The type of chronic diseases and number of patients with chronic disease are increasing all over the world. The adherence of health management in patients with chronic disease has directly impact on their health outcomes, quality of life and related health cost. The benefit of improvement in adherence of health management is even more than the improvement in treatment. Mobile health is a new way of health care to provide convenient, accessible and effective health management support. Additionally, mobile health care has been used in the health management of patients with chronic diseases. The aim of this study is to review the studies focus on using mobile health care technology in improving health management adherence, including the characteristics of participants, the intervention tools, the diseases have been managed and the outcome evaluation.

Objective To investigate the effects of down-regulation of lemur tyrosine kinase 3 (LMTK3) on the cytobiological behaviors of human lung cancer A549 cells.Methods The expression of LMTK3 in A549 cells was interfered with small hair RNA (shR-NA),and the expression level of LMTK3 was determined by RT-PCR.The effects of LMTK3 on the proliferation,migration,cell cycle and apoptosis of A549 cells were determined by the CCK-8 assay,scratch assay,Transwell assay and flow cytometry,respectively.Results The shLMTK3 cells with stably low expression of LMTK3 and control cells (Scramble) with normal expression of LMTK3 were successfully obtained.The relative proliferation rates of shLMTK3 cells cultured for 24,48 and 72 hours were significantly lower than those in the control cells (0.305 ±0.018 vs 0.354 ±0.011,t =5.24,P＜0.01;0.461 ±0.044 vs 0.551 ±0.027,t =3.91,P ＜0.01;0.74 ± 0.029 vs 0.881 ± 0.028,t =7.70,P ＜ 0.01).The relative migration rate of shLMTK3 cells 24 hours after scratching was significantly lower than that of control cells (0.51 ±0.096 vs 1.00 ± 0.029,t =4.81,P ＜ 0.01).Transwell assay showed that the number of migration cells in shLMTK3 group was significantly less than that in Scramble group (161 ±9.29 vs 308.66 ± 17.60,t =7.42,P ＜ 0.05).The results of flow cytometry showed that shLMTK3 cells were blocked at G1 phase (t =4.35,P ＜ 0.05),and that the inhibition of LMTK3 had no influence on the apoptosis of A549 cells.Conclusion Down-regulation of LMTK3 expression in human lung cancer A549 cells may inhibit the proliferation and migration of A549 cells significantly,indicating that the abnormal expression of LMTK3 in lung carcinoma cells may regulate the biological behaviors and progression of tumors.

Serum 25-(OH)D3concentration was determined in subjects with different types of obesity. The serum 25-(OH)D3levels in overweight or obese and central obese subjects were lower than that in non-obese subjects.(P＜0.01). In a multiple linear regression analysis serum 25-(OH)D3was independently and negatively correlated with body mass index and waist circumference.

[ ABSTRACT] AIM:To investigate the effect of Jia-jian-yi-yin decoction on endothelium-dysfunction in ovariecto-mized rats.METHODS:The ovariectomized rats were treated with Jia-jian-yi-yin decoction or turbid liquid of estradiol va-lerate for 8 weeks.The vascular ring tension was measured.Scanning electron microscopy and Western blotting were ap-plied to assess the function of endothelium-dependent dilation, aortic endothelial morphology and the expression of endothe-lial lipase.The pathologic sections were prepared to observe the effect of Jia-jian-yi-yin decoction on the uterus.RE-SULTS:In ovariectomized rats, the decrease in endothelium-dependent relaxation to acetylcholine ( ACh) was reversed to normal level, the endothelial morphology returned to normal without lipid accumulation and the endothelial lipase expression was decreased by Jia-jian-yi-yin decoction.Furthermore, no obvious change of the wet weight of uterine between the ovari-ectomized rats with or without Jia-jian-yi-yin decoction treatment was observed.CONCLUSION:Jia-jian-yi-yin decoction may have protective effects on endothelium-dependent vasodilation and aortic endothelial morphology in estrogen-deficient animals.

