1.Comparison of pharmacokinetics of remifentanil during general anesthesia in children and adults
Manhe ZHANG ; Jingui GAO ; Kaizhi XU
Chinese Journal of Anesthesiology 2011;31(2):154-156
Objective To compare the pharmacokinetics of remifentanil during general anesthesia in children and adults.Methods Eight children(4 male,4 female)and 8 adults(4 male,4 female),undergoing elective operation under general anesthesia,were randomly divided into 2 groups(n=8 each):group adults(aged 19-60 yr,weighing 45-81 kg)and group children(aged 10 months-7 yr,weighins 7.2-21.0 kg).Remifentanil 5μg/kg was injected intravenously during induction of anesthesia.Arterial blood samples 1.0 ml were taken at 1,2,3,5,7,10,15,20,25,30,45 and 60 min after injection for determination of the plasma concentrations of remifentanil.The pharmacokinetic parameters were calculated using software 3P97.Results Elimination half-life was significantly shorter and apparent volume of distribution and clearance were significantly greater in children than in adults(P<0.05),while no significant change was found in the other pharmacokinetic parameters between the two groups(P>0.05).Conclusion There is difference in the pharmacokineties of remifentanil during general anesthesia between children and adults.The plasma concentration of remifentanil is lower in children than in adults after using the same dose,and the dose should be increased appropriately.
2.Effects of sevoflurane pretreatment on myocardial injury in patients undergoing cardiac valve replacement with cardiopulmonary bypass
Chengjie GAO ; Jingui YU ; Huixia WANG ; Bo LI ; Xiaoming Lü ; Lufeng XU ; Guimao CAO ; Jishun NING ; Guanhua JIANG ; Aijun NIU ; Hailong DONG
Chinese Journal of Anesthesiology 2012;32(3):278-281
Objective To investigate the effects of sevoflurane pretreatment on the myocardial injury in patients undergoing cardiac valve replacement with cardiopulmonary bypass (CPB).Methods Twenty NYHA class Ⅱ or Ⅲ patients,aged < 60 yr,undergoing cardiac valve replacement with CPB,were randomly divided into 2 groups (n =10 each):sevoflurane group (group S) and control group (group C).The patients were premeditated with intramuscular morphine and scopolamine.Anesthesia was induced with iv injection of midazolam 0.05-0.08 mg/kg,fentanyl 10-15 μg/kg and pipecuronium 0.08-0.10 mg/kg.Anesthesia was maintained with intermittent iv boluses of midazolam,fentanyl and pipecuronium and in addition sevoflurane was inhaled before aortic clamping and the end-tidal concentration was rapidly increased to 1.0% and maintained at the level for 5 min in group S.Blood samples were taken from the central vein before skin incision (T1),immediately after aortic clamping (T2 ),at 0 and 30 min after aortic unclamping (T3-4),and at 2,6,12 and 24 h after operation (T5-8) for determination of the concentration of serum cardiac troponin Ⅰ (cTnI) and activities of creatine kinase (CK) and creatine kinase isoenzyme-MB (CK-MB).Myocardial specimens were taken from right auricle before aortic clamping and at 10 min after aortic unclamping for electron microscopic examination.Results The concentration of serum cTnI and activities of CK and CK-MB were significantly increased at T4-8 in both groups ( P < 0.05).The serum cTnI concentration at T4-8 and the activities of CK and CK-MB at T8 were significantly lower in group S than in group C ( P <0.05).Different degrees of mitochondrial swelling were observed after aortic unclamping in both groups,but the changes were milder in group S than in group C.Conclusion Sevoflurane pretreatment can attenuate the myocardial injury in patients undergoing cardiac valve replacement with CPB.
3.Survey on situation and cognition of influenza and pneumonia vaccination in patients with pneumoconiosis and pneumoconiosis comorbided with COPD in Hunan Province
Jin XUE ; Meian TANG ; Jingui XU ; Lang LIU ; Sheng PENG ; Xiang ZHU ; Xiongbin XIAO
Journal of Chinese Physician 2020;22(10):1449-1451,1456
Objective:To understand the current status and influencing factors of influenza and pneumonia vaccination in patients with pneumoconiosis and pneumoconiosis comorbided with Chronic obstructive pulmonary disease (COPD) in Hunan Province, and provide a basis of vaccination planning strategies for the patients.Methods:Inpatients pneumoconiosis and completed 1-year follow-up in the Hunan Prevention and Treatment Institute for Occupational Disease in May 2019 were selected as the research objects, and the data of their age, gender, education level, medical insurance type, number of hospitalization, smoking status, and comorbidity of COPD were collected retrospectively. They were followed up for 1-year of the vaccination status of influenza and pneumonia, and the main factors influencing vaccination decision. Unconditional logistic regression analysis was used to analyze the factors influencing vaccination.Results:A total of 474 hospitalized pneumoconiosis patients were included, of which 96 were comorbided with COPD; the follow-up results showed that the awareness rate of pneumoconiosis patients with influenza and pneumonia vaccine was 13.1%, and the vaccination rate within one year was 7.6%. The vaccination rate of pneumoconiosis patients with COPD within one year was 8.3%. The positive correlation factors of the vaccination were multiple hospitalization, residence of >3 people and comorbided with COPD. The route of vaccination is mainly by doctors' recommendation.Conclusions:The vaccination rate of influenza and pneumonia in pneumoconiosis and pneumoconiosis patients with COPD in Hunan Province is low; the main way for patients to learn is doctor education, so it is necessary to strengthen the health education of pneumo-coniosis doctors and patients to improve the vaccination rate.
