Objective To explore the application effect of self-made micro-video in practical teaching of mental and psychological nursing.Methods Two classes in major Nursing enrolled in 2013 were selected randomly. A total of 51 students from Class 12 were designated as the experimental group, and were taught assisted by self-made micro-videos, while another 53 students from Class 11 belonged to the control group, and were taught assisted by traditional instructional video. The students were surveyed by questionnaire on knowledge awareness rate on mental disease prevention, regulated in mental health work index investigation and evaluation scheme, formulated by General Office of National Health and Family Planning Commission of the People's Republic of China, basing on which, teaching effect was compared.Results After practical teaching, total accuracy rate of case examination by students in the experimental group was 94.12% (compared with 39.22% before the practice), and in control group, 81.13% (compared with 33.96% before the practice). Scores of attitude towards mental disease in the experimental group were (54.39±4.68), and in the control group (35.15±7.69) (t=15.49, P<0.01).Conclusions Applying self-made micro-video in practical teaching of mental and psychological nursing can effectively improve accuracy rate of mental disease recognition of the students, correct their attitude towards patients with mental disorders, with satisfying effect in practice.

Entero virus 71 (EV71) causes hand, foot, and mouth disease (HFMD) and occasionally leads to severe neurological complications and even death. Scavenger receptor class B member 2 (SCARB2) is a functional receptor for EV71, that mediates viral attachment, internalization, and uncoating. However, the exact binding site of EV71 on SCARB2 is unknown. In this study, we generated a monoclonal antibody (mAb) that binds to human but not mouse SCARB2. It is named JL2, and it can effectively inhibit EV71 infection of target cells. Using a set of chimeras of human and mouse SCARB2, we identified that the region containing residues 77-113 of human SCARB2 contributes significantly to JL2 binding. The structure of the SCARB2-JL2 complex revealed that JL2 binds to the apical region of SCARB2 involving α-helices 2, 5, and 14. Our results provide new insights into the potential binding sites for EV71 on SCARB2 and the molecular mechanism of EV71 entry.
Amino Acid Sequence ; Animals ; Antibodies, Monoclonal ; chemistry ; genetics ; metabolism ; Binding Sites ; Cell Line ; Crystallography, X-Ray ; Enterovirus A, Human ; drug effects ; genetics ; growth & development ; immunology ; Fibroblasts ; drug effects ; virology ; Gene Expression ; HEK293 Cells ; Humans ; Immunoglobulin Fab Fragments ; chemistry ; genetics ; metabolism ; Lysosome-Associated Membrane Glycoproteins ; chemistry ; genetics ; immunology ; Mice ; Models, Molecular ; Protein Binding ; Protein Conformation, alpha-Helical ; Protein Conformation, beta-Strand ; Protein Interaction Domains and Motifs ; Receptors, Scavenger ; chemistry ; genetics ; immunology ; Receptors, Virus ; chemistry ; genetics ; immunology ; Recombinant Fusion Proteins ; chemistry ; genetics ; immunology ; Sequence Alignment ; Sequence Homology, Amino Acid ; Sf9 Cells ; Spodoptera ; Thermodynamics