Object: To explore the effects and the influent agents of early education on the healthy termed infants. Methods: 52 healthy termed infants voluntary to the exercise center as experimental group were early educated. The other 52 infants as control group were in the same elementary conditions comparing to the experimental group. The experimental group was trained the corresponding projects and examined DQ at the beginning and the 3 rd,6 th,9 th month after training. The control group was only examined DQ at the beginning and after 9 months. Results:①At the 9 th month after training, DQ of the experimental group was improved 21.9?13.6, which was much 7 times higher than that of the control group, which was improved 3?3.0 (P

90 ). ③For the experimental group at baseline, DQ of the gross movement was the highest (71.5?10.5), while that of the other four areas were similar (fine movement 56.7?10.4, adaptability 59.6?12.5, language 54.3?11.7, social 56.8?14.7).Conclusions:Early intervention had significant effects for the development of children with mental retardation, which medicine has less role.

Balb/c mice were immunized by intraperitoneal iniection of group A erythrocytes. Immunized spleen cells were fused with Sp2/0 murine myeloma cells from the solid tumour. After 10～15 days of incubation at 37℃ CO_2, the supernatants were screened for the agglutinabal antibodies. Three hybridoma cell lines secreting high titer and specific monoclonal anti-A antibodies were established.These hybridoma cells have the ability of constant seceting antibodies which belong to the IgM class. The specificity monocloal anti-A sera was the same as the human anti-A sera.

Objective To understand the molecular mechanism by which protein kinase C? mediates multidrug resistance of human glioma cell line SHG-44.Methods SHG-44/ADM was constructed by stepwise concentration increasing method and intermittent administration method.SHG-44/WT and SHG-44/ADM were treated by PKC? reactivator PMA and PKC? inhibitor staurosporine,then the expressions of PKC? and MDR-1 were detected by Western blotting,the PKC? activity was assayed by kinase,and ADM accumulation was determined by fluorescence spectrometry.Results PMA increased PKC? activity and MDR-1 protein expression and activity.Staurosporine was able to block PKC? activity and decrease MDR-1 expression and activity.Conclusion Multidrug resistance in human glioma cells is mediated by PKC? via MDR-1 pathway.

Objective To systematically review the efficacy and safety of photodynamic therapy (PDT) and intravitreal vascular endothelial growth factor (VEGF) inhibitors in the treatment of polypoidal choroidal vasculopathy (PCV),and to investigate the primary treatment tentatively.Methods A systematic search of Pubmed,Embase,the Cochrane Library and the Wanfang Data was performed to identify all comparative studies that compared the outcomes of PDT alone,intravitreal VEGF inhibitors alone and combined intravitreal VEGF inhibitors and photodynamic therapy.Outcomes of interest included the regression and recurrence rate of polypoidal lesions,best corrected visual acuity (BCVA),central retinal thickness (CRT),therapeutic times,and the occurrence rate of adverse events.2 randomized controlled trials (RCT) and 19 non-RTCs were identified.According to treatment methods,the data extracted was classified to 3 groups,analyzed with odds ratio (OR),weighted mean difference (WMD) and 95％confidence interval (95％CI).Results Meta-analysis suggests that the regression rate of polypoidal lesions (OR=0.34,0.07;95％CI=0.13-0.88,0.02-0.36) and BCVA (WMD=0.25,0.11;95％CI=0.14-0.36,0.01-0.21) in combined therapy group were significantly better than those in PDT group and intravitreal VEGF inhibitors group (P＜0.05).The recurrence rate of polypoidal lesions in PDT group was significantly lower than intravitreal VEGF inhibitors group (OR=0.35,95％CI=0.16-0.74,P=0.006).BCVA (P=0.025) and the occurrence rate of adverse events (OR=60.36,95％CI=6.04-603.50,P=0.000 5) in intravitreal VEGF inhibitors group were significant better than PDT group.Conclusions Combined treatment appeared to be superior to PDT alone or intravitreal VEGF inhibitors alone.Combined treatment takes priority over all others in the primary treatment of PCV.

