We had confirmed that treatment of donor mice with Ch. Violaceum endotoxin (lipopolysacchari-de,LPS)could inhibit GVHR. In this paper, its immunological mechanisms were explored. The results showed that the LPS could augment [3H] -TdR uptake by mouse spleen cells, but decrease their proliferative responses to LPS and Con A, and suppress unidirectional MLR and NK cell activity, with inhibitory rate of 64.5%, 66.1%, 51.7% and 59.3%, respectively, suggesting it had inhibitory effects on B, T lymphocytes and NK cells. In vitro experiments using mixtures of spleen cells from treated and normal mice showed there existed non-specific suppressor cell activity in LPS-and Con A-induced lymphocyte transformation but not in MLR and NK activity assays. That meant suppressor cells were not responsible for suppressive effects on T cells and NK cells which are thought to be main effector cells mediating GVHD.

In this study, the prophylactic effect of treatment of donor mice with naturally hypotoxic Ch. violaceum endotoxin on lethal GVHD induced in a murine P→F1 combination was observed. Treatment of the donors with the endotoxin inhibited GVH splenomegaly, attenuated clinical manifestations and improved survival rate and mean survival time of dead animals. In addition, treatment with the endotoxin could enhance the bone marrow cells to form CFU-S and promote the initial graftment rate of marrow allograft.

Purpose:To study the clinical characteristics and therapy of acquired fungemia in acute leukemia patients. Methods:Retrospective analysis of the clinical characteristics and therapy of 34 acquired fungemia patients from 127 acute leukemia patients was done. Results:52 fungus strains were isolated from the blood culture of 34 acquired fungemia patients. Monilia was the main pathogen (92.4%). 24 fungemia patients also had bacteremia and the percentage was (70.6%). The overall mortality rate was 55.7% .The mortality rate of fungemia and fungemia together with bacteremia were (23.5%) and 76.4% respectively. The effective rates were 70.8% for diflucan treated group, 66.7% for the liposome treated group, and 75% for the combined treatment group respectively. Conclusions:Fungus infection has been one of the main causes of death in acute leukemia patients. So timely monitoring of the fungus infection and appropriate antifungal therapy have become the principle management to reduce the incidence rate and mortality rate of fungemia in acute leukemia patients. Diflucan and liposome are still effective antifungal drugs.

Objective To investigate the relationship between the plasma levels of ET-1,TAT,and hs-CRP and slow coronary flow syndrome (SCFS),and explore effects of coronary endothelial function,coagulation function,and inflammatory reaction on blood flow of coronary artery.Methods A total of 400 cases with normal blood flow of coronary artery by coronary angiogram was randomly selected.The coronary flow patterns were determined by corrected thrombolysis in myocardial infarction frame count method (cT-FC).Among them,45 cases whose average cTFC more than 27 were assigned as SCFS group,the other 45 cases no SCFS.Plasma levels of ET-1,TAT and hs-CRPwere examined with enzyme-linked immunosorbent assay (ELISA),and were compared between two groups.Moreover,multivariate analysis evaluating predictors of SCFS was performed with regression test.Results No statistical difference was found between two groups concerning the gender,history of hypertension,diabetes mellitus,and cigarette alcohol percentage..The plasma level of HDL in SCFS group was lower than that of no SCFS [(1.22 ± 0.42) mmol/L vs (1.44±0.34) mmol/L,t =-2.731,P ＜0.01],but the plasma level of glucose in the former was higher than that of the latter [(5.68 ±0.62) mmol/L vs (5.10 ±0.84) mmol/L,t =3.727,P ＜0.01].However,Plasma levels of ET-1,TAT and hs-CRP in SCFS were higher than that of no SCFS [(94.3 ± 16.78) ng/Lvs (83.5±12.53) ng/L,t =3.051,P ＜0.01;(12.96±3.24)μg/Lvs (8.76 ±2.64)μg/L,t =5.945,P ＜ 0.01 ; (2.48 ± 0.35) μg/L vs (1.38 ± 0.46) μg/L,t =11.259,P ＜ 0.01].Furthermore,Logistic regression analysis showed that ET-1,TAT and hs-CRP were risk factors for SCFS (OR ＞ 1.22).Conclusions Due to coronary endothelial dysfunction,endothelial inflammatory reaction,and activated coagulation function,slow coronary flow of coronary artery occurs.

