Object To establish the IR spectrometry fingerprint of the certified root bark of Morus alba L. (RBMA) and its confusable species, so as to identify them. Methods The infrared spectrometry was used. Results Three types of fingerprint were obtained from certified RBMA from different productive area and five types of fingerprint obtained from confusable species. These spectra were analyzed and the transmittance of (1 627?7) cm -1 of fingerprint area was taken as the standard absorbed peak (Ts) and the transmittance of (1 026?5) cm -1 of fingerprint area as the characteristic obsorbed peak (COP, Ti), the relative transmittance (Tr) was calculated by the formula Tr=Ti/Ts. Conclusion The tested samples of which Tr 1 and COP was single peak or Tr

Objective: To study the best gathering period and processing method of Cortex Mori.Methods: A HPLC method was used to determine the content of scopoletin. Results: The scopoletin contents in Cortex Mori picked in Jan., Feb. Apr., Jul. and Aug. were higher than that picked in the other months. The scopoletin content in Cortex mori of which rough barks were unremoved was higher than that of which rough barks were removed.Conclusion: Spring and Summer are the best gathering periods, but the processing method shouldn't be stricthy limited.

Object To research the effect of laurocapram upon the percutaneous absorption of Huayu Babu Sticking Plaster (HBSP) in vitro. Methods The improved Franz diffusing cells were chosen to study percutaneous absorption of piperine from HBSP. The content of permeated piperine was determined by HPLC; while the content of augenol was determined by GC to inspect the stability of HBSP. Results The result showed that the process of penetrating of piperine in HBSP through skin could be in accordance with zero-grade releasing equation and HBSP was stable during the course of experiment. Conclusion Laurocapram could promote percutaneous absorption of piperine.

AIM:To establish a method for GC fingerprint determination of the chemical constituents in the volatile oil of Eucalyptus globulus Labill.in Yunnan Province,China. METHODS: GC was used to optimize the fingerprint determination method,and to identify the main peaks in the GC fingerprint. RESULTS: 16 mutual peaks were gained on the base of 10 batches of samples. CONCLUSION: Stable and reproducible GC fingerprint is helpful to further analyze the volatile oil constituent of Eucalyptus globulus Labill.

Objective:To study the contents and determinate method of Sanggenon C of Cortex Mori in different sources.Methods:Sanggenon C was determined by HPLC. The mobile phase was methanol water(75∶25). Detection wavelength was at 280nm.Results:The average recovery was 96.94% with RSD = 1.72% and the linear range was 0.32～4.80?g. The highest content of Sanggenon C was 0.55% and the lowest content was 0.02% from different producing area. Conclusion: The method is accurate and reliable with good reproducibility.

Object To study specific TLC identification of the root bark of Morus alba L. (RBMA)Methods The ethanol extracts of RBMA and other confusable species were subjected to TLC in different development system. The TLC plats were examined under shortwavelength UV light or colored by FeCl 3 solution.Results A mixture of chloroform and methanol (5∶1) is used for development and ferric chloride solution is used to color, a specific purple spot of the certified RBMA can be found.Conclusion The specific spot showed with TLC can be regarded as the basis of identification of RMBA and it was separated and identified as sanggenon C.

Bacteremia is a common complication in patients with liver cirrhosis,and its incidence rises as the severity of liver disease increa-ses.Liver cirrhosis complicated by bacteremia will lead to poor prognosis.The research progress in liver cirrhosis complicated by bacteremia is reviewed from the following aspects:risk factors,diagnostic points,treatment methods,prevention,and prognosis.It is believed that ear-ly prevention and treatment of bacteremia have great significance for the prognosis of patients with liver cirrhosis.

OBJECTIVE To investigate antibiotic resistance and the prevalence of serotype of Streptococcus pneumoniae in Wuhan.METHODS Totally 152 strains of S.pneumoniae were collected to test the MICs of various antibiotics by agar dilution method according to the approved standard of NCCLS.Serotyping of S.pneumoniae was performed by using quelling reaction.RESULTS Among 152 strains of S.pneumoniae,65(42.76%) strains were resistant to penicillin(MIC≥0.12mg/L).94.08%,50.66%,41.45% and 11.18% of S.pneumoniae were resistant against the first(cefalexin),second(cefaclor) and third(cefaxime and ceftriaxone) generation of cephalosporins respectively.The resistance rates to other antibiotic agents,such as erythromycin,tetracycline,trimethoprim/sulfamethoxazole and chloramphenicol,were 84.21%,88.82%,89.47% and 18.42%,respectively.Strains that were resistant to levofloxacin and moxifloxacin were found both for 1.32%.Twenty serotypes were involved in 152 strains. The prevalent serotypes were 19(25.66%),23(19.08%),6(13.82%),15(7.24%)and 14(4.61%).Eight strains were remained for unable to serotype.All penicillin-resistant S.pneumoniae was included in serotypes 6,19 and 23.CONCLUSIONS The antibiotic resistance of S.pneumoniae is serious in Wuhan.Most of them are multi-resistant strains.Except for fluoroquinolones,ceftriaxone and chloramphenicol, most antibiotic agents have lost there activities against S.pneumoniae.The prevalent serotypes,especially of the multi-resistant strains,were 19,23 and 6.Pneumococcal polyvalent vaccine can well cover these serotypes.

Object To study the contents and the determinating method of scopoletin in Cortex Mori from different sources.Methods Scopoletin was determined by HPLC.The mobile phase was MeOH-H 2O-HAc (40∶60∶0.25).Detection wavelength was at 354 nm.Results The average recovery was 98.88% with RSD=1.91% and the linear range was 0.025 6—0.128 ?g.The highest content of scopoletin was 0.017 3% and the lowest content was 0.002% from different producing areas.Conclusion The method is easy, accurate, reliable with good reproducibility and high sensitivity.