Objective To discuss the diagnosis characteristics and clinical value of posterior vitreous detachment (PVD) by color Doppler flow imaging(CDFI). Methods One thousand and two cases were examed by CDFI for the diagnosis standard and property of PVD. Results Typical acoustic properties of PVD: the B scan may show a very fluid thick line. There were two types complete PVD and part PVD based on the vitreous separated from the optic nerve. Kinetic echography typically showed a very fluid, undulating after movement of the completely PVD. Part PVD had more than one dot with attachments to the retina and less mobile than complete PVD. There was not blood flow signal on the PVD by CDFI. Conclusions Ultrasonography for PVD not only exactitudes,but also provides foundation for clinic.

OBJECTIVE: To evaluate the effect and causes of failure of vitreoretinal(VR) surgery in rbegmatogenous retinal detachments associated with choroidal detachment. METHOD: Reviewing the operative effects of the vitreoretinal surgeries in 61 patients(61 eyes) with rhegmatogenous retinal detachment associated with choroidal detachment and PVR in this hospital. Vitrectomy, peeling of preretinal membranes, fluid/air echange and inert gas, silicone oil tamponade were used in these patients according to need. RESULTS: On discharge from the hospital, the postoperative effect obtained in 40 case(65.57%), and out of 35 eyes receiving the inert gas tamponade 26(74.3%)got ef fective pesults. Fourteen cases were followed up for 3 months(averge 9.5 months)and 10(7.4%)of them revealed stable. The factors of influencing VR surgery seemed to be the range of the choroidal detachments, number of opreative times, the inert gas tamponede and the time of corticosteroid application. The causes of failure of opreation might relate to severe and anterior PVR, and giant tears. CONCLUSIONS: The VR surgery was thought to be profitable in treating rhegmatogenous retinal detachment associated with choroidal detachment and PVR.

Choledochojejunostomy is a common surgical method for the treatment of organ diseases such as hepatobiliary, gastrointestinal and pancreatic diseases, but there are many complications related to the operation. Benign biliary-enteric anastomosis stricture (BBES) is the most common long-term complication after operation, which seriously affects the quality of life of patients. At present, bile duct reconstruction and endoscopic treatment are the main methods to deal with BBES, but there are some limitations. The new interventional therapy has the advantages of small trauma, good repeatability, high success rate and few complications. It has been applied in many medical centers. The main treatment methods include percutaneous transhepatic biliary balloon dilatation, percutaneous temporary stent placement, percutaneous transhepatic choledochoscopy, magnetic compression anastomosis and intrabiliary ablation. Up to now, there is no uniform standard for the choice of interventional therapy for BBES at home and abroad, so it is of great clinical significance to establish a standardized interventional therapy strategy.

Reduning nasal sprays were prepared from Reduning injection to explore the pharmacokinetics of its nasal administration in rabbits. Healthy rabbits were divided into three groups: blank group, nasal administration group, and intravenous injection group. Plasma samples were pretreated using acetonitrite to precipitate proteins. High-performance liquid chromatography(HPLC)was performed for the detection of chlorogenic acid. The results showed that chlorogenic acid was detected in the plasma samples of nasal administration group with the highest concentration occurring in 2-5 min after administration, which indicated that Reduning nasal sprays could be successfully absorbed from nasal mucosa and could rapidly metabolize in blood. Compared with intravenous injection group, nasal administration group had lower concentration of active ingredients, revealing that the transdermal absorption rate of Reduning nasal sprays requires further improvement.

OBJECTIVE: To investigate the regulatory role of long non-coding RNA Kcnq1ot1 in osteoclast differentiation, osteogenic differentiation and osteoporosis. METHODS: The expression of lnc-Kcnq1ot1, Bglap, Runx2, Alp, Bsp, Nfatc1, Mmp9, Ctsk and Oscar were detected by real-time quantitative PCR (qRT-PCR) in the femoral bones from mouse models of postmenopausal osteoporosis (ovariectomized mice, RESULTS: The expression of lnc-Kcnq1ot1 was significantly upregulated during osteoblast differentiation but downregulated in the bone tissues of osteoporotic mice and during osteoclast differentiation ( CONCLUSIONS Our data demonstrate that lnc-Kcnq1ot1 promotes osteogenic differentiation and alleviates osteoclast differentiation, suggesting the potential of lnc-Kcnq1ot1 as a therapeutic target against osteoporosis.
Animals ; Cell Differentiation ; Cells, Cultured ; Mice ; Osteoblasts ; Osteoclasts ; Osteogenesis