Bacteremia is a common complication in patients with liver cirrhosis,and its incidence rises as the severity of liver disease increa-ses.Liver cirrhosis complicated by bacteremia will lead to poor prognosis.The research progress in liver cirrhosis complicated by bacteremia is reviewed from the following aspects:risk factors,diagnostic points,treatment methods,prevention,and prognosis.It is believed that ear-ly prevention and treatment of bacteremia have great significance for the prognosis of patients with liver cirrhosis.

Objective BREAST-Questionnaire (BREAST-Q) was a patient-reported outcome (PRO) instrument designed by the Memorial Sloan-Kettering Cancer Center (MSKCC) and University of British Columbia (UBC).Our study aimed to evaluate the Chinese version of BREAST-Q augmentation module in China.Methods BREAST-Q augmentation module consisted of two parts:pre-operative and post-operative.Pre-operative had 25 items.Post-operative had 88 items.BREAST-Q questionnaires were given to ten breast augmentation patients.All patients completed BREAST-Q questionnaires at two weeks pre augmentation,one day pre augmentation,fourteen weeks post augmentation,and sixteen weeks post augmentation.Results At two weeks pre augmentation,scores for satisfaction with breasts,psychosocial well-being,and sexual well-being were 29.9 + 5.8,16.5 ± 10.2,and 28.2± 9.3,respectively.At sixteen weeks post augmentation,scores for these three domains were statistically increased to be 65.3 ± 10.0,67.5±17.8,and 56.5±22.2,respectively.These differences were all above 20.However,there was no significant difference between pre augmentaion score and post augmentation score for physical well-being.Pre augmentation score was 64.9 ± 11.9,and post augmentation score was 56.0±14.5.Test-retest reliabilities in different domains were all above 0.8 and most were above 0.9.The Cronbach's alpha coefficients were higher than 0.6.Conclusions Chinese version of BREAST-Q augmentation module is reliable and responsive in assessing the quality of life in Chinese women with breast surgery.However,larger sample is needed for future study.

Objective:To explore the role of hippocampal γ oscillation abnormality in sepsis-associated encephalopathy (SAE).Methods:Seventy male Sprague-Dawley rats (2-3 months) were randomly (random number) divided into three groups according to the random digital table method: sham, CLP, and CLP + dopamine 4 (D4) receptor agonists RO-10-5824 group. The SAE animal model was established by cecal ligation and puncture (CLP). On day 10-14 after surgery, the open field, novel object recognition, and fear conditioning tests were performed. After that, the hippocampus was collected to measure expressions of parvalbumin (PV) and D4 receptor. In another set of experiment, CA1 local field potential (LFP) were recorded, and the relationship between LFP and time with novel object was analyzed. Independent sample t-test was used for pairwise comparisons, and multiple comparisons were performed by one-way ANOVA, followed by the Tukey multiple comparisons test. Correlation was analyzed using Pearson correlation. Statistical significance was assumed when P<0.05. Results:Compared with the sham group, hippocampal PV (77.54±4.61)%, D4 expression (56.36±3.88)% and γ oscillation power (41.1±8.62)%, object exposure time (36±3) s, new object recognition rate (49±4)%, and scene stiffness time (56±7) s were decreased significantly ( P<0.05). However, RO-10-5824 treatment could increase hippocaml γ oscillation power (92.3±6.7)%, and reverse the decreased new object exposure time (44±3) s and new object recognition rate (63±4)%. Correlation analysis showed that hippocampal γ oscillation power was positively associated with new object exposure time ( r=0.609 2, P=0.015 9). There was no difference in total distance traveled or time spent in the center among groups ( P>0.05). Conclusion:Hippocampal γ oscillation abnormality might play a key role in cognitive impairment associated with SAE.

