Objective To observe the effect of curcumin combined with cisplatin on the proliferation of human tongue squamous cell carcinoma Tca?8113 cells,and explore the possible mechanism of their anti?tumor effect in vitro. Methods In this study,the samples were divided into four groups:curcumin,cisplatin,curcumin combined with cisplatin,and control. Detection of rate of inhibition of Tca?8113 cell proliferation was carried out by MTT assay. This was followed by observation of the morphological changes of the nuclei by Hoechst 33258 fluorescence staining. Subse?quently,cellular apoptosis was assayed by flow cytometry,and expression of Notch1 and epidermal growth factor receptor(EGFR)was examined by Western blotting. Results Results of MTT assay showed that curcumin combined with cisplatin inhibited the proliferation of tongue squamous cell carcinoma to a greater extent than either curcumin or cisplatin alone(P<0.05). Flow cytometry analysis indicated that,unlike curcumin or cisplatin alone,curcumin combined with cisplatin noticeably induced apoptosis(P<0.05). Results of Western blotting revealed that the expres?sion of Notch1 was upregulated,whereas the expression of EGFR was downregulated to a greater extent in the control group than in cells treated with curcumin or cisplatin alone. Unlike curcumin and cisplatin groups,the combined group revealed statistically significant expressions of Notch1 and EGFR(P<0.05). Conclusion Curcumin and cisplatin have a combined effect on inhibition of proliferation of human tongue squamous cell carcinoma Tca?8113 cells. The mechanism may be related to upregulation of the Notch1 signaling pathway and downregulation of the EGFR signal?ing pathway.

Objective:To study the exogenous oxidative stimuli on the mtDNA damage and proliferation of oral cancer SCC-25 cells.Methods:SCC-25 cells and fibroblast cell line BJ cells were treated by H2O2 at 0 (the control),120,240,480 and 960 μmol/L respectively for 15 m-24 h respectively,mtDNA damage was measured by a supercoiling-sensitive qPCR method(ss-qPCR),cell proliferation was tested by MTT assay.Results:Dose-dependent mtDNA damage was observed in SCC-25 cells treated by H2O2 at 120 μmol/L or above,and in BJ cells by H2O2 at 240 μmol/L or above,the mtDNA damage rate of both cells decreased 2-24 h after H2O2 treatment,in the 120 μmol/L group the damage rate reached to the control group.BJ cells showed less reaction to H2O2 stimulation than SCC-25 cells.Conclusion:Oxidative stress may be related to the development of oral cancer.

BACKGROUND: There is no systematical report on the conserved structure, protein characteristics and phylogenetic relationship of the Fasciclin gene family. OBJECTIVE: To investigate the evolutionary history of the Fasciclin gene family, and to systematically analyze the conservative structure, protein characteristics, and phylogenetic relationships of this gene family METHODS: Based on the published genome data of eight species such as people, chimpanzee and zebrafish, HMM models were established using the HMMER software to analyze the characteristics and genetic structure, structure domain and biochemical characteristics, evolutionary relationships and expression characteristics of the Fasciclin gene family. RESULTS AND CONCLUSION: In the eight species of fish (zebrafish), amphibian (xenopus), bird (chicken), reptile (Trionyx sinensis), mammal (rat, mouse) and primate (chimpanzee, human), 5, 5, 4, 5, 5, 4, 4, and 4 fasciclin candidate genes were retrieved respectively. Through the phylogenetic analysis of the fasciclin gene in these eight species, the evolutionary tree of fasciclin gene families can be divided into four evolution branch. The two branches of all are more conservative, likely to be the main osteoblast specific factor; the third branch may be osteoblast specific factor of amphibians, mammals and primate; and the final branch also has a good conservative, but the Xenopus and mouse in the branch has two copies of the genes, and this branch may play an important role in the evolution of these two species. Overall, in the evolution of species, the gene family has no larger changes, and such genes may be important for the growth and development. The lack of such genes may lead to the death of the individual or the extinction of species.

The rural health service is the important part of China’s health initiative and improving the rural grass-root health technical capabilities and service level marks the strategic initiatives and present needs to promote the rural health service development. The Traditional Chinese Medicine ( TCM) has a broad and solid mass base in rural areas and concentrating on the promotion of the TCM’s appropriate technologies constitutes an important way to strive for the rural health services development. Gangu and Jingning Counties of the Gansu province fully use the Health XI Project platform to promote the TCM’s appropriate technology application and explore the service model. With the achieved good experiment results, effective development of the TCM services is promoted.

