Objective To evaluate the expression of macrophage migration inhibitor factor (MiF) and cyclinD1 in pancreatic carcinoma and their relationships with clinical pathology characteristics. Methods The expression of MIF and eyclinD1 in 89 carcinoma and 5 normal pancreatic tissues was detected with immunohis-tochemistry methods, and the relationships among MIF and cyclinD1 expression and clinicopathological factors were studied. Results The overexpression of MIF and cyclinD1 was found in 88.8%, and 50. 6% of pancre-atic carcinoma tissues respectively. The overexpression of MIF had a significant correlation with Ⅰ,Ⅱ,Ⅲ,Ⅳ tumor stage (69. 2%, 94. 7%, 96. 4%, 100%, P <0.05), while the positive expression rate of cyclinD1 only had a significant correlation with tumor stages Ⅲ,Ⅳ (33. 3%, 68. 8%, P <0. 05). Both of the two proteins had a correlative tendency with pathological grade and lymph node metastasis. The different expression of MIF between pancreatic carcinoma with and without liver metastasis had no statistical significance, (100% ,85.9%, P >0. 05)while there was a statistically significant difference about cyclinD1 (66. 7% ,46. 5% ,P <0. 05). A significant positive correlation was also found between MIF and cyclinD1 (P < 0. 05). Conclusion The ex-pression of MIF and CyclinD1 was higher in pancreatic cancer tissues than in normal tissue, and they may be associated with the malignant stage, tumor differentiation, local lymph node and liver metastasis of this tumor.

Objective To study the influence of long-term home noninvasive positive pressure ventilation (HNPPV) on respiratory muscle strength in patients with stable severe chronic obstructive pulmonary disease (COPD).Methods Sixty-four patients with stable severe COPD discharged from Huabei Oil-field Hospital,Renqiu,Hebei were divided into two groups,one (n=24) with HNPPV plus conventional therapy,and the other (n=40) with conventional therapy plus long-term oxygen therapy as controls.All parameters were followed-up for one-year and compared for the two groups,including maximal iuspiratory pressure (MIP),transdiaphragmatic pressure (Pdi),maximal transdiaphragmatic pressure (Pdimax),ratio of Pdi/Pdimax,arterial partial pressure of carbon dioxide (PaCO2),forced expiratory volume in one second (FEV1),6-min walking distance (6MWD),mortality and re-hospitalization rate.Results Age,gender,course of the disease,body mass index (BMI),arterial PaCO2,PaO2,MIP,Pdi,Pdiraax,ratio of Pdi/ Pdimax,FEV1,ratio of FEV1/FVC%,6MWD and re-hospitalization rate of the patients between the two groups were all comparable (P>0.05).In one-year follow-up,PaCO2averaged (52±8)mm Hg,MIP (64±7) cm H2O,Pdi (33±5) cm H2O,Pdimax (101±9) cm H2O,Pdi/Pdimax (0.31±0.04),FEV1 (35±4) %,FEV1/FVC% (44±4) %,6MWD (272±26) m and (2.6 ± 0.8) admissions per year in the HNPPV group,significantly different from those in the control group [ (57 ± 6) mm Hg,(59 ± 6) cm H2O,(31±4) cm H2O,(84±7) cm H2O,(0.35±0.05),(33±3)%,(41±4)%,(212±28) m,and (3.7±0.8) admissions per year] (P<0.05).One death was observed in the HNPPV group (1/24) and three in the control group (3/4 0) in one - year follow - up,with no statistically significant difference (X2=0.00,P>0.05).Conclusions Long-term use of HNPPV for patients with stable severe COPD could efficiently improve their respiratory muscle strength and endurance,thus improving their pulmonary ventilation and treatment efficcacy.

Objective To train health care workers (HCWs)by method of conventional training plus survey of hand contamination status,the influence of two kinds of methods in hand hygiene compliance of HCWs was evalua-ted.Methods From November 2013 to April 2014,all HCWs in a cardiovascular internal medicine department were as trained subjects,they were divided into two groups (trial group and control group).Hand hygiene compli-ance status was investigated 1 month before training.In the first month after training,conventional training method was adopted by both groups,from the second to fifth month,conventional training plus hand contamination survey was adopted by trial group,hand hygiene compliance between two groups were compared.Results Hand hygiene compliance rates of trial group and control group was 42.63% (107/251 )and 41 .80% (102/244)respectively be-fore training,there was no significant difference(P >0.05 ).In the first and second month after training,hand hygiene compliance rate of trial group was 55.70% (132/237)and 63.11 % (154/244)respectively,control group was 56.52% (130/230)and 62.61 % (149/238)respectively,compared with pre-training,the differences were sig-nificant (both P <0.05),but the difference was not significant between two groups(P >0.05);From the third to fifth month,hand hygiene compliance rates of trial group was 60.73%(150/247),61 .44%(145/236),and 61 .22%(150/245)respectively,control group was 51 .68%(123/238),51 .02%(125/245 ),and 52.32% (124/237)respec-tively,there was significant difference between two groups(P <0.05).Conclusion Conventional training combined with survey of hand contamination status can promote hand hygiene compliance of HCWs.

Objective To evaluate the clinical efficacy of a rapid 3D-printed patient-specific osteotomy guide in knee replacement surgery,and provide guidance for its widespread clinical application.Methods A total of 80 patients with end-stage knee osteoarthritis who had undergone Total Knee Arthroplasty(TKA)were selected and randomly divided into two groups.The first group comprised 40 cases(40 knees)that underwent traditional TKA,while the second group consisted of 40 patients(40 knees)in the 3D-printed osteotomy guide group.Various parameters,including surgery duration,intraoperative blood loss,time to ambulation after surgery,mechanical axis angle between femur and tibia post-surgery,Visual Analog Scale(VAS)score,Hospital for Special Surgery(HSS)knee score,Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC)score,range of motion(ROM)of the knee,and other indicators were analyzed separately for both groups.Statistical analyses were conducted.Results All the patients underwent surgery smoothly and were followed up for 3～12 months.In com-parison to the traditional osteotomy guide group,the 3D-printed osteotomy guide group demonstrated significantly shorter surgery duration(P<0.05),reduced intraoperative blood loss,earlier time to first ambulation after surgery(P<0.05),lower postoperative VAS score,and smaller mechanical axis angle between the femur and tibia after surgery(P<0.01).At 6 months post-surgery,both groups showed significantly improved KSS scores compared to preoperative values(P<0.01).The KSS score of the 3D-printed guide group was higher than that of the traditional surgical guide group at 6 months after surgery(P<0.05),but there was no statistical difference in KSS scores between the two groups(P>0.05).WOMAC scores for both groups decreased over time at 3 and 6 months post-surgery compared to preoperative scores(P<0.05).At these time points,WOMAC scores were lower in the 3D-printed guide group than in the traditional osteotomy guide group(P<0.05).One case of hematocele and infection occurred in the traditional osteotomy guide group;however,successful discharge was achieved following re-debridement while preserving the prosthesis.None of the surgical patients experienced complications such as neurovascular injury,deep vein thrombosis,prosthesis loosening or periprosthetic fractures.Conclusion Compared to conventional total knee arthroplasty(TKA)surgery,the utilization of a rapid 3D-printed osteotomy guide for knee replacement presents several advantages,including reduced surgical operation duration,minimal intraoperative blood loss,precise and expeditious osteotomies,accelerated postoperative recovery,and heightened patient satis-faction during medical consultations.Notably,its clinical efficacy surpasses that of traditional approaches.