AIM: To observe the effects of IBMX on cGMP production in gerbil hippocampus after recirculation following ischemia. METHODS: Bilateral occlusion of common carotid arteries and immunofluorescent staining methods in gerbil hippocampal tissue slice were used. RESULTS: Recirculation following ischemia caused a rise in hippocampus cGMP concentration. IBMX increased cGMP production. cGMP positive cells mainly distributed in rediatum layer and molecular layer in the CA_1 subfield. CONCLUSION: IBMX increased cGMP production in gerbil hippocampus after recirculation following ischemia.

Objective To investigate the clinical effect of mesocaval shunt and selective coronary vein ligation for portal hypertension caused by cavernous transformation of the portal vein in adults.Methods The clinical data of 26 adult patients with portal hypertensive variceal bleeding caused by cavernous transformation of the portal vein treated by mesocaval shunt plus splenic artery and coronary vein ligation were analyzed retrospectively from 2001 to 2011.Results There was no operative deaths,free portal vein pressure decreased postoperatively,differences were significant before and after shunt (34.4 ± 4.7) cm H2O vs.(24.8 ±2.7) cm H2O,t =12.30,P ＜0.01.Postoperative complications included slight hepatic encephalopathy in 1 case,stress ulcer bleeding in 1 case,which were cured by conservative treatment.23 cases were followed up from 6 months to 4.5 years,and there was no bleeding case.Conclusious Mesocaval shunt plus selective vascular ligation for portal hypertensive variceal bleeding caused by cavernous transformation of the portal vein in adults is safe and effective.

The segment length,the length of dorsal root attachment of each segment of the spinal cord of 10 adult men were measured.The cross sections from 4 specimens were stained with 1% osmic acid and the area of each section was measured with a com- pensating polar planimeter after being magnified 10 diameters.The segment volume was obtained by multiplying the segment length by the cross-sectlon area. The results shows that the length of spinal cords in Chinese are essentially the same as those from European and Japanese.They are approximately 44cm.in length. The length of each part of the cord in Chinese,European and Japanese were compared. The length of each part of the cord in Chinese and European are essentially alike,but the length of lumbar cord of Japanese appears to be slightly longer. The results show that the weight of the segment was closely related to the segment length. The coronal diameter of each segment is longer than the sagittal diameter,especial- ly it is true in the cervical enlargement.The longest coronal and sagittal diameters are found in the cervical enlargement,the next are in the lumbar enlargement,and the shortest are in the thoracic segment. The volume of the cervical segment and the lumbo-sacral segment is closely rela- ted to their cross-section area particularly to the area of their white matter. The volume of the gray matter of the cervical and the lumbo-sacral segments has a close relation to the gray matter area of the same segment,but that of the thoracic segment is related to its length. It is quite obvious that the volume of the white matter of both the cervical and lumbar enlargements reaches its highest value at segments higher than that of the gray matter,which may indicate that the increase in volume in the gray and white matter are dependent upon each other.

Femurs of 206 Chinese adults (122 males and 84 females) were used for the present study. Their lengths, and torsional and inclinational angles were measured after Martin's method. The results are as follows:1. The Lengths of FemurThe average length of the femur in males is 43.68?2.02 cm. (43.77?2.02 cm. on the left side, and 43.61?2.04 cm. on the right), while that in females is 40.96?2.84 cm. (41.05?2.84 cm. on the left side and 40.93?2.84 cm. on the right). No significant difference has been found between the femoral lengths of both sides; however, there is a significant difference between both sexes.2. The inclinational angles of femoral neckTheir average value in the male is 128.46??6.58?(128.87??6.76?on the left side, and 127.46??6.60? on the right), but that in the female is 130.52??6.44?(130.88??6.10? on the left side, and 130.17??6.80? on the right). No significant difference exists between both sides; however, there is a significant difference between both sexes.3. The Torsional Angles of FemurTheir average value in the male is 11.95??8.89? (14.51??8.08? on the left side, and 9.20??7.75? on the right), whereas that in the female is 12.80??9.07? (15.95??9.63? on the left side, and 9.63??7.31? on the right). There is a significant difference between two sides, but no significant difference between both sexes.4. The relationship between the inclinational and torsional angles on one hand and the changes in the femoral lengths on the other has been studied by dividing the femurs into groups according to their lengths. It has been shown that the changes in the femoral length exert no effect upon the inclinational and torsional angles of the femoral necks, neither in the male nor in the female.Besides, a comparison has also been made between the femurs of both sides and both sexes, between the femoral lengths and the inclinational and torsional angles, and between the variant ranges of the torsional angles of femurs. A discussion on these problems has been made with reference to the racial differences.

Pathological pain or clinical pain is caused by tissue and nerve injuries, and is usually chronic and mainly divided into inflammatory pain and neuropathic pain. Pathological pain is typically characterized by hyperalgesia (increased responsiveness to noxious stimuli) and allodynia (painful responses to normally innocuous stimuli). The mitogen-activated proteins kinases (MAPKs) are a family of evolutionally conserved molecules that play a critical role in cell signaling, consisting of extracellular signal regulated kinase (ERK), p38, and c-Jun N-terminal kinase (JNK), which play an important role in neural plasticity of pathological pain. Inhibition of MAPKs alleviates inflammatory pain and neuropathic pain in different animal models. It is very important to study the inhibition of MAPKs as a therapeutic approach to treat pathological pain.