Objective To explore the effects of group sand play on the behavior of cerebral palsy (CP)children with behavioral problems.Methods Ninety children with CP aged 3-5 with behavioral problems were selected,after those with mental retardation had been excluded through the Peabody picture vocabulary test (PPVT).Their behavioral problems were diagnosed using Achenhach's child behavior check list (CRCL).The children were randomly divided into an experimental group and a control group with 45 cases in each.The experimental group received 10 weeks of sand play treatment in addition to routine rehabilitation.The controls received only routine rehabilitation.Results After treatment,the social withdrawal,depression,aggression and disruptive behavior scores of the 3-year olds in the experimental group were significantly lower than before treatment and significantly lower than those of the controls.For the 4 and 5 year-old boys the results were similar in terms of soclal withdrawal,depression,immaturity,sexual behavior,aggression and delinquent behavior,For the 4 and 5 year-old girls the scores for depression,somatic complaints,social withdrawal,compulsion,aggression,and hyperauctivity showed similar significant improvements.Conclusion Group sand play can improve the behavior and promote the mental health of CP children.

Objective To explore the value of three-dimensional DSA (3D-DSA) in displaying the location of the origin of uterine artery.Methods A total of 90 female patients underwent uterine artery (UA) embolization were enrolled.The bilateral internal iliac artery catheterization were performed by 3D-DSA,then the images were reconstructed in every 5 degree interval to choose the optimum range of viewing angle.The origination and the degree of the origin artery and UA were calculated.The distance between the origin of UA and superior glutea artery which was identified as the locating point was measured.Results Bilateral and contralateral oblique position of ＞30°-60°were the optimal projection positions of UA.Totally 64.44％ (116/180) of UA originated from the anterior trunk of internal iliac artery,18.33％ (33/180) originated from the inferior gluteal trunk,9.44 ％ (17 / 180) originated from the internal pundenal artery,5.56 ％ (10 / 180) originated from internal iliac artery,and 2.22％ (4/180) originated from the superior gluteal artery;10.56％ (19/180) of the angle of the origin artery and UA were 0-30°,38.89％ (70/180) were ＞30°-60°,41.11％ (74/180) were ＞60°-90°,4.44％ (8/180) were＞90°-120°,2.78％ (5/180) were＞120°-150°,2.22％ (4/180) were＞150°-180°.Distance between the origin of UA and superior gluteal artery was 3.04-18.31 mm,average was (11.71±4.28)mm.Conclusion 3D-DSA can clearly display the origination,viewing angle and the distance away from superior gluteal artery.

Objective: To analyze the association between dietary inflammatory index (DII) and gallstone disease among middle-aged and elderly population, so as to provide the evidence for the prevention and control of gallstone disease. Methods: Baseline survey data were collected from the Shanghai Women's Health Study (SWHS) and Shanghai Men's Health Study (SMHS), including demographic information, gallstone disease prevalence and dietary habits. DII was calculated using 29 kinds of food parameters associated with common inflammatory biomarkers and food intake data of residents. A multivariable logistic regression model was used to analyze the association between dietary inflammatory index and gallstone disease. Results: A total of 132 312 individuals were included in the analysis. There were 59 627 males and 72 685 females. Among males, the median age was 53.07 (interquartile range, 9.73) years, 41 544 cases (69.67%) had an educational level of middle school, 4 463 cases (7.48%) had gallstone disease, and DII was -6.46 to 5.59. Among females, the median age was 50.27 (interquartile range, 9.05) years, 47 380 cases (65.19%) had an educational level of middle school, 8 090 cases (11.13%) had gallstone disease, and DII was -6.44 to 4.93. Multivariable logistic regression analysis showed that after adjusting for age, educational level, income level, smoking, alcohol consumption, tea consumption, physical activity and menopausal status (only for females), DII (OR=1.095, 95%CI: 1.002-1.196) was associated with an increased risk of gallston disease among males, but no statistically association was found among females (P>0.05). Conclusion DII might be associated with an increased risk of gallstone disease among middle-aged and elderly population.

