Objective To compare the effects of fallopian tube recanalization and hydrotubation for treating partially obstructed fallopian tubes .Methods A retrospective analysis was conducted in 464 patients with partially obstructed bilateral fallopian tubes diagnosed by hysterosalpinography ,excluding other non -tubal infertility patients ,in the intervention department of our hospital Hospital from Jan .2005 to Dec .2010 .The patients were divided into 3 groups:fallopian tube recanalization group (157 cases) , hydrotubation group (155 cases) and control group(152 cases) .Results The intrauterine pregnancy rate of the fallopian tube reca-nalization group was higher than that of the hydrotubation group and control group .The ectopic pregnancy rate of the fallopian tube recanalization group was lower than that of the hydrotubation group and control group .The intrauterine pregnancy rate of the hydrotubation group was higher than that of the control group ,there was statistical difference between the two groups (P<0 .05) . While there was no statistical difference in the ectopic pregnancy rate between the hydrotubation group and the control group (P>0 .05) .Conclusion Active medical intervention can make the clinical benefit in the patients with partially obstructed fallopian tubes ,moreover ,fallopian tube recanalization is better than hydrotubation in the curative effect .

Colorectal cancer(CRC) is one of the most common tumors with a high prevalence of cancer-related morbidity and mortality.Several environmental factors,especially dietary factors,can affect the initiation and development of CRC.Recent studies have shown that adjusting nutrition components in diet,such as folate,calcium,vitamin D,selenium and fibre,may help to prevent the development of CRC.The objective of article is to review mechanisms of these nutritional components in the process of preventing CRC.

Objective To compare three different curative effects on hydrosalpinx before in vitro fertilization and embryo transfer (IVF-ET). Methods Patients receiving IVF-ET between January 2011 to December 2013 (n=731) due to tubal factor infertility (hydrosalpinx) were retrospectively analyzed. All patients were divided into four groups. Embolization group underwent fallopian tube embolization (n=257). Colostomy group underwent laparoscopic tubal umbrella end colosto?my (n=193). Excision group underwent laparoscopic tubal excision(n=198). Control group did not undergo any effusion be?fore transplantation (n=83). Results Comparing main indicators of ovarian hyperstimulation (the number of antral follicles, the dosage of gonadotropin (Gn), the number of retrieved oocytes), indicators were better in embolism group, colostomy group and control group than those in excision group. Comparing main indexes of IVF-ET (embryo implantation rate, clinical preg?nancy rate, abortion rate), indicators were better in embolism group, excision group than those in colostomy group while indi?cators in colostomy group were better than those in control group. The pregnancy rate in fallopian tube was the lowest in em?bolism group and excision group, and the highest in control group. Conclusion All three methods of processing hydrosal?pinx before transplantation ended with positive impact on IVF-ET, and fallopian tube embolization has certain advantages over the other two treatment measures.

LEEP electro-scalpel is a type of cervical disease device. It belongs to the high frequency electro-knife in medical apparatus category. There is no domestic LEEP electro-scalpel at present. It is significant and important for the study group to resolve the safety and EMC problem and develop domestic LEEP electro-scalpel which is safe, stable and competitive in price while has the same surgery effect as the imported one.

Since the HLA system is one of the most complex human genetic polym- orphisms,its application in forensic medicine included disputed paternity and criminal identification,have been fairly recognized. The present paper reported the results of our study about the HLA typing in human blood stain,serum and saliva,it was concluded that:(1).The existed strong anti-complementary activity in human blood stain when the amount of complement used in microlym-phocytotoxicity inhibition test(MLIT) was incresed to 10?l,it was found that the results of HLA-All,-B 5 typing in bloodstains were all correct,and the detectable period was at least 90 days; (2).The soluble HLA-A antigens in human serum could reliable detected with MLIT;(3).The soluble HLA-A antigens were also present in the human siliva.

Objective To investigate the effects of DNA methylation and histone acetylation on cell cycle progression and expression of tumor suppressor genes in human colonic cancer cells. Methods Three colonic cancer cell lines(HT 29,SW1116 and Colo 320) were treated with the DNA methylation inhibitor, 5 aza 2' deoxycytidine(5 aza dC) and/or the histone deacetylase(HDAC) inhibitor, trichostatin A(TSA)and sodium butyrate. The methylation status of the promoter of the p16 INK4A gene was assayed by methylation specific PCR(MSP). The expression of p16 INK4A and p21 WAF1 were analyzed by RT PCR. Cell cycle distribution was studied by flow cytometry. Results p16 INK4A expression was weakly detected in three colon cancer cells (HT 29,SW1116 and Colo 320) and p21 WAF1 expression was not detected in SW1116 and Colo 320 cells before treatment. The methylation status of the promoter of the p16 INK4A gene was significantly decreased and mRNA expression was markedly increased in HT 29 cells after treatment with 1 ?mol/L but not 10 ?mol/L of 5 aza dC for 24 hours. In SW1116 and Colo 320 cells, the expression of p16 INK4A was obviously enhanced at 10 ?mol/L or 5 ?mol/L of 5 aza dC for 24 hours. However, p21 WAF1 gene expression has not been detected. Interestingly, after treatment of TSA or sodium butyrate, the transcription of p21 WAF1 was significantly up regulated in these two cell lines. In addition, 5 aza dC did not affect cell cycle distribution, but TSA or sodium butyrate blocked cells mainly in the G 1 phases. Conclusions The expression of p16 INK4A gene was regulated by DNA methylation in three human colonic cancer cells. The expression of p21 WAF1 gene was regulated by histone acetylation in SW1116 and Colo 320. In these two cell lines, histone hyperacetylation causes a G 1 cell cycle arrest.

