Pyroptosis is a new way of programmed cell death,a number of researches have found that it is associated with a variety of diseases.Inflammasome and caspase protein family play key roles in regulating pyroptosis.Intracellular pattern recognition receptor oligomers under external stimulus,then assemble with apop-totic speck-like protein containing a caspase recruitment domain and caspase precursor into inflammatory com-plex body,thus activation of caspase can induce pyroptosis.While as the upstream regulation mechanism of pyroptosis,inflammasome might be double edged swordfor tumor growth.On the one hand,inflammasome can inhibit the proliferation of tumor cells by inducing pyroptosis;on the other hand,the cumulative effect of the inflammsome can also form a suitable microenvironment for tumor cells and promote tumor growth.

[Objective] To establish n HPLC method for analysis of ginsenoside Rb1 in AITONG capsules. [Method]The HPLC system consists of G1314A variable wavelength un-visible detector,Hypersil ODS(4.6mm?250mm,5?m),HP chemstation, Hp1100 Series Quaternary Pump and Vacuum degasser (G1322A). The mobile phase was: Acetonitrile:Water (30∶70,v/v). The flowrate was 1.0ml?min-1,the injection volume was 20?l,and the detector wavelength was 203nm. [Results] The method was linear at the ginsenoside Rb1 ranging from 0.04?g to 2?g. The recovery of method was 98.26%(n=5),the average RSD was 1.41% .

Background:Disruption of intestinal epithelial tight junction and the followed barrier function play important roles in the pathogenesis of intestinal disorders. Curcumin could provide protection for the impaired barrier function. Aims:To investigate the protective effect of curcumin on ethanol-induced intestinal mucosal barrier disruption. Methods:Caco-2 cells were cultured to establish intestinal epithelial cell barrier model in vitro,and then were divided into control group, ethanol group and different concentrations of curcumin groups(5,20,80 μmol/ L curcumin). Trans-epithelial electrical resistance(TEER)and flux of sodium fluorescein for Caco-2 cell monolayers were measured to examine intestinal epithelial barrier function. Expression and localization of Occludin protein were measured by Western blotting and immunofluorescence,respectively. Cell structure was observed by transmission electron microscopy( TEM). Results:Compared with control group,TEER was significantly decreased and flux of sodium fluorescein was significantly increased (P < 0. 05),expression of Occludin protein was significantly decreased(P < 0. 05)in ethanol group. Immunofluorescence showed that Occludin protein expression was discontinuous and fluorescence intensity was low. TEM showed that brusher border was disorganized,and cell-cell junction was vague. When pretreated with curcumin,the above-mentioned indices were significantly improved,especially in 20 μmol/ L curcumin group( P < 0. 05). Conclusions:Curcumin protects ethanol-induced intestinal epithelial cell barrier disruption.

OBJECTIVE To explore the nursing management to control hospital infection in center of the blood purification,and to improve the nursing quality.METHODS All of the nursing management measure,education and strictly administered disinfection and isolation measure were established to control the hospital infection.RESULTS After the nursing management to control hospital infection was standardized and regularted and the standardized sterilization and isolation were set up,the rate of hospital infection was decreased.CONCLUSIONS Nursing management can play a main and key role in the control of hospital infection in center of the blood purification.

Objective To investigate ventricular remodeling and glucose transporter 4(GLUT4) expression in rat with type 2 diabetes mellitus(DM) complicated with myocardial infarction (MI), as well as the effects of ramipril on ventricular remodeling and CLUT4 expression. Method Diabetic animal model (DM) was produced by cholesterol-diet and intraperitoneal injedtion of streptozotocin (STZ,30 mg/kg). The successful model was identified based on the blood gulcose value > 6.7 mmol/L. Left anterior descending coronary artery was ligated to produce the model of MI. 46 wistar rats were randomly(random number) divided into 5 groups: DM + MI + placebo group(placebo group, n = 9), DM + MI + ramipril group(ramipril group, n = 10), DM + sham-operation group (DM group, n = 10),non-DM group + MI (MI group, n = 9), and non-DM + sham-operation group (n = 8). After six weeks, echocardiographic studies was performed ; Left ventricular mass index (LVMI), myocardial infarct size and myocardial collagen volume fraction(CVF) of non-infarction area were also detected. GLUT4 mRNA in the myocardium away from infarction region was measured with real-time fluorescent quantitative RT-PCR and the expression of GLUT4 protein was measured with western blot. Analysis of variance was used to compare the groups. P -values< 0.05 were considered to indicate statistically significant differences. Results Compared with Ml group and DM group,EF[(32 ±3)% vs. (65 ±4)%, P <0.01 and (32 ±3)% vs. (38 ±4)%, P <0.05 respectively] significantly decreased,while E/A ratio (P < 0.01), LVMI (P < 0.05) and CVF (P < 0.05) obviously increased in placebo group. The expression of GLUT4 mRNA and protein were similar between placebo group and DM group, however, much lower than those in MI group(P<0.01).In rampiril group, E/A ratio (P < 0.01), LVMI(3.1±0.2 vs.4.2±0.4, P <0.01) and CVF(P < 0.05) significantly decreased compared with placebo group, while EF[(45±3)% vs.(32±3)%, P <0.01] and the expression of GLUT4 mRNA and protein (P < 0.05) increased. Conclusions Rampiril could improve left ventricular hypertrophy and systolic/di-astolic dysfunction, increase the expression of GLUT4 mRNA and protein in diabetic rats complicated with MI.