Objective:To explore the effect of peer support system on social anxiety and resilience among new graduate nurses. Methods:Totally 36 new graduate nurses entry in 2014 who's Interaction Anxiousness Scale (IAS) score were ＞43 were selected as control group. Totally 39 new graduate nurses entry in 2015 who's IAS score were ＞ 43 were as experimental group. The control group accepted routine standardized training. The experimental group received peer support, which consisted on conduct group intervention and twining intervention (6 months). IAS and Connor-Davidson Resilience Scale(CD-RISC) were used to investigate the nurses'social anxiety and resilienceat 3 months and 6 months after intervention. The scores were analyzed by using repeated ANVOA Results: Repeated measures analysis of variance of IAS showed that, there were significant differences on the IAS scores for interaction between measure time and group processing (P ＜0.001). The differences between the two groups in the main effects of interaction and time on the total score of CD-Rescan its three dimensions score were all statistically significant (Ps ＜ 0.001). Separate analyses showed that at baseline, there was no significant difference between two groups in all variables (Ps ＞0.05). After 3 months and 6 months of intervention, the IAS scores were lower in the intervention group than in the control group (Ps ＜0.001), the CD-RISC total scores and the three dimensions scores were higher in the intervention group than in the control group (Ps ＜0.05). Conclusion: It suggests that the peer support system could improve the social anxiety and resilience of new graduate nurses.

Objective:To study combined adjuvant transcatheter arterial chemoembolization (TACE) with anti-tumor drug treatment on early hepatocellular carcinoma (HCC) recurrence in patients with microvascular invasion (MVI) after partial hepatectomy with curative intent.Methods:The clinical and pathological data of 169 patients with HCC who underwent partial hepatectomy with curative intent from January 2015 to December 2018 at the Affiliated Hospital of Qingdao University were retrospectively analyzed. MVI was diagnosed by postoperative histopathology. There were 147 males and 22 females, with the median age 56 years(ranged 32-79 years). The patients were divided into surgery group ( n=62, patients who did not receive adjuvant therapy), TACE group ( n=42, patients who only received TACE) and combined group ( n=65, patients who received TACE with anti-tumor drug) according to the therapies after resection. Patients in each group were further divided into grade M1 (mild) and grade M2 (severe) subgroups according to the severity of MVI. All patients were followed-up for observing tumor recurrence. The relapse-free survival in the three groups were compared using the Kaplan-Meier method and the log-rank test was used to compare the tumor-free survival rates. Results:The tumor-free survival rates of 169 patients at 1 and 2 years after operation were 59.2% and 40.8%. The tumor-free survival rates at 1 and 2 years after operation were 45.2% and 25.8% in surgery group, 61.9% and 40.5% in TACE group, 70.8% and 52.3% in combined group respectively. The differences among the three groups were significant: TACE group was better than surgery group, and combined group was better than TACE group, combined group was better than surgery group (all P<0.05). In TACE group and combined group, tumor-free survival rates of M1patients better than M2 patients, and the difference was significant ( P<0.05). Among M1 patients and M2 patients, tumor-free survival rates of combined group patients were better than surgery group and TACE group, the difference was significant (all P<0.05). The cumulative tumor-free survival rate was not significantly affected by different antineoplastic agents. Conclusion:Adjuvant TACE reduced the early recurrence rate of HCC patients with MVI. Adjuvant TACE combined with anti-tumor drug further reduced early tumor recurrence.

OBJECTIVETo identify the pathogenic mutation in a Chinese family with Alport syndrome.

METHODSBlood samples were collected from the members of the family. Direct DNA sequence analysis of the entire coding region and exon-intron boundaries of the COL4A5 gene was performed, and restriction fragment length polymorphism (RFLP) analysis was used to confirm the sequencing results and to test the mutation in all the family members and 200 controls.

RESULTSA novel splicing mutation of c.1517-1G to T in the COL4A5 gene was identified in all patients in the family. RFLP analysis did not detect this mutation in all the unaffected family members and the 200 controls.

CONCLUSIONThis data revealed a novel splicing mutation of c.1517-1G to T in the COL4A5 gene causing Alport syndrome in a Chinese family. Author's study enriched the spectrum of COL4A5 mutation associated with Alport syndrome.

Adult ; Asian Continental Ancestry Group ; genetics ; Base Sequence ; Case-Control Studies ; Chromosomes, Human, X ; genetics ; Collagen Type IV ; genetics ; DNA Mutational Analysis ; Female ; Genetic Linkage ; Humans ; Male ; Mutation ; Nephritis, Hereditary ; genetics ; Pedigree ; Polymorphism, Restriction Fragment Length ; RNA Splicing ; genetics