The chemical constituents of the gorgonian Isis minorbrachlasta have been studied for the first time. Th?marine specimen was collected off the Nansha Islands in the South China Sea . On the basis of spectral correlation (1H NMR,13C NMR,IR)four known compounds:gorgosterol,22 - epi-hippuristanol, ceramide and batyl alcohol, were indentified.

PURPOSETo evaluate the effects and toxicites of combination chemotherapy of taxol, cisplatin and 5-flu-ouracil for the patients with advanced cancer. METHODS 7 cases /19 courses were administered by systematic chemotherapy, Taxol was given 150 mg-180 mg by intravenous (i. v.) infusion on day 1. cisplatin 40 mg i. v. on days 2 - 4. 5-fluouracil 750mg i. v. on days 2 - 4. 5 cases /13 courses were administered by intraperitoneal chemotherapy . Taxol was given 150-180 mg by intraperitoneal(i. p.) infusion on day 1. cisplatin 60 mg-100mg i. p. on day 1, 5-fluouracil 1000 mg i. p. on day 1. Course was repeated every four weeks. RESULTS Two patients showed complete response (16. 67%), eight patients showed partial response (66. 66%). the overall response rate was 83. 33%. The major toxic effects were myelosuppression, alopecia, arthralgra and myalgia. CONCLUSIONS Taxol, cisplatin. 5-fluouracil regimen is an effective treatment for patients with advanced cancer with acceptable side-effects.

Objective To construct eukaryotic expressing vector of the mouse NMNAT1 (nicotinamide mononucleotide adenylyl-transferase) gene and examine its ability to express the NMNAT1 gene in Hela cells.Methods The full-length NMNAT1 cDNA sequence was amplified by PCR and cloned into the plasmid of T-vector and then to pcDNA3.1 construct.The recombinant plasmid pcDNA3.1-NMNAT1 was identified by DNA sequencing and then transfected with Lipofectamine2000 into Hela cells.The expression of NMNAT1 was detected by real time quantitative PCR (qPCR) and Western blot after 48 h transfection.Results The recombinant eukaryotic vector carrying NMNAT1 gene was constructed successfully in a match with database and this vector could up-regulate the expression of the NMNAT1 gene both in mRNA and protein levels in Hela cells.Conclusions The eukaryotic vector carrying NMNAT1 gene (pcDNA3.1-NMNAT1) enhances the expression of NMNAT1 gene.

The current status of otolaryngology at the undergraduate stage teaching is:Too many curriculum contents,few theoretical and practical courses,students' lack of interest in study because they feel the contents difficult and so on.According to these situations,teaching reform has been carried out.The curriculum resource is divided into content-based curriculum resources and conditional resources.The current undergraduate teaching materials(Otolaryngology Head and Neck Surgery by People's Medical Publishing House,the Eighth Edition) is taken as its entry point,based on the current curriculum resources and its application situation in the teaching process.The study combines the teaching contents and curriculum resources and further explores the potential of curriculum resources and enlarges the resource space in order to explore the way and the method of using curriculum resource reasonably and efficiently,and construct the curriculum resources which have otolaryngology subject features and then guide teaching better.

OBJECTIVE:To provide reference for ADR evaluation in China.METHODS:The ADR signal evaluation method adopted by the pharmacovigilance center in Netherlands was analyzed by means of literature review,contrasting analysis and data reduction etc.RESULTS & CONCLUSION:We should draw experience from the pharmacovigilance center in Netherlands about the evaluation method on ADR signal and establish ADR evaluation databases of high quality;furthermore, we should attach great importance to the application of pharmacoepidemiology and statistics in ADR evaluation and gradually replace the qualitative evaluation with quantitative one for ADR signal.

OBJECTIVE: To analyze and evaluate the quantitative evaluation of ADR signals in FDA and to provide a reference for ADR evaluation in China. METHODS: By means of literature review,contrasting analysis and data reduction,ADR signal evaluation in FDA was investigated. RESULTS&CONCLUSION: In USA MGPS method is used for the quantitative evaluation of ADR signal, which can earlier obtain ADR signal and effectively promote the risk warning ability of ADR information. This is practical in our country and we should learn from them. Combined with various data analyzing methods, we can set up a high-quality evaluation database for ADR signals. The importance of pharmacoepidemiology and statistics in ADR evaluation should be highly recognized and the methods for ADR signals evaluation should be gradually shifted from qualitative to quantitative evaluation.