BACKGROUND:The main therapy of severe aplastic anemia in children is immunosuppressive therapy or stem celltransplantation, but the latter one is restricted due to few donor sources. Haploidentical hematopoietic stem celltransplantation is commonly used in leukemia, but it is stil rarely reported in the treatment of aplastic anemia. OBJECTIVE:To investigate the effect of haploidentical hematopoietic stem celltransplantation combined with placenta-derived mesenchymal stem celltransplantation for children with severe aplastic anemia. METHODS:A 7-year-old girl who had been confirmed as having severe aplastic anemia for 1.5 years received a cotransplantation of haploidentical hematopoietic stem cells combined with placenta-derived mesenchymal stem cells on July 9th , 2012. The donor was her mother. The preconditioning regimen consisted of fludarabine, cyclophosphamide, and anti-thymocyte globulin. RESULTS AND CONCLUSION:Time of neutrophil recovery (>0.5×10 9/L) was+9 days, and hematopoietic reconstruction was complete at+12 days. The short tandem repeat analysis showed 100%donor’s genotype at+100 days. Immunosuppressive drugs were stopped at+8 months, and no acute or chronic graft-versus-host disease occurred. With a fol ow-up of 18 months, she was in the disease-free survival period. Our findings suggest that the cotransplantation of al ogeneic haploidentical hematopoietic stem cells and placenta-derived mesenchymal stem cells is a new effective approach for children with severe aplastic anemia, which is worth exploring in the future.

Objective To investigate the combined inhibition effect and the potential mechanism of rapamycin (mammalian target of rapamycin inhibitor) and PD98059 (mitogen-activated protein kinase/extracellular signal-regulated kinase kinase (MEK) inhibitor) on mouse colorectal cancer (CRC). Methods S-ICR mice were subcutaneously injected with 20 mg/kg of 1,2-dimethylhydrazine dihydrochloride in the nape for 20 weeks to induce CRC. From the 16th week, the mice were treated with alone or combined injection with 0.25 mg/kg rapamycin and 2.5 mg/kg PD98059. The drugs were administered for 8 weeks. Subsequently, the animals were sacrificed and dissected, the tumor sizes were measured, and the tumors were harvested for pathological assay. Furthermore, the phosphorylation of mTOR, p70S6K, and 4E-BPl proteins was detected by using immunohistoehemistry. Results The mice treated with rapamycin (44. 44 %) or PD 98059 (either alone (38.89%) or combination treatment (6.67%) were significantly less likely to develop cancer compared with mice receiving none of them (77.78%, P<0. 05). The average size of tumors was (6.15±2. 192), (8.85±3. 983), (2.917±0. 191), (16.36±6.855) mm3 respectively (P<0.05).The anti-cancer effect of the combination treatment was substantially significant. The proteins of phospho-mTOR, phospho-p70s6K and phospho-4E-BPl were significantly down-regulated after treatments (all P values < ,0.05). Conclusions Combined treatment was more effective than single-drug treatments of rapamycin or PD98059 alone for the prevention and treatment of mouse CRC. The mTOR signal pathway might be involved in the inhibitory mechanism.

BACKGROUD: Coronary artery disease (CAD), a common disease with high incidence and mortality rate, has seriously threatened the health and life of the public. Traditional Chinese medicine (TCM) has an important role in the prevention and treatment of this disease. Through clinical epidemiological survey, a deeper understanding of TCM etiology and syndrome characteristics in CAD would further improve clinical efficacy in the treatment of this disease.

DNA barcoding is a technique in which species identification and discovery are performed by using short and standard fragments of DNA sequences. In this study, eleven species of Paris, including seven varieties, were sampled. Five chloroplast sequences, psbA-trnH, rpoB, rpoC1, rbcL, matK, and one nuclear marker, the second internal transcribed spacer (ITS2) of ribosomal DNA, were amplified and sequenced. The PCR amplification and sequencing efficiency, intra- and inter-specific divergence and barcoding gap were used to evaluate different loci, and the identification efficiency was assessed using BLAST1 and Nearest Distance methods. The ITS2 sequences in the studied samples of Paris were amplified and sequenced successfully using primers designed by our group, while matK showed low level in the amplification and psbA-trnH was difficult for sequencing because of over 800 bp and poly (A) structure. Analysis of the intra- and inter-specific divergence and barcoding gap showed ITS2 was superior to other loci. The ITS2 showed a much higher percentage of success (100%) in identification than other five loci, none of which indicated more than 50% except matK (52.9%). The 2-locus combination of rbcL+matK didn't improve ability of authentication. In addition, the rate of successful identification with ITS2 kept 100% when the samples were expanded to 67 samples of 29 species. In conclusion, ITS2 can be used to correctly identify medicinal plants of Paris, and it will be a potential DNA barcode for identifying medicinal plants of other taxa.