BACKGROUND:Docetaxel, a cel cycle specific anti-tumor drug, is a drug that is used primarily for treating breast cancer; however, its efficacy is low when used for treatment of cancer not sensitive to radiotherapy. Bone marrow mesenchymal stemcels have been shown to strengthen the effects of tumor-specific targeting chemotherapy drugs. OBJECTIVE:To investigate the effects of docetaxel combined with bone marrow mesenchymal stem cels (BMSCs) on human hepatoma cel line SMMC-7721. METHODS:BMSC cels were culturedin vitro. The logarithmic growth of SMMC-7721 hepatoma cels were randomly divided into blank control group, BMSCs group and combined treatment group (combined treatment of BMSCs and docetaxel). SMMC-7721 cel cycle was detected usingflow cytometry. Cel growth rate of SMMC-7721 was determined by MTT assay. mRNA and protein expressions of tumor suppressor genes PTEN and p53 were detected by reverse transcription polymerase chain reaction and western blot assay. RESULTS AND CONCLUSION:Combined treatment of docetaxel and BMSCs inhibited SMMC-7721 cell proliferation. Compared with the blank control group, the number of cells at the G0/G1 phase was significantly increased in BMSCs group and combined treatment group. The cell growth rate of SMMC-7721 was significantly inhibited in BMSCs group compared with the blank control group, and that was further inhibited in combined treatment group (P< 0.05). mRNA and protein expressionof PTENandp53 were significantly increased in combined treatment group compared with BMSCs group and blank control group (P< 0.05). Our results suggest that BMSCs inhibit the growth of SMMC-7721 cells,andcombined use of docetaxel and BMSCs strengthensthe antitumor effect of BMSCs.

Objective To explore nursing points on the enteral nutrition support to batched patients with severe burn caused by explosion. Method An enteral nutrition support was carried out for 15 patients with severe burns admitted in our department and pertinent nursing was performed. Results Five patients died because of lack of autologous skin sources and complications of sepsis in 45~87 d after burn. Ten patients got a good recovery after enteral nutrition support, anti-infection treatment and nursing. They lived through the infection period, went into the recovery period. The hospital stay of these patients in ICU was 54~115 d with an average of (90.63 ± 30.37)d. Conclusion Early enteral nutrition support and nursing to the patients with severe burn can improve the nutritional status of patients effectively and promote the rehabilitation of patients.

Objective To investigate the effect of different dose dexmedetomidine on reducing agitation after sevoflurane anesthesia in patients underwent repair of indirect inguinal hernia.Methods 105 cases of ASA Ⅰ ～ Ⅱ received operation with inhalation of 3％ sevoflurane to induce anesthesia,and then randomly divided into three groups,35 cases in each group.The control group injected with 0.9％ sodium chloride,observation 1 group injected dexmedetomidine 0.15μg/kg,observation 2 group injected dexmedetomidine 0.30μ g/kg.Then placed oropharynx airway ventilation,caudal block.Anesthesia was maintained with 1.2％ sevoflurane,maintain its independent ventilation.Hemodynamic and respiratory changes of the children were recorded.Time to eye opening(TEO) at the end of anesthesia was also recorded.Results Three groups of children had similar operation process.TEO of the control group was (8.45 ± 4.02) min,observation 1 group was (9.23 ± 5.03) min,observation 2 group was (10.81 ± 4.06) min (t =1.23,1.64,all P ＞ 0.05).The incidence rate of agitation of the control group was 42.8％,that in the observation 1 group and observation 2 group was 20％,8.6％.Observation 1 group was significantly lower than that of control group (x2 =3.72,P ＜ 0.05),observation 2 group was significantly lower than that of control group and observation 1 group (x2 =5.14,4.27,all P ＜ 0.05).Conclusion The dose of dexmedetomidine 0.3 μg/kg administered after induction of anesthesia could reduce the postsevoflurane agitation in children and have no adverse effects.

Since the introduction of antiretroviral therapy(ART),the lifespan and quality of life of patients infected with HIV have been significantly improved.But treatment efficacy was compromised eventually by the development of resistance to antiretroviral drugs,and more new anti-HIV drugs with lower toxicity and higher activity were needed.Based on the experience and lessons learned from the treatment in the developed countries,US FDA suggested that more pharmacodynamical researches should be considered ahead of the clinical trials.To facilitate the anti-HIV drug research and development,we reviewed a few specialized issues that should be focused on drug evaluations in vitro,including: 1)Mechanism of action studies,demonstrating the candidate drug's efficacy to specifically inhibit viral replication or a virus-specific function and confirm the drug target.2)Drug resistance studies,selecting the drug-resistant variants in vitro and determining the activities inhibiting HIV isolates resistant to approved antiretroviral drugs of the same class.3)Antiviral activity in vitro in the presence of serum proteins,ascertaining whether an investigational product is significantly bound by serum proteins.4)Combination activity analysis,evaluating in vitro antiviral activity of an investigational product in two-drug combinations with other drugs approved.

