Objective To evaluate the intraoperative condition and prognosis of extracorporeal memberane oxygenation (ECMO) in patients with idiopathic pulmonary fibrosis (IPF) undergoing single lung transplantation.Methods Fifty-five patients (49 males and 6 females,aged 18-75 years,ASA physical status Ⅲ or Ⅳ) undergoing single lung transplantation were divided into ECMO group (E group,n =41) and control group (C group,n =15).Lung ischemia time,operative time,intraoperative crystal fluid volume,intraoperative colloids fluid volume,total fluid volume,albumin volume,bleeding volume,blood transfusion volume and urine volume were recorded.ECMO time,extubation time,the length of ICU stay and postoperative hospital stay were assessed.Results There was no significant difference in lung ischemia time,operative time,intraoperative crystal fluid volume,intraoperative colloids fluid volume,total fluid volume,albumin volume,bleeding volume,volume of blood transfusion,urine volume,ECMO time,extubation time,the length of ICU stay,postoperative hospital stay and mortality rate during hospitalization between the two groups.Blood plasma volume was significantly more in E group (9.3 ± 4.5 mL/kg) than that in C group (5.4 ± 4.2 mL/kg;P＜0.01).ECMO time was positively related to extubation time,the length of ICU stay and mortality rate during hospitalization (P＜0.01).Conclusion ECMO does not affect intraoperative condition and prognosis of patients.According to ECMO time we can judge the prognosis of patients.

Objective To investigate the effect of Salvianolic Acid B(Sal B)on vascular function of db/db mice and reveal the potential mechanism. Methods 20 male db/db mice were divided into 2 groups,the con-trol group(n=10)and Sal B group(n=10). 10 age-matched male C57BL/KsJ mice were used as the wild type control. Mice in Sal B group were given Sal B ,100 mg/(kg · d)by tube. Mice in db/db control group and in wild type control were given the same volume of saline. Body weight,tail blood pressure,heart rate and fasting blood glucose level were measured every week. After 6-weeks treatment ,thoracic aorta was obtained and used to detect the levels ofsuperoxide anion and NO,vascular function,eNOS,p-eNOS,AMPK and p-AMPK. Results Sal B could reduce the body weight and fasting blood glucose level of db/db mice ,but had no effect on blood pressure. Sal B could decrease the level of superoxide inon,increased NO level,and improved endothelium-dependent but not endothelium- independent diastolic function. Sal B could increase phosphorylation levels of eNOS and AMPK. Conclusion Sal B can reduce the oxidative stress ,increases NO level in vasculature ,and improves the endo-thelium-dependent vasodilation in the diabetic mice ,which may be associated with the promotion of AMPK phos-phorylation.