Objective: To analyze the results of various methods to detect dengue virus(DENV) infection and to provide experience for the development of reasonable detection strategies on DENV infection. Methods: An acute phase case infected with type 2 DENV was used as an example to analyze various methods of detection and the relation of their results. Results: While NS1 antigen, IgM and IgG antibody were all negative, the nucleic acid test showed a positive result ; the NS1 coding gene of the DENV strain from the infected case did not mutate significantly; by additional use of NS1 reagents from other manufacturers, the antigen test result turned to weakly positive. Conclusions To conduct screening of dengue fever cases, the result of nucleic acid detection should also be applied. Moreover, additional use of NS1 reagents from other manufacturers could be helpful in reducing missed diagnosis, and could obtain more reliable result.