OBJECTIVE To investigate the antimicrobial-resistance of Staphylococcus aureus isolates which obtained from different origins.METHODS The isolates of S.aureus were derived from clinical samples by routine methods.The identification and susceptibility test of the isolates were determined by Automated Microbiology Analyzer.Disk-diffusion test(K-B method) was used for detecting meticillin-resistant S.aureus(MRSA).?2 Test was made to identify the significance of difference.RESULTS A total of 248 S.aureus strains were isolated from blood(25),medical internal catheter(27),pus(89),sputum(93),and urine(14).All isolates of S.aureus were sensitive to nitrofurantoin and vancomycin.The resistant rates of S.aureus isolates from pus to chloramphenicol,gentamicin,oxacillin,penicillin,rifampicin,tetracycline and levofloxacin all significantly lower than those of isolates from sputum(P

OBJECTIVE To investigate the antimicrobial-resistant profile of aminoglycosides-resistant Klebsiella pneumoniae isolated from Wenzhou.METHODS Between Jan 2006 and Sep 2007,non-replicate clinical isolates of K.pneumoniae were consecutively collected from inpatients in a teaching hospital in Wenzhou,China.Antibiotic susceptibility test was determined by the disc diffusion method recommended by CLSI.Sixty-four gentamicin-resistant isolates were investigated for the presence of ESBLs by the CLSI-recommended confirmatory tests.RESULTS Of the 64 gentamicin-resistant isolates,the resistant rates to ciprofloxacin,levofloxacin cefoxitin,tetracycline,trimethoprim/sulfamethoxazole,ceftazidime and cefotaxime were 7.5%,29.7%,39.1%,85.9%,76.6%,67.2%,and 73.4%,respectively.All isolates were susceptible to imipenem.73.4% of gentamicin-resistant isolates produced ESBLs.CONCLUSIONS Gentamicin-resistant K.pneumoniae is multi-resistant to antibiotics.

ystolic LS and RS are equal in the indentification of the infarcted segments.

BACKGROUND: High tibial osteotomy is a classic operation for the treatment of early simple medial compartment osteoarthritis of the knee. Three-dimensional (3D) printing can be used to make personalized surgical tools. We have used 3D printing technology to assist high tibial osteotomy for the treatment of knee osteoarthritis. OBJECTIVE: By comparing traditional methods, to analyze the advantages and disadvantages of the high tibial osteotomy assisted by personalized 3D printing osteotomy combined with arthroscopic debridement for the treatment of osteoarthritis of the knee with varus deformity. METHODS: Totally 47 cases undergoing lateral closed wedge high tibial osteotomy combined with knee joint debridement were randomly assigned to two groups. The 3D printing osteotomy group (n=21) received personalized 3D printing osteotomy. The traditional osteotomy group (n=26) received traditional osteotomy. The operation time, number of intraoperative C-arm fluoroscopy and blood loss, limb alignment on the affected side 1 month after surgery, and Hospital of Special Surgery scores on the affected side knee joints at 1, 3, 6,12 and 24 months after surgery were compared between the two groups. This study was approved by the Ethics Committee of First Hospital of Putian City. All patients signed the informed consent. RESULTS AND CONCLUSION: (1) The intraoperative 3D printing osteotomy module was consistent with the preoperative simulated place. (2) The operation time was significantly shorter in the 3D printing osteotomy group than in the traditional osteotomy group [(69. 71 ±3. 17), (92. 92±5. 91) minutes, (=-17. 21, P < 0. 01]. The number of C-arm fluoroscopy was significantly less in the 3D printing osteotomy group than in the traditional osteotomy group [(5. 71 ±1. 62), (18. 15±3. 00) times, f=-18. 12, P < 0. 01]. Blood loss was significantly less in the 3D printing osteotomy group than in the traditional osteotomy group [(275. 24±53. 82), (507. 69±45. 19) mL, f=-16. 10, P< 0. 01]. (3) There was no difference between the two groups in the limb alignment on the affected side at 1 month after operation and the Hospital of Special Surgery score on the affected knee at 24 months after operation (all P > 0. 05). (4) In conclusion, compared with traditional osteotomy, personalized 3D printing-assisted osteotomy in lateral closed wedge high tibial osteotomy can significantly shorten the operation time, reduce the number of intraoperative C-arm fluoroscopy and the amount of blood loss, but there is no significant difference in knee function between the two groups at 2 years after surgery.

