Purpose:To evaluate the efficacy, toxicity and side effects of the combination of gemcitabine and cisplatin in the treatment of patients with non small cell lung cancer(NSCLC).Methods:35 patients with NSCLC diagnosed by pathology or cytology were enrolled into the study. The patients received gemcitabine 1 000 mg/m 2 on d 1,8 and 15 and cisplatin 80 mg/m 2 on d 1 of 28 day cycle (4 week regiment), or received gemcitabine 1 200 mg/m 2 on d 1,8 and cisplain 80 mg/m 2 on d 8 of the 21 day cycle (3 week regiment). Results:28 of all the cases could be evaluated. The total response rate was 53.6% (all of them were partial response). Response rate of 4 week regiment and 3 week regiment were 58.3% and 50.0%, there was no significant difference between the two groups. The major toxicity and side effects included leucopenia and thromasthenia, but they were acceptable. Conclusions:The combination of gemcitabine and cisplatin is an effective and tolerable regiment in the treatment of NSCLC. Further study on the efficacy, toxicity and side effects in the treatment of NSCLC with different regimens should be carried out in the future.

AIM: To examine the expression of CD69 on NK cells at the fetomaternal interface in CBA/J?DBA/2 mice as a model of recurrent spontaneous abortion (RSA), and to evaluate the effects of lymphocyte immunotherapy (LIT) on the level of CD69 expression and the relationship with the outcomes of murine fetuses and pups. METHODS: The outcomes of murine fetuses and pups were evaluated in breeding pairs of CBA/J?DBA/2, C57BL/6?DBA/2 and BALB/c?DBA/2 mice. Both preweaning growth curves and Kaplan-Meier survival graphs of pups were constructed throughout postnatal days 1 to 21. In addition, the level of CD69 expression on NK cells at the fetomaternal interface with and without LIT were determined by two-color flow cytometric analysis, stained with PE-CD69 and FITC-DX5. The subpopulation of CD16/CD32 + NK cells was also evaluated. RESULTS: Statistically significant differences were observed between CBA/J?DBA/2 mice and normal fertile controls in the median increase of maternal weight during pregnancy, the number of pups born per litter, the median neonatal weight on postnatal day 1, and the resorption rate of fetuses. The proportion of CD69 +DX5 + cells which represents activated NK cells was significantly higher in CBA/J?DBA/2 mice compared with normal fertile controls, while efficient LIT was able to dramatically decrease the expression of CD69 on NK cells at the fetomaternal interface and this was associated with the decrease of resorption rate accordingly. CONCLUSION: The fraction of CD69 +DX5 + cells seems to be functionally important in the mechanisms by which the embryos were rejected, whereas efficient LIT is capable of reducing the abortion rate via decreasing the expression of CD69 molecules on NK cells at the fetomaternal interface.

OBJECTIVETo assess whether the plasminogen activator inhibitor-1 (PAI-1) gene 4G/5G polymorphism is associated with coronary heart disease (CHD) in Chinese patients.

METHODSPAI-1 gene 4G /5G polymorphism was analyzed in normal group (121 individuals) and CHD group (126 cases) by a combination of polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).

RESULTSThe 4G allele and 4G/4G genotype frequencies of PAI-1 gene (0.60 and 0.397) for CHD patients were higher than those (0.48 and 0.190) for healthy controls(chi-square=7.63 P<0.01; chi-square=12.67, P<0.01). The odds ratios(OR) for CHD in subjects with the 5G/5G (and 4G/5G) genotypes were 2.54 (95% CI 1.22-5.27, P<0.05) and 1.28(95% CI 1.45-2.38, P>0.05), respectively.

CONCLUSIONThese results suggest that the PAI-1 4G/4G genotype is associated with an increased risk for CHD in Chinese patients. The subjects with the 4G/4G genotype had a higher prevalence of CHD, compared to those with the 5G/5G PAI-1 genotype.