4.Study on the application value of combined spinal-epidural anesthesia under the assistant of ultrasound localization on patients with lower limb fracture
Tao LIU ; Xu LI ; Hongliang QI ; Cheng WANG ; Gang LUO ; Jingui LI
China Medical Equipment 2024;21(2):94-97
Objective:To explore application value of combined spinal-epidural anesthesia under the assistant of ultrasound location on lower limb fractures.Methods:A total of 80 patients with lower limb fractures who underwent surgical treatment at the 901th Hospital of the PLA Joint Service Support Force from July 2020 to July 2022 were selected,and they were divided into a control group and an observation group according to the random number table method,with 40 cases in each group.The control group received epidural anesthesia under ultrasound,and the observation group received the combined lumbar-epidural anesthesia under ultrasound.The lidocaine dosage,anesthetic block time,anesthesia onset time and dosage of additional analgesic,as well as the changes in vital signs such as diastolic blood pressure(DBP),systolic blood pressure(SBP)and heart rate(HR),between the two groups of patients were compared.In addition,the thrombelastogram,anesthetic effects and occurrence of adverse reactions also were compared between two groups.Results:The lidocaine dosage,anesthetic block time,anesthesia onset time and dosage of additional analgesic of the observation group were significantly higher than those in the control group,and the differences were statistically significant(t=8.657,23.483,12.785,16.600,P<0.05),respectively.There were no statistically significant differences in DBP,SBP,HR,comprehensive coagulation index(CI),reaction time(R),formation time of blood cell agglutination(K)and incidence of adverse reactions between two groups before anesthesia,at the 30th minute after anesthesia and after the end of anesthesia(P>0.05).Compared with α angle before anesthesia and at the 30th minute after anesthesia,the α angles of the two groups increased significantly after anesthesia(t=6.564,5.783,P<0.05),respectively.The mechanical strength(G)of the blood clot and the maximum amplitude(MA)of chromatogram of the observation group were significantly reduced after completed anesthesia,and the differences of them were statistically significant(t=8.480,4.236,P<0.05),respectively.There were no significant differences between G and MA in the control group(P>0.05).The total effective rate of the observation group was 97.50%,which was significantly higher than 80.00% of the control group,and the difference was statistically significant(x2=4.507,P<0.05).Conclusion:The combined spinal-epidural anesthesia under the assistant of ultrasound location can effectively reduce the amount of anesthetics in patients with lower limb fracture,which has fast block and effect.In addition,it has little influence on circulatory function,and it is less likely to form a hypercoagulable state.It has better clinical anesthesia effect.
5.The R protein of SARS-CoV: analyses of structure and function based on four complete genome sequences of isolates BJ01-BJ04.
Zuyuan XU ; Haiqing ZHANG ; Xiangjun TIAN ; Jia JI ; Wei LI ; Yan LI ; Wei TIAN ; Yujun HAN ; Lili WANG ; Zizhang ZHANG ; Jing XU ; Wei WEI ; Jingui ZHU ; Haiyan SUN ; Xiaowei ZHANG ; Jun ZHOU ; Songgang LI ; Jun WANG ; Jian WANG ; Shengli BI ; Huanming YANG
Genomics, Proteomics & Bioinformatics 2003;1(2):155-165
The R (replicase) protein is the uniquely defined non-structural protein (NSP) responsible for RNA replication, mutation rate or fidelity, regulation of transcription in coronaviruses and many other ssRNA viruses. Based on our complete genome sequences of four isolates (BJ01-BJ04) of SARS-CoV from Beijing, China, we analyzed the structure and predicted functions of the R protein in comparison with 13 other isolates of SARS-CoV and 6 other coronaviruses. The entire ORF (open-reading frame) encodes for two major enzyme activities, RNA-dependent RNA polymerase (RdRp) and proteinase activities. The R polyprotein undergoes a complex proteolytic process to produce 15 function-related peptides. A hydrophobic domain (HOD) and a hydrophilic domain (HID) are newly identified within NSP1. The substitution rate of the R protein is close to the average of the SARS-CoV genome. The functional domains in all NSPs of the R protein give different phylogenetic results that suggest their different mutation rate under selective pressure. Eleven highly conserved regions in RdRp and twelve cleavage sites by 3CLP (chymotrypsin-like protein) have been identified as potential drug targets. Findings suggest that it is possible to obtain information about the phylogeny of SARS-CoV, as well as potential tools for drug design, genotyping and diagnostics of SARS.
