1.Determination of Anionic Surfactants in Waters by On-line Double Microporous Membrane Extraction-Flow Injection Spectrophotometry
Ping ZHAO ; Jinhui CHEN ; Jingze XIAO
Chinese Journal of Analytical Chemistry 2009;37(12):1853-1856
Anionic surfactants in waters were determined using on-line double microporous membrane extraction-flow injection analysis method. This method was based on the extraction of the ion-association complex (λ_(max)=650 nm) which formed from methylene blue cation and anionic surfactants. The on-line double microporous membrane liquid-liquid extraction phase separators were adopted. Optimization of variables of the flow injection manifold, groove mechanical dimension of the phase separator, and pore size of the membrane was performed. The proposed method had a linear range of 25.0-1000.0 μg/L(r≥0.999). The detection limit was 4.28 μg/L. The relative standard deviation(n=7) of different concentration was 0.7%-6.0%. The recovery was between 96%-110% and the sample throughput was 18 h-1. The method was applied to the analysis of standard Reference materials with satisfactory results. For practical application, two water samples were analyzed with the established method.
2.Alterations of the Ca²⁺ signaling pathway in pancreatic beta-cells isolated from db/db mice.
Kuo LIANG ; Wen DU ; Jingze LU ; Fei LI ; Lu YANG ; Yanhong XUE ; Bertil HILLE ; Liangyi CHEN
Protein & Cell 2014;5(10):783-794
Upon glucose elevation, pancreatic beta-cells secrete insulin in a Ca(2+)-dependent manner. In diabetic animal models, different aspects of the calcium signaling pathway in beta-cells are altered, but there is no consensus regarding their relative contributions to the development of beta-cell dysfunction. In this study, we compared the increase in cytosolic Ca(2+) ([Ca(2+)]i) via Ca(2+) influx, Ca(2+) mobilization from endoplasmic reticulum (ER) calcium stores, and the removal of Ca(2+) via multiple mechanisms in beta-cells from both diabetic db/db mice and non-diabetic C57BL/6J mice. We refined our previous quantitative model to describe the slow [Ca(2+)]i recovery after depolarization in beta-cells from db/db mice. According to the model, the activity levels of the two subtypes of the sarco-endoplasmic reticulum Ca(2+)-ATPase (SERCA) pump, SERCA2 and SERCA3, were severely down-regulated in diabetic cells to 65% and 0% of the levels in normal cells. This down-regulation may lead to a reduction in the Ca(2+) concentration in the ER, a compensatory up-regulation of the plasma membrane Na(+)/Ca(2+) exchanger (NCX) and a reduction in depolarization-evoked Ca(2+) influx. As a result, the patterns of glucose-stimulated calcium oscillations were significantly different in db/db diabetic beta-cells compared with normal cells. Overall, quantifying the changes in the calcium signaling pathway in db/db diabetic beta-cells will aid in the development of a disease model that could provide insight into the adaptive transformations of beta-cell function during diabetes development.
Animals
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Calcium
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metabolism
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Calcium Signaling
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drug effects
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Cell Membrane Permeability
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drug effects
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Cells, Cultured
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Down-Regulation
;
drug effects
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Endoplasmic Reticulum
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metabolism
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Glucose
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pharmacology
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Insulin-Secreting Cells
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cytology
;
drug effects
;
metabolism
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Mice
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Mice, Inbred C57BL
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Mice, Obese
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Potassium Chloride
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pharmacology
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Sarcoplasmic Reticulum Calcium-Transporting ATPases
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metabolism
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Sodium-Calcium Exchanger
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metabolism
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Thapsigargin
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pharmacology
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Up-Regulation
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drug effects
3.Bulk-like endocytosis plays an important role in the recycling of insulin granules in pancreatic beta cells.
