Objective Colorectal polyp is a precancerous lesion of colorectal cancer .Aim of the study was to explore the risk fac-tors of colorectal polyp malignant transformation .Methods The related information of 75 084 colonoscopies performed from 2003 to 2012 in Southwest Hospital in Chongqing were collected and the relationship between polyp malignant transformation and the pa-tient age ,sex ,polyp location ,size or histological types was analyzed .Results From 2003 to 2012 ,polyps were diagnosed in 14 806 cases of the total 75 084 patients with a 19 .72% detection rate .There were significant difference of the left-side and right-side pol-yp detection rate in different age groups ,and the frequency of polyps distributed in the whole colorectum increased with the increase of age .The rates of epithelial neoplasia and malignant transformation increased with age .At the same time ,malignant transforma-tion rate was significant higher in polyps located in left-side than that in right-side (P<0 .0167) ,in adenoma than that in inflamma-tory hyperplastic polyp (P<0 .01) .The larger diameter and the more villus ,the higher rate of malignant transformation .Conclusion Patient age ,polyp size ,location and histological type could be considered as the significant predictors of colorectal polyp malignant transformation .It may be useful to treat the polyp with endoscopy in patient with age more than 45 and adenoma whose diameter was not less than 1 cm ,located in left-side for prevention of colorectal cancer .

Objective:To investigate the regulation of proliferation and apoptosis of oral squamous cell carcinoma(OSCC)cells by circular RNA hsa_circ_0003221(circPTK2)targeting microrNA(miR)-139-3p/IGF2BP3 axis.Methods:The mRNA expression of circPTK2,miR-139-3p and IGF2BP3 in tissues and cell lines was detected by qRT-PCR.OSCC CAL-27 cells were divided into 5 groups:sh circPTK2,sh NC,sh circPTK2+miR-139-3p inhibitor,sh circPTK2+inhibitor NC and blank control groups.Cell prolifera-tion rate,apoptosis rate,expression of related proteins and IGF2BP3 were respectively detected,and the targeting relationship between miR-139-3p and circPTK2 and IGF2BP3 was respectively verified.Results:The expression of miR-139-3p was decreased in OSCC tissues and the OSCC cell lines,and the mRNA expression of circPTK2 and IGF2BP3 was increased(P＜0.05).Silencing circPTK2 inhibited cell proliferation,decreased mRNA and protein expression of circPTK2 and IGF2BP3,and promoted cell apoptosis and miR-139-3p expression(P＜0.05).miR-139-3p inhibitor reversed the inhibitory effect of silencing circPTK2 on the malignant behavior of CAL-27 cells(P＜0.05).miR-139-3p has a targeting relationship with circPTK2 and IGF2BP3,respectively.Conclusion:Silencing circPTK2 can regulate OSCC cell proliferation and apoptosis through miR-139-3p/IGF2BP3 axis.

A small proportion of mononuclear diploid cardiomyocytes (MNDCMs), with regeneration potential, could persist in adult mammalian heart. However, the heterogeneity of MNDCMs and changes during development remains to be illuminated. To this end, 12 645 cardiac cells were generated from embryonic day 17.5 and postnatal days 2 and 8 mice by single-cell RNA sequencing. Three cardiac developmental paths were identified: two switching to cardiomyocytes (CM) maturation with close CM-fibroblast (FB) communications and one maintaining MNDCM status with least CM-FB communications. Proliferative MNDCMs having interactions with macrophages and non-proliferative MNDCMs (non-pMNDCMs) with minimal cell-cell communications were identified in the third path. The non-pMNDCMs possessed distinct properties: the lowest mitochondrial metabolisms, the highest glycolysis, and high expression of Myl4 and Tnni1. Single-nucleus RNA sequencing and immunohistochemical staining further proved that the Myl4⁺Tnni1⁺ MNDCMs persisted in embryonic and adult hearts. These MNDCMs were mapped to the heart by integrating the spatial and single-cell transcriptomic data. In conclusion, a novel non-pMNDCM subpopulation with minimal cell-cell communications was unveiled, highlighting the importance of microenvironment contribution to CM fate during maturation. These findings could improve the understanding of MNDCM heterogeneity and cardiac development, thus providing new clues for approaches to effective cardiac regeneration.
Animals ; Mice ; Diploidy ; Heart ; Myocytes, Cardiac/metabolism* ; Cell Communication ; Gene Expression Profiling ; Mitochondria ; Regeneration ; Mammals/genetics*