Objective To investigate clinical effect of recombinant human growth hormone (rhGH)for inter-venting central precocious puberty in children,to provide reference for clinical treatment.Methods 80 patients with growth deceleration slows CCP were selected,when height growth rate below 4cm/year,40 patients on the basis of joint GnRHa used rhGH treatment were selected as the observation group,and the remaining 40 patients refused to use rhGH,continued to use of GnRHa treatment were selected as the control group.After treatment predicted height,height,bone age,growth rate and other indicators were compared.Results In observation group,the actual height Ht, predicted height PAH,the growth rate Gv,serum insulin -like growth factor -1 (IGF -1 )after treatment were (1 44.48 ±6.59)cm,(1 54.94 ±4.52)cm,(4.43 ±0.64)cm/6months,(1 32.25 ±8.84)ng/mL,compared to before treatment,the differences were statistically significant (t =6.548,P <0.01 ;t =5.734,P <0.01 ;t =28.869,P <0.01 ;t =20.65,P <0.01 ),compared with the control group,the differences were significant (t =3.943,P <0.01 ;t =4.759,P <0.01 ;t =28.247,P <0.01 ;t =20.882,P <0.01 ),there were no differences in other indices;hor-mones FSH,LH and other indicators of the two groups before and after treatment,and blood sugar,thyroid indicators showed no abnormality.Conclusion Recombinant human growth hormone in the treatment of central precocious puberty deceleration in children does not increase bone age,which will help improve the growth rate and predicted height,which worthy of clinical application.

Objective To explore the relationships between brain-derived neurotrophic factor gene G196A polymorphisms and psychopathology and the cognitive function in schizophrenic patients.Methods 224 patients and 220 normal controls were examined with polymerase chain reaction(PCR),denaturing polyacrylamide gel electrophoresis and silver staining to determine genotype and allele of G196A.Clinical symptoms were assessed with the Positive and Negative Symptom Scale (PANSS).Cognitive function was assessed with Wisconsin card sorting test (WCST) and Trail Making Test(CPT).To analyse the differences of the scores of Scale for the Assessment of Positive Symptoms(SAPS) and Scale for the Assessment of Negative Symptoms (SANS) among the patients with the genotype G/G,G/A and A/A.At the same time,the differences of the performance of WCST and CPT were analyzed.Results ①There were no significant differences of the genotypes G/G,G/A and A/A and allele G and A between patients and controls.②There were significant differences of the genotypes A/A between patients with Positive Symptoms and patients with Negative Symptoms (x2 =4.558,P ＜ 0.05).③There were no significant differences in the performances of complete categorizations and persistent wrong numbers of WCST and the performances of CPT among three groups of patients with genotype G/G,G/A and A/A (One-Way ANOVA,all P ＞ 0.05).Conclusion BDNF gene G196A polymorphisms are not associated with schizophrenia and the cognitive function but are associated with positive symptoms of schizophrenia.