Du WEN ; Yanhong XUE ; Kuo LIANG ; Tianyi YUAN ; Jingze LU ; Wei ZHAO ; Tao XU ; Liangyi CHEN
Protein & Cell 2012;3(8):618-626
Although bulk endocytosis has been found in a number of neuronal and endocrine cells, the molecular mechanism and physiological function of bulk endocytosis remain elusive. In pancreatic beta cells, we have observed bulk-like endocytosis evoked both by flash photolysis and trains of depolarization. Bulk-like endocytosis is a clathrin-independent process that is facilitated by enhanced extracellular Ca(2+) entry and suppressed by the inhibition of dynamin function. Moreover, defects in bulk-like endocytosis are accompanied by hyperinsulinemia in primary beta cells dissociated from diabetic KKAy mice, which suggests that bulk-like endocytosis plays an important role in maintaining the exo-endocytosis balance and beta cell secretory capability.
Animals
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Calcium
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metabolism
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Cytoplasmic Granules
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metabolism
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Diabetes Mellitus
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metabolism
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pathology
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Disease Models, Animal
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Dynamins
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metabolism
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Electric Capacitance
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Endocytosis
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physiology
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Insulin
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metabolism
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Insulin-Secreting Cells
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metabolism
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pathology
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Male
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Mice
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Mice, Inbred C57BL
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Patch-Clamp Techniques
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Photolysis
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Primary Cell Culture
4.Morphological Characteristics of Normal and Gynandromorphic Hyalomma asiaticum Schulze and Schlottke, 1930.
Ze CHEN ; You Quan LI ; Qiao Yun REN ; Jin LUO ; Yonghong HU ; Kai LI ; Guang Yuan LIU ; Jian Xun LUO ; Jingze LIU ; Hong YIN
The Korean Journal of Parasitology 2015;53(3):361-364
Gynandromorphic ticks are extremely rare, and often attract parasitologists' attention. During our examination of tick specimens, an engorged gynandromorph of Hyalomma asiaticum was noticed. This is the first record of gynandromorphic ticks from China. In this study, several important morphological structures of normal and gynandromorphic H. asiaticum were analyzed. Comparing to the normal H. asiaticum, the gynandromorphic specimen was a typical bipartite protogynander. Its right side showed normal female characteristics, whereas the left side had normal male traits. Different from other gynandromorphic ticks containing 1 anus, this tick reported here had 2 complete anuses, and the anus of the male part had a single adanal plate.
Animals
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Chimera/*anatomy & histology/genetics
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China
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Female
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Ixodidae/*anatomy & histology/genetics
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Male
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Sheep
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Sheep Diseases/*parasitology
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Tick Infestations/parasitology/*veterinary
5.Practice of Pharmaceutical Care in Designated Hospital under the Condition of Novel Coronavirus Pneumonia Epidemic
Jingze CHEN ; Mingle HU ; Jiawei LUO ; Wei ZHANG ; Dingyu ZHANG
China Pharmacy 2020;31(5):536-539
OBJECTIVE:To shar e the experienc e of pharmaceutical care in Wuhan Jinyintan Hospital (herein after refers to “our hospital ”)under the condition of novel coronavirus pneumonia (COVID-19)epidemic,and to provide reference for other hospitals to deal with public health emergencies. METHODS :The situation of pharmaceutical care in our hospital under the condition of COVID- 19 epidemic was summarized and shared ,including the epidemic prevention and control management (regional division ,disinfection management ,pharmacy personnel training ),supply of drugs and disinfection products ,the monitoring and education of rational drug use by information technology. RESULTS :The pharmacy department of our hospital divided the activity scope into clean area ,potential pollution risk area ,semi pollution area ,and implement different disinfection management. All pharmacists received training ,involving personal health protection ,prevention and control knowledge of COVID-19,health status monitoring ,etc. For supply and guarantee of drugs and disinfectants ,the epidemic drug list of our hospital was formulated ,drugs and disinfectants were purchased accurately and stored in a standardized way. 24 h response telephone was set up in the clinical pharmacy room to receive consultation from clinicians on drug use at any time. The drugs mentioned in the COVID- 19 diagnosis scheme were compared in terms of the mechanism of action and the medication of special populations to form a tablet ,so as to help clinical rational choice treatment drug. CONCLUSIONS :The pharmaceutical care in the designated hospital of COVID- 19 is a professional and complicated work ,involving a wide range of aspects. Pharmacy department must respond actively and adjust the strategy in time so as to play an important role in improving the ability of medical treatment.