Amino Acid Sequence
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Base Composition
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Base Sequence
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Cluster Analysis
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Computational Biology
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Conserved Sequence
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genetics
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Evolution, Molecular
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Gene Components
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Genome, Viral
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Molecular Sequence Data
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Mutation
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genetics
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Phylogeny
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Protein Structure, Tertiary
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RNA Replicase
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genetics
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SARS Virus
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genetics
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Sequence Analysis, DNA
6.Systematic Identification and Quality Evaluation of Anemonis Flaccidae Rhizoma from 16 Different Places of Origin
Xin HU ; Xu WANG ; Man LIU ; Hong PEI ; Zhi-guo ZHU ; Bi-sheng HUANG ; Yi-fei LIU ; Cheng-wu SONG ; Zhi-gang HU
Chinese Journal of Experimental Traditional Medical Formulae 2020;26(20):132-139
Objective:To construct a systematic identification system of Anemonis Flaccidae Rhizoma, and to evaluate the comprehensive quality of Anemonis Flaccidae Rhizoma from 16 regions in China, so as to lay a foundation for its origin selection and clinical medication safety. Method:The authenticity of Anemonis Flaccidae Rhizoma was quickly identified by traditional identification method and DNA barcode molecular identification technology, and HPLC-UV was used to determine the contents of 5 active ingredients in Anemonis Flaccidae Rhizoma. All high pressure chromatographic separations were performed with a Welch Ultimate XB-C18 column (4.6 mm×250 mm, 5 μm), the mobile phase consisted of acetonitrile-0.01% trifluoroacetic acid aqueous solution (30∶70) at a flow rate of 1.0 mL·min-1. The detection wavelength was set at 210 nm and the column temperature was maintained at 30 ℃. Result:The authenticity of Anemonis Flaccidae Rhizoma could be precisely and rapidly identified by ribosomal DNA internal transcribed spacer 2 (ITS2) sequence and traditional identification methods. BLAST comparative analysis found that medicinal materials from 16 areas were all
7.Complete genome sequences of the SARS-CoV: the BJ Group (Isolates BJ01-BJ04).
Shengli BI ; E'de QIN ; Zuyuan XU ; Wei LI ; Jing WANG ; Yongwu HU ; Yong LIU ; Shumin DUAN ; Jianfei HU ; Yujun HAN ; Jing XU ; Yan LI ; Yao YI ; Yongdong ZHOU ; Wei LIN ; Hong XU ; Ruan LI ; Zizhang ZHANG ; Haiyan SUN ; Jingui ZHU ; Man YU ; Baochang FAN ; Qingfa WU ; Wei LIN ; Lin TANG ; Baoan YANG ; Guoqing LI ; Wenming PENG ; Wenjie LI ; Tao JIANG ; Yajun DENG ; Bohua LIU ; Jianping SHI ; Yongqiang DENG ; Wei WEI ; Hong LIU ; Zongzhong TONG ; Feng ZHANG ; Yu ZHANG ; Cui'e WANG ; Yuquan LI ; Jia YE ; Yonghua GAN ; Jia JI ; Xiaoyu LI ; Xiangjun TIAN ; Fushuang LU ; Gang TAN ; Ruifu YANG ; Bin LIU ; Siqi LIU ; Songgang LI ; Jun WANG ; Jian WANG ; Wuchun CAO ; Jun YU ; Xiaoping DONG ; Huanming YANG
Genomics, Proteomics & Bioinformatics 2003;1(3):180-192
Beijing has been one of the epicenters attacked most severely by the SARS-CoV (severe acute respiratory syndrome-associated coronavirus) since the first patient was diagnosed in one of the city's hospitals. We now report complete genome sequences of the BJ Group, including four isolates (Isolates BJ01, BJ02, BJ03, and BJ04) of the SARS-CoV. It is remarkable that all members of the BJ Group share a common haplotype, consisting of seven loci that differentiate the group from other isolates published to date. Among 42 substitutions uniquely identified from the BJ group, 32 are non-synonymous changes at the amino acid level. Rooted phylogenetic trees, proposed on the basis of haplotypes and other sequence variations of SARS-CoV isolates from Canada, USA, Singapore, and China, gave rise to different paradigms but positioned the BJ Group, together with the newly discovered GD01 (GD-Ins29) in the same clade, followed by the H-U Group (from Hong Kong to USA) and the H-T Group (from Hong Kong to Toronto), leaving the SP Group (Singapore) more distant. This result appears to suggest a possible transmission path from Guangdong to Beijing/Hong Kong, then to other countries and regions.
Genome, Viral
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Haplotypes
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Humans
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Mutation
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Open Reading Frames
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Phylogeny
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SARS Virus
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genetics