Adult ; Aged ; Aged, 80 and over ; Asian Continental Ancestry Group ; genetics ; Coronary Disease ; genetics ; Female ; Gene Frequency ; Humans ; Male ; Middle Aged ; Plasminogen Activator Inhibitor 1 ; genetics ; Polymorphism, Genetic

Objective To evaluate the application of high-throughput shell vial assay in a clinical laboratory for detection of respiratory viruses from patients with ILI in Guangzhou between January and June, 2009. Methods Six hundred and fifty-two pharyngeal swab specimens were taken from ILI patents. Centrifugation-enhanced shell vials including 4 cell lines (MDCK, Hep-2, LLC-MK2 and MRC-5) were used for culture of respiratory viruses for 2-3 days. The cultures were identified by observation of cytopathic effect (CPE) , hemmaglution or hemmadsorption test as well as immunofluorescence staining. Results A total of 161 swab samples (24.69% ,161/652) were shown to have any one of the 5 common respiratory viruses including influenza A viruses ( 38. 51% , 62/161 ), influenza B virus ( 54. 65% , 88/161 ), parainfluenza viruses (4. 96% , 8/161 ) , adenovirus ( 1. 24% , 2/161 ), and respiratory syncytial virus (0. 62% ,1/161). The turnaround time was 2d for influenza viruses, 3d for adenovirus and parainfluenza viruses respectively. Conclusions (1) The shell vial method was effective, rapid and high throughout for the detection of respiratory viruses in clinical laboratories.(2)Influenza viruses were dominant in the swab samples from patients with ILI in Guangzhou between January and June with the highest appearance in the summer influenza B vires was the most common pathogen in patients with ILI in this study.

Objective To investigate the diagnostic value of susceptibility-weighted imaging (SWI)in the application of mild traumatic brain injury.Methods The clinical data of 78 patients with mild traumatic brain injury were analyzed retrospectively.All patients received SWI and conventional MRI examination (T1WI, T2WI and FLAIR), and the number of intracerebral hemorrhagic lesions was compared between SWI and conventional MRI.Results The number of intracerebral hemorrhagic lesions of SWI examination was significantly much than that of conventional MRI examination: 39.74% (31/78)vs.21.79%(17/78),and there was statistical difference(χ2=5.898,P=0.015).SWI depicted 156 hemorrhagic lesions,and conventional MRI(T1WI,T2WI and FLAIR)depicted 16,26 and 37 hemorrhagic lesions.SWI depicted a significantly higher number of hemorrhagic lesions than conventional MRI,and there was statistical difference(Z=-4.563,-4.476 and-4.478;P<0.01).The Glasgow coma score on admission in patients with hemorrhagic lesions of SWI examination was significantly lower than that in patients without hemorrhagic lesions of SWI examination:(14.23 ± 0.80)scores vs.(14.84 ± 0.48)scores, and there was statistical difference (Z =- 3.956, P<0.01).The number of intracerebral hemorrhagic lesions in SWI examination was negative correlation with Glasgow coma score on admission(rs=-0.471, P = 0.008).Conclusions SWI has higher sensitivity in detecting intracerebral hemorrhages compared with conventional MRI.It can objectively and accurately evaluate the severity of patients with mild traumatic brain injury.

Objective To analyze the changing antibiotic resistance profile of the Klebsiella pneumoniae strains isolated from blood cultures during the last 9 years in our hospital for rational use of antibiotics. Methods Antibiotic resistance of the K. pneumoniae strains isolated from blood cultures from 2009 to 2017 was retrospectively analyzed. Medical records of the corresponding inpatients were reviewed and analyzed. Results A total of 6 917 bacterial strains were isolated from blood cultures between 2009 and 2017, of which 311 strains (4.5％) were Klebsiella pneumoniae. Complete antimicrobial susceptibility data were available for 298 of the 311 strains. The K. pneumoniae strains isolated from blood cultures showed increasing resistance rate to the antimicrobial agents in the nine-year period. The resistance rates to piperacillin-tazobactam, cefotetan, amikacin, carbapenem antibiotics increased from 0 to 48.6％. The prevalence of CRKP increased from 0 in 2009 to 48.7％ in 2017. The inpatients with K. pneumoniae isolate from blood cultures were mainly found in general surgery, cardio-thoracic surgery department, emergency room, and pediatrics surgery department. Significant difference was found between CRKP-associated inpatients and non-CRKPassociated inpatients in the time to detection of K. pneumoniae, exposure to antimicrobial agents, invasive procedure, length of hospital stay, and prognosis (P＜0.001). Conclusions The antibiotic resistance profile of the K. pneumoniae strains isolated from blood cultures showed a rising tendency during the period from 2009 to 2017. Clinical microbiology laboratory should pay more attention to strengthening antibiotic resistance surveillance. Clinicians should reduce the use of invasive procedures and use appropriate antimicrobial agents to prevent the emergence of antibiotic-resistant strains.