To summarize the risk factor,pathogenic bacteria,medical and prevention progress of ventilator-associated pneumonia.It is beneficial to avoid the risk factor of ventilator-associated pneumonia,and to reduce morbidity and mortality.

This article summarizes the high risk factors and the progress of prevention in neonatal necrotizing enterocolitis.It is beneficial for early intervention and reducing the morbidity and mortality of neonatal necrotizing enterocolitis.

Objective To study the pre-and post-surgical orthodontic treatment methods of patients with skeletal malocclusion treated through combined orthodontic-orthognathic surgery,and to set some guidance for the future clinical Work.Methods Twenty-five skeletal malocclusion patients in our clinic aged from 17 to 36 were treated by presurgical orthodontic treatment,orthognathic surgery and postsurgical ortho dontic treatment.Presurgical orthodontic treatment included alignment of dental arches,leveling of arches,elimination of rotations,coordination of the arches,and decompensation of anterior and posterior teeth.The postsurgical orthodontic treatment included closing residual spacing,final tooth alignment,maximum interdigitation,finalizing torque,artistic positioning,ideal overjet/overbite relationship and establishment of correct root parallelism.Results The duration of presurgical orthodontic treatment was 14.41 months on average(2～23 months),and the postsurgical orthodontic treatment time was 6.94 months on average(1.5～13.5 months).The duration for the whole active treatment was 21.35 months on average(7～35 months).The maxilla was moved back on an average 5.00 mm in maxillary protrusion cases.The mandible was moved anteriorly on an average 7.25 mm in mandibular retrusion cases.The mandible was moved back on an average 6.55 mm in mandibular protrusion cases.The chin was moved anteriorly on an average 5.33 nan in retrusion of chin cases.Conclusions Pre-and post-surgical orthodontic treatments are important procedures to the surgical treatment for skeletal malocclusion and basic needs to obtain successful treatment results.

BACKGROUND: After tooth extraction, residual ridge resorption is a complicate process, while stress is one of the main causes. Recent studies have demonstrated that osteopontin (OPN) is a kind of agent that is sensitive to mechanical stress.OBJECTIVE: To study the role of OPN in mediating mechanical stress effect on bone, and to investigate the relationship of OPN and bone rebuilding, action mechanism of OPN and cell surface receptor and the relationship of OPN and osteoblast and osteoclast, in order to reveal the molecule mechanism of residual ridge resorption mainly caused by stress, and also to be a basic theory for clinical molecule therapy or gene therapy.RETRIEVE STRATEGY: PubMed was searched from January 1985 to June 2007 for English literatures with the searching words of "OPN, mechanical stress and bone resorption" and China Journal Full-text Database was also searched from January 1995 to June 2007 for Chinese literatures with the searching words of "OFN, mechanical stress and bone resorption". Fifty-two literatures were retrieved. The data were primarily selected. Inclusive criteria: being relative to role of OPN in mediating mechanical stress effect on bone. Exclusive criteria: repetitive studies.LITERATURE EVALUATION: Among the 52 literatures, including 6 Chinese and 46 English, 5 were rejected for objective independent from this study, 13 for repetitive contents, and so 34 literatures were involved for further analysis on the role of OPN in mediating mechanical stress effect on bone, 6 being review and the others being clinical or basic studies.DATA SYNTHESIS: ①After tooth extraction, residual ridge resorption is a complicate process. It also influences the effect of artificial denture, while stress is the main cause. ② OPN is widely distributed throughout the body, and has many physiologic functions. Recent studies have demonstrated that OPN is a kind of agent that is sensitive to mechanical stress. ③Bone is a special kind of biological tissue, bone resorption and bone rebuilding both can take place when it is lack of mechanical stress (atrophy of disuse),or endures mechanical stress. ④Bone rebuilding can be seen as the results of these molecule events. OPN, as a main protein, participates in the bone resorption and bone rebuilding. ⑤These OPN can be seen as a chemotatic factor to guide prosoma osteoclast to migrate to the surface of the bone, and mediate it to combine with the integrin on the surface of osteoclast so to promote the resorption of bone. So, OPN is a necessary factor in mechanical stress induced bone loss. ⑥OPN is a signal of transmission between osteoblast and osteoclast. ⑦OPN can be seen as a long acting feedback inhibition signal to inhibit bone mineralization.CONCLUSION: OPN is a kind of agent that is sensitive to mechanical stress. It plays an important role in mediating mechanical stress effect on bone.

Objective To establish a method for the determination of chlorogenic acid in Fuyanqing perfusate (FP). Methods The sample was extracted with methyl and the chromatographic condition were as follows: C18 chromatographic column (250 mm? 4.6 mm,5 ? m),a mobile phase of methyl alcohol and water and methyl acid(30 ∶ 70 ∶ 1),the detection wavelength at 327 nm and the flow rate being 0.5 mL/min.Results A linearity of chlorogenic acid in FP was obtained in the range of 0.16 ? g～ 0.58 ? g,r=0.9996(n=6).The average recovery was 98 % and RSD =1.0% (n=6).Conclusion This method is easy,sensitive and accurate for the determination of chlorogenic acid in FP.