Objective To investigate the value of obli que coronal MR images in visualization of the anterior cruciafe ligment(ACL).Methods Conventional axial,oblique sagittal and coronal MR images were performed in 30 normal knees.Then oblique coronal T 2-weighted i mages,which was parallel to ACL,based on oblique sagittal T 2 -weighted images ,were acquired.The visualization of ACL on oblique coronal,oblique sagital and coronal T 2-weighted images was analysed.Results The visualization of the whole ACL was 100%(30/30 ) on one image of the oblique coronal T 2-weighted images, 83.3%(25/30) on on e image of the oblique sagaital T 2-weighted image. On the coronal T 2-weighte d images,the ACL appeared as segmental bundle.The whole ACL could not be visua lized on one image of coronal T 2-weighted images.Conclusion The whole ACL can be showed on one image of ob lique coronal T 2-weighted image,which is helpful in evaluation of the injure o f ACL.

Lesion was made individually to the various cortical areas in 14 rats.The cortico- fugal fibers terminating into cochlear nuclei were traced in sections by means of the modified Nauta and Fink-Heimer silver staining method.The results were as follows: 1.After damaging the auditory area or somato-sensory area 1(SI)of the cerebral cortex unilaterally,degenerating fibers were found bilaterally in the ventral and dorsal cochlear nuclei.This result showed that the above cortical areas have direct descending connection with the cochlear nuclei. 2.No degenerating fibers were found in the cochlear nuclei after the ablation of the motor or visual area of the cerebral cortex,which demonstrated that the cochlear nuclei do not receive descending fibers from the above cortical areas. 3.The pathway from the cerebral cortex to the cochlear nuclei was:The descending fibers which originated from the auditory area or SI area ipsilaterally passed through the capsula interna and basis pedunculi,and part of these fibers passed dorsally and downward to the lateral portion of the reticular formation of the midbrain;and from there the fibers passed through the lemniscus lateralis and dorsal acustic stria to the cochlear nuclei of the ipsilateral side.The cochlear nuclei in contralateral side also received the corticofugal fibers by way of the dorsal acustic stria.These fibers might cross to the contralateral side at the region in the posterior commissure,superior colliculus commissure and inferior colliculus commissure.

Objective To explore the risk factors on impact of early dislocation after hip arthroplasty,and provide patients with individ-ualized treatment programs. Methods We retrospectively analyzed the clinical data of displaced femoral neck fractures in 247 patients who accepted hip arthroplasty from January 2006 to December 2011,and a total of 8 potential factors associated with occurrence of early dislocation were evaluated by univariate analysis and multivariate logistic regression analysis. Results The univariate analysis showed that sex,surgical approach and femoral head size were correlated with early dislocation (P<0. 05). Multivariate logistic regression analysis showed that female (OR=1. 862),posterolateral surgical approach (OR=3. 478),the use of 22 mm femoral heads (OR=2. 558)were independent risk factors (P<0. 05). And use of≥30 mm femoral heads(OR=0. 635) was a protective factor(P<0. 05). Conclusion Occurrence of early dislo-cation after hip arthroplasty is mainly correlated with many factors,various risk factors should be taken into account in preoperative and intrao-perative and choose the optimal individualized treatment plan.

Objective To observe and assess the clinicopathological significance of smooth muscle differentiation in breast cancer stromal fibroblasts. Methods A monoclonal antibody recognizing ? smooth muscle actin was used to determine the smooth muscle differentiation of fibroblasts in 69 breast cancer(BC) tissues, compared with 8 breast tissues which were removed more than 5cm from the cancer margin comfirmed without cancer cells. The relationship between the smooth muscle differentiation and clinicopathological parameters including age, tumor size, lymph node metastatic status, histological grade and ER receptor status in invasive BC were analyzed. Results Stromal fibroblasts smooth muscle differentiation was defined in 55.0% of the invasive breast cancer tissues,whereas no immunostain was demonstrated in histological normal samples. Twenty eight of the 41 cases(68.3%) with matastatic lymph nodes showed stromal fibroblasts smooth muscle differentiation,which more than that of without lymphatic metastasis group (26.3%,P

Objective To test whether multiplex ligation-dependent probe amplification(MLPA)could be used for the prenatal detection of the most common aneuploidies of chromosomes 13,18,21,X,and Y.Methods 34 cases including 22 blood samples(12 with trisomy 21,1 with monosomy X,one male witll extra Y and 8 healthy persons),4 cord blood samples with Down syndrome and 8 amniotic fluid samples ( 1 with trisomy 21 and 7 normal fetuses)were recruited into this study.All samples were confirmed by karvotype analysis. DNA was extracted from blood and amniotic lysate was incubated with proteinase K.MLPA was used to determine the relative copy numbers.Results The resuhs were available within 48 h and were concordant with karyotype analysis in all but one case of amniotic fluid that was suggested to be triploid sample 69,XXY by MLPA or contaminated by maternal blood.This sample actually was found containing a number of red blood cells after centfifugation in test. In total,the concordance rate with clinical characteristics was 97.1%.The Ratio values of 13,18,21,X in normal samples were approaching 1.0 except chromosome Y having slightly higher variation in relative copy number.The difference of ratio means between the normal and trisomy 21 samples was statistically significant by one-way ANOVA(F=298.906.P=0.000).Conclusion Computer assisted MLPA with high sensitivity is a rapid,simple,automatic and reliable method for detection of common chromosomal aneuploidies.