Objective To discuss the correlation between MSCT morphological index of emphysema and pulmonary function test (PFT) indexes. Methods We randomly selected 33 cases with chronic obstructive pulmonary disease (COPD),and conducted CT and PFT in a week. The whole lung was scanned by MSCT at the end of deep aspiration,and the post-processing workstation was used for quantitative determination of a total of 12 morphological indexes,including the density of lung, pulmonary diameter line,lung volume,and etc,then their correlation with lung function index was analyzed.Results FEV1.0%had correlation with upper right lung density ( =0.391, =0.391), lower right lung density ( =0.465, =0.038),the mean lung density ( =0.576,=0.576), and total lung capacity (=0.471, =0.471),upper right lung volume ( =0.413, =0.049) . FEV1.0/FVC had correlation with left lower lung density ( =0.392, =0.024), the mean lung density ( =0.703, = 0.000), total lung transverse diameter ( = 0.521, = 0.521), upper right lung maximum transverse diameter ( =0.627, =0.627), and total lung capacity ( =0.549, =0.549) correlation. FVC had correlation with upper right lung maximum transverse diameter ( = 0.378, = 0.378), characterized by maximum transverse diameter (=0.349, =0.037) . TLC had correlation with mean lung density (=0.523,=0.523), upper right lung maximum transverse diameter ( =0.454, =0.454), and total lung capacity (= 0.001), the right lung volume ( = 0.492, = 0.492) . Conclusion MSCT lung morphology quantitative indicators have good clinical application value in the diagnosis and assessment of emphysema patients.

Objective To evaluate the clinical efficacy and adverse effects of brucea javanica oil emulsion combined with DP chemotherapy in treating advanced non-small-cell lung cancer.Methods Totally 48 patients with advanced non-small-cell lung cancer were divided into two groups randomly by mechanical sampling method.Twenty-four cases in treatment group were treated by brucea javanica oil emulsion combined with DP chemotherapy, while 24 cases in control group were treated by DP chemotherapy only.The clinical effects were evaluated after treatment of two cycles.Results The short-term effective rate was 54.2% (13/24) in treatment group and 45.8% (11/24) in control group, and there was no significant difference between two groups ( χ2 = 0.333, P = 0.564).The rate of increased and stable life quality was 87.5%(21/24) in treatment group and 58.3%(14/24) in control group,and there was significant difference between two groups (χ2 = 5.169,P = 0.023).The rate of increased and stable weight was 79.2% (19/24) in treatment group and 45.8%( 11/24) in control group, and there was significant difference between two groups (χ2 = 5.689,P = 0.017).The incidence of nausea or vomiting was 45.8% (11/24) in treatment group and 41.7%( 10/24 ) in control group, and there was no significant difference between two groups (χ2 = 0.085, P = 0.771 ).Compared with those in control group, patients in treatment group had less adverse effects in decreasing of peripheral blood leucocytes and showed better immune function.Conclusion Brucea javanica oil emulsion combined with DP chemotherapy in treating advanced non-small-cell lung cancer has good clinical effect, especially enhances the quality of life, improves immune function and decreases the adverse effects of chemotherapy.

Objective To explore the changes of plasma level of adrenomedullin(ADM)in congenital heart disease patients with heart failure,analyze the relationship between ADM and the severity of heart failure, assess the value of plasma ADM in the diagnosis of CHF and evaluate the cardiac function in pediatric patients with congenital heart disease. Methods 46 patients with CHD were enrolled according to the modified Ross Score. They were divided into three groups:mild CHF group(n = 16)and moderate CHF group(n = 18)and severe CHF group(n = 12). 25 matched normal children were enrolled as controls. Plasma ADM was measured with radioimmunoassay(RIA)and the contents of NT-proBNP were determined among all the patients. LVEF and E/A in all of them were also detected. The relationship between plasma ADM level and modified Ross Score and echocardiographic cardiac functional indexes was analyzed. Results Plasma ADM was positively correlated with modified Ross Score(r = 0.65,P < 0.01). Plasma ADM concentration in the severe CHF group was significantly higher than that of mild and moderate CHF group(P < 0.01),and plasma ADM concentration in the mild CHF group was higher than that in the control group(P<0.05).Plasma ADM was negatively correlated with LVEF and E/A(r =-1.05,P < 0.01). Conclusion Plasma ADM level could be used to assess cardiac function and diag-nose CHF with CHD.

