Objective:To study the clinical value of peripheral blood Runx3 gene CpG island's methylation in the evaluation of colon cancer's disease condition and prognosis.Methods:Methy1ation specific PCR was used to detect the Runx3 gene CpG island's methylation by Peripheral blood in colon cancer patient (observation group) and healthy people (control group).The Runx3 gene CpG island's methylation rates in different clinicopathological factors were compared.The 3 year survival rate and total survival time between patient with Runx3 gene CpG island's methylation and unmethylation were Compared.Result:The Runx3 gene CpG island's methylation rate in observation group was significantly higher than in control group (P＜0.05).The Runx3 gene CpG island's methylation rates in degree of differentiation,maximum tumor diameter,lymph node metastasis,distant metastasis,TNM staging and surgical resection were significant different (P＜0.05).The 3 year survival rate and total survival time in patient with Runx3 gene CpG island's methylation were significantly higher than in patient with Runx3 gene CpG island's unmethylation.Conclusion:Runx3 gene CpG island is hypermethylated in colon cancer's peripheral blood,which has value in the evaluation of colon cancer's disease condition and prognosis.

Objective To study the occurrence of apoptosis in acute rejection of hamster-to-rat liver transplantation and the molecular mechanism. Methods On the basis of establishment of hamster-to-rat orthotopic liver transplantation, the apoptosis in xenograft were observed by histology and in situ end-lebelling of fragmented DNA;the expression of Fas-L and TGF-?1 were observed by Immunohistochemistry. Rseults In acute rejection of liver xenograft, the apoptosis of hepatocyte was detectable, meanwhile, the expression of Fas-L and TGF-?1 were found. The more severe apoptosis, the more severe acute rejection , and the more expression of Fas-L and TGF-?1 . Conclusions Apoptosis as a mechanism of cell death exists in the acute rejection of liver xenograft, and it is closely related to the expression of TGF-?1 and Fas-L.

The impact of maintenance therapy on progression?free survival and overall survival as well as quality of life of Chi?nese patients with metastatic colorectal cancer has long been under discussion. Recently, some phase III clinical trials have revealed that maintenance therapy can signiifcantly prolong the progression?free survival while maintain an acceptable safety proifle. Based on this evidence and common treatment practice in China, we now generated one Expert Consensus on Maintenance Treatment for Metastatic Colorectal Cancer in China to further specify the neces?sity of maintenance therapy, suitable candidates for such treatment, and appropriate regimens.