test, which was aimed to investigate and confirme the clinical efficacy of Matrine and Sodium Chloride Injection in the treatment of COVID-19. The animal test demonstrated that Matrine and Sodium Chloride Injection has a significant therapeutic effect on the human coronavirus pneumonia for the model mice. The lung inhibition index reached up to 86.86%. The evaluation was conducted on 40 confirmed cases of COVID-19 treated at Jingzhou Hospital of Infectious Disease(Chest Hospital) of Hubei Pro-vince from January 30~(th) to March 21~(th), 2020. In these cases, patients were treated with other integrated Chinese and Western medicines regimens in the recommended Matrine and Sodium Chloride Injection diagnosis and treatment regimen. The clinical manifestations, laboratory data, nucleic acid clearance time, and imaging data were compared and analyzed before and after treatment. After administration with Matrine and Sodium Chloride Injection, the clinical symptoms of 40 cases were alleviated markedly, and their blood analysis and biochemical indexes returned to normal. The lung CT showed more than 50% of lesion absorption rate, and the viral nucleic acid test showed the average clearance time of patients was 16.6 days, and the average length of hospital stay was 25.9 days. After administration with Matrine and Sodium Chloride Injection, the symptoms of cough and fatigue were alleviated significantly, and the appetite was significantly improved compared with before, especially for patients with gastrointestinal symptoms. Additionally, laboratory indicators, especially absolute value and ratio of lymphocytes and CRP were significantly alleviated. According to the chest CT for short-term review, the absorption of lung lesions was faster than before, especially for grid-like and fibrotic lesions. Compared with antiviral drugs, such as Abidol and Kriging, the nucleic acid clearance time was significantly shorter than the cases treated with Matrine and Sodium Chloride Injection. The clinical effective rate of 40 cases was 100.0%. We believed that Matrine and Sodium Chloride Injection have a good clinical effect in the treatment of COVID-19, and suggested increasing the clinical application and further conducting large-sample-size cli-nical verification.
Alkaloids ; Animals ; Betacoronavirus ; Coronavirus Infections ; Disease Models, Animal ; Mice ; Pandemics ; Pneumonia, Viral ; Quinolizines ; Sodium Chloride ; Treatment Outcome