Objective To investigate the successful rate and the therapeutic effect of loosening posterior tubercle of cervical transverse process with CT-guided insertion or blind insertion of small needle knife for cervical spondylotic radiculopathy. Methods Ninety patients with cervical spondylotic radiculopathy were divided into CT-guided insertion group and blind insertion group in the admission order using the random number table method, 45 cases in each group. CT-guided insertion group adopted the insertion of small needle knife following by the guide of CT, and the blind insertion group adopted the insertion of small needle knife following by cervical bony landmarks and muscles. After insertion, the position of the small needle knife in patients of the two groups was located by CT scan. If the needle knife did not arrive to the foci, it will be inserted again following by the guide of CT till successful insertion and location. The successful rate for the two times of insertion was calculated, and the pain scores were evaluated by visual analogue scale(VAS). Results(1) The successful rate for the first insertion of CT-guided insertion group was 73.3％, and that of the blind insertion group was 47.8％, the difference being significant(P < 0.01). The successful rate for the second insertion of CT-guided insertion group was 92.2％, and that of the blind insertion group was 80.0％, the difference being significant (P<0.01). (2) After treatment for one week, pain VAS scores of the two group were decreased (P < 0.01 compared with those before treatment) , and the decrease in the CT-guided insertion group was more obvious than the blind insertion group (P < 0.05). Conclusion CT-guided insertion makes the small needle knife therapy be more accurate and safer, ensures much better therapeutic effect, and brings less pain in the patients.

Objective To compare the efficacy and resistance between S?1 combined with radiotherapy and S?1 alone in the treatment of elderly patients with locally advanced gastric cancer. Methods Fifty?eight elderly patients with unresectable locally advanced ( stage Ⅲ) gastric cancer were randomly and equally divided into S?1 combined with concurrent radiotherapy group ( experimental group ) and S?1 alone group ( control group ) . The experimental group received 4 cycles of S?1 treatment with each cycle containing two?week oral administration of S?1 at a dose of 40 mg/m2 twice a day followed by one?week drug withdrawal. Gastric intensity?modulated radiotherapy was performed concurrently with a dose of 45 Gy ( 1. 8 Gy per fraction) . The control group received the same dose of S?1 alone. Short?term outcomes and adverse reactions were evaluated in the two groups. Comparison was made by chi?square test. Results All patients completed the planning treatment. The experimental group had significantly higher objective response, disease control, and symptom remission rates than the control group ( 52% vs. 24%, P=0. 03;76% vs. 45%, P=0. 016;86% vs. 48%, P=0. 005) . There were no significant differences in the incidence of nausea and vomiting, anorexia, leukopenia, diarrhea, or thrombocytopenia between the two groups ( all P>0. 05) . Conclusions S?1 treatment combined with concurrent radiotherapy improves the short?term outcomes and causes tolerable toxicities in the treatment of elderly patients with locally advanced gastric cancer.

Objective To study the diagnostic value of difusion weighted imaging in brain ischemic stroke and to summarize the MR appearances and characteristics of the false negative and false positive cases.Methods 225 cases which were highly suspected of brain ischemic stroke were studied prospectively.The final diagnosis of the cases were verified by following up examination or pathology.The diagnostic value were calculated.Results The sensitivity and specificity for the diagnosis of ischemic stroke were 93.55% and 100% at superacute stage(

Objective To better understand the age related changes of cardiac structure and function and their relationship with gender, body weight and blood pressure. Methods M mode, 2 dimensional, and Doppler echocardiographic studies were performed on 306 healthy intellectuals, including 165 males and 141 females, ranging in age from 30 to 85 years. Results Parameters in both male and female including the ratio of peak E wave to peak A wave velocity(E/A), the ratio of the right ventricular peak E wave to peak A wave velocity(E/Ar), the amplitude of aortic wall (Aao) and the angle between septum and the root of aorta (?) were all decreased with the aging significantly( P

AIM: To investigate the effect of histamine and hypoxia on the expression of eNOS mRNA and protein in cultured porcine pulmonary artery and aorta endothelial cells. METHODS: Semi-quantitative RT-PCR and immuno-cytochemistry were used. RESULTS: (1) Histamine increased eNOS mRNA expression in a dose-and time dependent manner. For pulmonary endothelial cells, the effect reached peak when exposed to 10 -5 mol/L histamine in 24 h. eNOS mRNA level was increased to 178 2%?7 7% ( P