Objective To evaluate the expression of cytoplasmic CD_ 79a(CyCD_ 79a)on different acute leukemia(AL)cells and to explore its significance.Methods CyCD_ 79aexpression on the leukemic cells from 221 acute leukemia patients diagnosed from January 2003 to April 2004 was retrospectively analyzed by three-color cytometry with CD_ 45/SSC gating strategy.Results CyCD_ 79awas positive in 100%(57/57)of B cell-acute lymphoblastic leukemia(B-ALL)cases,which was higher than that of other B cell-associated antigens(CD_ 10,CD_ 19,CD_ 20and cytoplasmic CD_ 22)in B-ALL.Only 0.7%(1/134)of acute myeloid leukemia(AML)cases and 13.3%(2/15)of T cell-acute leukemia(T-ALL)cases expressed CyCD_ 79a,respectively.Conclusion CyCD_ 79ais sensitive and specific for B-ALL.CyCD_ 79amay be used as a reliable immunophenotyping marker for the diagnosis of B-ALL and be used to distinguish B-ALL from T-ALL and AML.

During the last forty years, although cytotoxic chemotherapy for gastric cancer patients has improved to some extent, its therapeutic effect is limited. Simultaneously, great progression of oncobiology to increase our understanding of the molecular basis of gastric cancer has been made. Currently the cancer cell has been characterized by several genetic changes that lead to altered cellular functions. In addition, multiple cancer-cell environment factors affect the tumor cell via various receptors and subsequent signaling pathways. The increasing knowledge of cellular signaling offers the opportunity to develop novel substances that target specific pathway molecules.

based on the theory of tree structure and followed the characteristics of medical talents growth,Wuxi No.2 People's Hospital carried out hospitalTalent Tree Project innovatively,which is to trained talents corresponding tobase-trunk-crown of a tree.The project has won the 2013 Human Resource Development Excellence Award of Asian hospital management awards.We fostered talents hierarchically according to the talents training echelon structure and this provided a way of guarantee and innovation for the sustainable development of hospital.

Objective To analyze the distribution characteristics and influencing factors of brachial-ankle pulse wave velocity (baPWV) in elderly population in order to direct the early diagnosis and prevention of arteriosclerosis in the elderly.Methods A total of 927 cases of elderly residents were randomly selected from 19 villages in Shengze area,Jiangsu Province.The indexes such as age,gender,height,weight,waist circumference,hip circumference and blood pressure were collected through standardized questionnaire and physical examination.The biochemical indicators including serum bilirubin,creatinine,uric acid,fasting blood glucose and blood lipids were measured,and baPWV was tested by automatic arteriosclerosis measurement system.Results The differences in age,systolic blood pressure,heart rate,serum bilirubin,creatinine,uric acid,triglycerides levels and baPWV were statistically significant between the male and female (all P＜0.05).BaPWV rised with the increase of age.Overall,baPWV was faster in the female than in male (P＜0.01).Blood pressure (r=0.36,0.59,P＜0.001),age (r=0.26,0.30,P＜0.001) and heart rate (r=0.30,0.33,P＜ 0.001) were correlated with baPWV,while uric acid had a correlation with baPWV (r=0.12,0.21,P＜0.01).A correlation was found between total cholesterol and baPWV (r=0.09,0.11,P＜0.05) Multi-variable linear stepwise regression analysis showed that systolic blood pressure,heart rate and age were the influencing factors for baPWV,of which systolic blood pressure had the greatest influence on baPWV (β＞0.5,P＜0.001).Conclusions With the increase of age,the baPWV shows rising trend.The monitor of baPWV may be useful in the elderly,especially in females.Blood pressure control,especially the control of systolic blood pressure can slow the progression of atherosclerosis.