Objective To investigate the correlation between occupational stress,uncertain endur-ance and job burnout of pre-hospital emergency nurses. Methods A total of 380 pre-hospital emergency nurses were recruited by stratified cluster sampling method.The occupational burnout scale(MBI)including emotional exhaustion(EE),depersonalization(D)and diminished personal accomplishment(PA),the In-tolerance of Uncertainty Scale(IUS),the Nursing Stressor Scale(NSS)including stresses associated with nurse expectations(NSS1),with family(NSS2),with interpersonal relationship(NSS3),with nature of work (NSS4),with patient(NSS5)and with work load(NSS6)were applied for all subjects.Results The pre-hospital emergency nurses who had worked less than 3 years got the higher EE score(33.58 ± 4.34)and higher PA score(26.98±4.31)than those who had worked 3 to 10 year(22.33±4.05,16.14±4.28,P<0.05) or longer than 10 years(21.50±4.36,16.81±4.36,P<0.05).Nurses got higher EE score(33.82±4.28,22.96 ±4.39,21.04±4.62,P<0.05),and higher PA scores(27.02±4.21,16.04±4.31,16.01±4.30,P<0.05)than the nurse practitioner or above levels.People whose monthly income were below 0.3 million got higher PA scores than those whose monthly income were 0.3 million or more(26.75±4.24,16.47±4.27,17.11±4.34,P<0.05).People who got degrees of bachelor or above had PA scores which was more than people who got de-grees under bachelor(26.33±4.36,17.24±4.28,P<0.05).Regression analysis showed that the multiplication score between IUS and NSS1 had the interaction effect with EE and D scores(β=0.33,0.33,P<0.05); the multiplication score between IUS and NSS5 had the interaction effect with EE and D scores(β=0.31,0.30, P<0.05)and the multiplication score between IUS and NSS3 had the interaction effect with D scores(β=0.34,P<0.05).Conclusion The pre-hospital emergency nurses'job burnout varies according to their length of service,job title,educational level and salary,and is also influenced by the combination of occupational stress and uncertain endurance.