Objective To evaluate the feasibility of 80 kVp tube voltage combined with adaptive statistical iterative reconstruction (ASiR)in low dose contrast media split-bolus 2-phase CTU.Methods Sixty patients with hematuria were recruited in this study.All the patients (80 kVp Group,n=30;120 kVp Group,n=30)underwent split-bolus 2-phase CTU (80 kVp Group:35 mL and 60 mL;120 kVp Group:50 mL and 70 mL).CTU images were reconstructed with 30％ ASiR in 80 kVp Group and with filtered back projection (FBP)in 120 kVp Group.Subjective and objective analysis of CTU images were accomplished by two qualified and independent readers.The radiation dose was evaluated by ED and SSDE.The image score,noise,attenuation value and CNR of urinary tract,and radiation dose were compared by Mann-Whitney rank sum test.Results There was no significant difference in the image quality between two groups (Z =-1.791,P =0.073).The noise of 80 kVp Group were higher than that of 120 kVp Group (Z =-6.299,P <0.001 ), while there was no significant difference of attenuation value between the two groups (Z =-1.204 --0.163,P =0.228 -0.871,except bladder),as well as the CNRs (Z =- 1.818 --0.202,P =0.069 -0.840).The ED and SSDE of 80kVp Group were significantly lower than those of 120 kVp Group (Z =-6.655--6.653,P <0.001).Conclusion The protocol of 80 kVp tube voltage combined with iterative reconstruction in low dose contrast media split-bolus 2-phase CTU is feasible for clinical application,with reduction of radiation dose and acceptable diagnostic image quality.

Objective: To improve the quality standard of Funing granules. Methods: TLC was used for the qualitative identifica-tion of Angelicae Sinensis Radix, Chuangxiong Rhizoma, Scutellariae radix, Atractylodis macrocephalae rhizoma, Citri reticulatae peri-carpium, Cyathulae radix, Paeoniae radix alba and Glycyrrhizae radix Et rhizoma. The content of paeoniflorin was determined by HPLC. Results: The TLC spots were clear and well-separated without any interference from the negative control. The linear range of paeoniflorin was 19. 23-2525. 18 ng(r=0. 999 9), the average recovery was 101. 88% ,and the RSD was 0. 62% (n=6). Conclu-sion: The method is simple and accurate with good reproducibility, and can be used for the quality control of Funing granules.

Objective Toinvestigatestatistically significant peptide peaks as biomarkersto diagnose osteoarticular tuberculosis, matrix-assisted laser desorption/ ionization time of flight mass spectrometry (MALDI-TOF MS) was applied to identify the characteristic fingerprint among the serum of patients with osteoarticular tuberculosis, rheumatoid arthritis and healthy adults.Methods Clinical Study. Serum samples of untreatedpatients with osteoarticular tuberculosis and rheumatoid arthritis were collected from August 2018 to December 2018, and serum samples of healthy adults from physical examination were collected as control. After analysis with MALDI-TOF MS, the serum peptide fingerprint datawas imported into software, and protein polypeptide peaks with obvious differences were screened to establish diagnostic models.Results Established the diagnostic model of osteoarticular tuberculosis and healthy adults with m/z 2943.9, 5929.6, 7615.4 and 9033.8 as differential protein polypeptides, the diagnostic model of osteoarticular tuberculosis and rheumatoid arthritis with m/z 4195.6, 5847.6, 5929.6 and 7748.6 as differential protein polypeptides. To these two models, the sensitivity were 95.00％ and 97.50％, respectively. The specificity were 85.71％ and 88.46％, respectively. The accuracy rates were 89.58％ and 92.39％, respectively. The AUC value of ROC curves were 0.8859 and 0.8709, respectively. Conclusions By mass spectrometry and software analysis, the serum protein polypeptides with statistical difference were found successfully. The related diagnostic modelsarealso established, which has certain reference value for auxiliary diagnosis of osteoarticular tuberculosis.