One step dot-ELISA method for the rapid determination of ABO blood groups in humam body fluids(or stains)was established using enzyme-labelled anti-A,--B and anti--H monoclonal antibody (McAb).The sample was applied on the nitrocellulose membrance as a dot. After washing, the appropriate McAb wasadded on top of the dot, and then followed by adding 3, 3'-diaminobenzidine(DAB). The brown color indi-cated the positive reaction. The ABO blood typing of 521 saliva samples including both secretor and non-se-cretor were carried out. All the results were correct. The advantages of this method are accurate, sensitive,rapid, easy to perform, as well as not time consuming. It is more sensitive than the conventional hemaggluti-naton inhibition test.

Objective To investigate the effects of DNA methylation and histone acetylation on cell cycle progression and expression of tumor suppressor genes in human colonic cancer cells. Methods Three colonic cancer cell lines(HT 29,SW1116 and Colo 320) were treated with the DNA methylation inhibitor, 5 aza 2' deoxycytidine(5 aza dC) and/or the histone deacetylase(HDAC) inhibitor, trichostatin A(TSA)and sodium butyrate. The methylation status of the promoter of the p16 INK4A gene was assayed by methylation specific PCR(MSP). The expression of p16 INK4A and p21 WAF1 were analyzed by RT PCR. Cell cycle distribution was studied by flow cytometry. Results p16 INK4A expression was weakly detected in three colon cancer cells (HT 29,SW1116 and Colo 320) and p21 WAF1 expression was not detected in SW1116 and Colo 320 cells before treatment. The methylation status of the promoter of the p16 INK4A gene was significantly decreased and mRNA expression was markedly increased in HT 29 cells after treatment with 1 ?mol/L but not 10 ?mol/L of 5 aza dC for 24 hours. In SW1116 and Colo 320 cells, the expression of p16 INK4A was obviously enhanced at 10 ?mol/L or 5 ?mol/L of 5 aza dC for 24 hours. However, p21 WAF1 gene expression has not been detected. Interestingly, after treatment of TSA or sodium butyrate, the transcription of p21 WAF1 was significantly up regulated in these two cell lines. In addition, 5 aza dC did not affect cell cycle distribution, but TSA or sodium butyrate blocked cells mainly in the G 1 phases. Conclusions The expression of p16 INK4A gene was regulated by DNA methylation in three human colonic cancer cells. The expression of p21 WAF1 gene was regulated by histone acetylation in SW1116 and Colo 320. In these two cell lines, histone hyperacetylation causes a G 1 cell cycle arrest.

Codon usage bias is an important characteristic of genetic information transfer in organisms. Analysis of codon usage bias of different species is important for understanding the rules on genetic information transfer. The previous method for analysis of codon usage bias is mainly based on genomic data. However, this method is greatly limited, because the genome sequences of higher organisms are still not available up to now. In this study, we found that we could obtain the same optimal codons of Ganoderma lucidum (Curtis: Fr.) P. Karst based on its whole genomic data or large-scale transcriptomic data from its liquid-cultured hyphae, primordium and fruiting body, separately. This result indicated the feasibility to understand the codon usage bias based on the large-scale transcriptomic data. By calculating the proportion of rare codons of Escherichia coli and Saccharomyces cerevisiae in 26 terpene synthases (TS) of G. lucidum, we found that the rare codons of S. cerevisiae have a higher proportion in TS genes, while the rare codons of E. coli have relatively lower, suggesting that the TS genes of G. lucidum are possibly more difficult to be expressed in S. cerevisiae than in E. coli. Chemical synthesis of TS genes according to the yeast optimal codons will be an effective way to solve the problem on the mismatch of gene codon bias between the foreign genes and the host strain.

RNA-Sequencing (RNA-Seq) is a newly-developed method in transcriptome research, it can afford more accurate transcription information and be more quickly by using Next-generation Sequencing (NGS) technology. RNA-Seq has been widely used in various biological fields. Genuine traditional Chinese medicines (TCM), with good quality and therapeutic effect, were always praised highly and used by famous physicians. The geo-herbalism formation of TCM is based on the product of the gene expression at specific space and time. So it has been a research hotspot to analyze the mechanism of biosynthesis through RNA-Seq in the study on the secondary metabolism of medicinal plant. This article mainly illustrates the RNA-Seq and its advantages, it also discusses the potential application in genuine TCM, and it can provide useful information for other researchers.