Objective:To explore the potential application value of photobiomodulation and drug combination therapy in the treatment of Alzheimer's disease (AD).Methods:Twenty-five APPswe/PS1dE9 double transgenic mice were randomly divided into model control group, donepezil hydrochloride (DH) group, DH+10 Hz group, DH+20 Hz group, and DH+40 Hz group ( n=5); and 5 C57BL/6J mice were selected as blank control group. Mice in the DH group, DH+10 Hz group, DH+20 Hz group, and DH+40 Hz group were given intragastric administration of DH (1.3 mg/[kg·d] for 4 weeks). The mice in the DH+10 Hz group, DH+20 Hz group, and DH+40 Hz group were irradiated with 1050 nm near-infrared light at 10 Hz, 20 Hz and 40 Hz frequencies for 6 min/d for 4 consecutive weeks. After all these treatments, Morris water maze was used to detect the behavioral cognitive ability of mice in each group; Congo red staining was used to detect the amyloid deposition in hippocampal CA1 area; and ELISA was used to detect the protein expression levels of amyloid protein (Aβ) 1-40 and Aβ 1-42 in the hippocampus. Results:(1) As compared with that in the model control group, the escape latency of mice in the DH+40 Hz group was significantly shortened since the 4 th d of navigation test, and that in the DH group was significantly shortened since the 5 th d of navigation test ( P<0.05); that in the DH+40 Hz group was obviously shortened as compared with that in the DH group without significant difference ( P>0.05). As compared with the those in the model control group, the times of crossing the primary platform of mice in the DH group and DH+40 Hz group were singifcantly increased ( P<0.05), and those in the DH+40 Hz group were similar to those in the DH group. (2) DH+40 Hz group showed scattered brick-red dotted patches, the staining depth and distribution range were obviously smaller than those in the model control group, and the nuclear structure arrangement was more orderly than that in the model control group; the staining depth and number of brick red patches in the DH+40 Hz group were smaller than those in the DH group. (3) The concentrations of Aβ 1-40 and Aβ 1-42 in the hippocampal tissue homogenate of DH+40 Hz group were significantly decreased as compared with those in the model control group ( P<0.05). As compared with those in the DH group, the concentrations of Aβ 1-40 and Aβ 1-42 in hippocampal tissue homogenate of DH+40 Hz group were decreased without significant difference ( P>0.05). Conclusion:The combined treatment strategy of 40 Hz 1050 nm near-infrared light and DH may have the potential to improve the behavioral cognition of AD mice and moderately reduce the deposition of Aβ in the brain tissues.

Objective To explore a new efficient extraction method of dorsal root ganglions(DRGs)of rats by exploring and extracting DRGs reversely along the nerve traveling pathway. Methods The DRGs were extracted by the traditional method of opening the intervertebral foramina and the new method, extracting DRGs reversely along the nerve traveling pathway,respectively. The time consuming and the number of intact DRGs obtained with these two method were compared. Results The number of intact DRGs(L3-5segments,both sides)extracted from each rat with the traditional method was(3.08 ± 1.31),and the average time consuming of each DRG was(5.58 ± 1.21)min. As for the new method ,the number of intact DRGs extracted from each rat was(4.29 ± 1.08), and the average time consuming was(1.69 ± 0.91)min,significantly better than that of the traditional method(P < 0.05 for both). Conclusions The new method of exploring and extracting DRGs reversely along the nerve traveling pathway is more efficient for obtaining intact DRGs of rats,providing more useful tissue materials for subsequent culture and morphological studies of DRG cells.

Objective:To understand mothers′ benefit finding in caring for thier children with Acute Lymphoblastic Leukemia (ALL).Methods:By purposive sampling method, eleven mothers who were mainly involved in daily care of their children with ALL were selected. Semi-structured in-depth interviews were conducted with the 11 mothers. The data were analyzed by content analysis method.Results:Mothers had benefit finding which could be mainly classified into three themes: individual growth, family relationship enhancement, and multi-source society support.Conclusions:Mothers of children with ALL have experienced a sense of benefit finding in the process of caring for their children. Nurses are suggested to help mothers in exploring benefit finding, actively guide and effectively utilize the sense of benefit finding in mothers of children with ALL, so as to alleviate mothers′ adverse psychological state and to promote mothers′ better coordination with follow-up treatment and caring for the children.