Objective:To study the non-target metabolomics analysis and to analyze the metabolomic changesof cerebrospinal fluid (CSF) in patients with tuberculous meningitis.Methods:Case-control study. From July 2018 to July 2019, 20 cerebrospinal fluid specimens of diagnosed patients with tuberculous meningitis were collectedin the department of neurology from the first medical center of the PLA general hospital and the eighth medical center of the PLA general hospital and 20 CSF without tuberculous meningitis as the control. Among them, there were 12 males and 8 femalesin the tuberculous meningitis group, aged (37.9±16.1) years; there were 13 males and 7 femalesin the control group, aged (34.7±14.8) years. Using ultra-high performance liquid chromatography-mass spectrometry (UPLC-MS) technology with three different mode, namely reverse phase chromatography positive ion mode, reverse phase chromatography negative ion mode and hydrophilic chromatography positive ion mode,to detectthe metabolic fingerprints of patients′CSF and analyzed by SIMCA software for orthogonal partial least squares discriminant analysis (OPLS-DA). The variable importance projection value of OPLS-DA model (threshold value>1) plus the P value of t-test (P<0.05) was applied to find the differential metabolites in the cerebrospinal fluid of the two groups of patients.Results:Ten differential metabolites were found in CSF, including L-isoleucine, L-phenylalanine, L-kynurenine, L-methionine, L-tyrosine acid, dimethylglycine, L-alanine, L-threonine, L-histidine and L-lysine, and all of them were up-regulated in the tuberculous meningitis group.Conclusion:Changesof the amino acid metabolism found in the cerebrospinal fluid of tuberculous meningitis patients can provide basis for differential diagnosis and basic molecular research of tuberculous meningitis.

Objective To analyze the expression profile of long non-coding RNA (lncRNA) in regressive scar and mature scar.Methods Hematoxylin-eosin staining and Masson staining were used to examine the histology of regressive scar and mature scar.The technique of IncRNA microarray was used to test the IncRNA expression profile in regressive scar and mature scar.The raw data of lncRNA were pretreated for normalization.Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was further performed.Four lncRNA expressions were validated by quantitative PCR.Results Compared with mature scar,more fibroblasts and deeper Masson staining color were seen in the regressive scar,a total of 1 275 upregulated more than 3-fold were found in the regressive scar.And 596 downregulated more than seventy percent lncRNA were found in the regressive scar.Pathway analysis indicated that chemokine signaling pathway,TNF signaling pathway,insulin secretion and focal adhesion would play important roles in scar development.Conclusions lncRNA expression profiles in the regressive scar is markedly different in comparison with the mature scar,revealing that lncRNA may participate in pathophysiological process of scar maturation.

Objective To analyze the expression profile of long non-coding RNA (lncRNA) in regressive scar and mature scar.Methods Hematoxylin-eosin staining and Masson staining were used to examine the histology of regressive scar and mature scar.The technique of IncRNA microarray was used to test the IncRNA expression profile in regressive scar and mature scar.The raw data of lncRNA were pretreated for normalization.Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was further performed.Four lncRNA expressions were validated by quantitative PCR.Results Compared with mature scar,more fibroblasts and deeper Masson staining color were seen in the regressive scar,a total of 1 275 upregulated more than 3-fold were found in the regressive scar.And 596 downregulated more than seventy percent lncRNA were found in the regressive scar.Pathway analysis indicated that chemokine signaling pathway,TNF signaling pathway,insulin secretion and focal adhesion would play important roles in scar development.Conclusions lncRNA expression profiles in the regressive scar is markedly different in comparison with the mature scar,revealing that lncRNA may participate in pathophysiological process of scar maturation.

As an important force in promoting the transformation and upgrading of the national economy, the occupational health of the Internet industry's employees has received widespread attention. This paper examines the current situation of occupational health risks and management in the Internet industry at home and abroad, and on this basis reveals the physical and mental health problems of Internet industry employees. Combining the existing regulations, policies and standard documents at home and abroad, countermeasures are proposed to improve the relevant laws and regulations and their guidelines, implement relevant job design requirements, build a healthy working environment, optimise the work management system and carry out workplace health promotion, in order to provide ideas to accelerate the improvement of occupational health issues in the Internet industry.