Objective To evaluate the feasibility and predictive ability of the sentinel node localization technique for patients with oral squamous cell carcinoma and determine whether the pathology of the SLN reflected the status of all the neck lymph nudes. Methods Perioperative use of a gamma probe identified radioactive SLNs. The pathology of the SLN was compared with that of the remaining neck dissection. Results Sentinel lymph node biopsy was performed on 20 cases. SLNs were found in 16 necks, among them 6 were found to be subclinical metastases. The SLN was the only lymph node containing tumor in 5 of 6. Conclusions Our preliminary investigation shows that sentinel node localization is technically feasible and predictive of cervical metastasis. SLN biopsy may aid in identifying the clinically N0 patient with occult lymph node metastasis and has the potential to decrease the number of neck dissections performed in clinically negative necks.

Objective To evaluate the sentinel lymph node biopsy (SLNB) in clinical lymph node negative oral squamous cell carcinoma. Methods Sentinel lymph node biopsy (SLNB) by using methylene blue staining were employed to 20 patients with clinical cervical lymph node negative in oral squamous cell carcinoma. Results SLNB was successful identified in 14 of 20(70%). A 100%(6/6) accuracy of SLNB to predict cervical lymph node status was reached. Conclusion SLNB could accurately predict the cervical status of clinical negative node and decrease the extent of surgery and postoperative complication

cases of Warthin tumor in parotid gland were collected, the selection of operating style and personal experience in treatment were introduced. The results showed that most of the patients were senior man, the site of the tumors was all in parotid gland, the majority of the tumor was single nodule with exception of some patient who had a multiple nature. Tumor recurrence was found in patients with improper method. The results suggested that for patients who are suspected of Warthin tumor,careful physical examination and B Ultrosonic scan are necessary for both sides of parotid gland. Different operating style should be considered according to the differences of tumor site and number.

By using digoxin labelled human papillomavirus(HPV) 6/11 and HPV16/18 probes,and hybridization in situ technique, the HPV DNA sequence in 50 cases of oral SCC was detected. The results showed that among them ,16 cases(32%) were positive for HPV16/18 DNA, none of the cases of OSCC was positive for HPV6/11 DNA. It was suggested that HPV16/18 in oral SCC was confirmed to be closely related with the cause of OSCC.

To explore the characteristics and treatment of pseudopericoronitis of wisdom teeth of the mandible by clinical analysis of 36 cases.Results showed that the patients of pseudopericoronitis of the wisdom tooth of the mandible were of elder age,mild symptoms, totally impacted in the mandible,resulting in overgrowth of the corresponding third molar of the maxtilla overgrown.The observations indicated that the symptoms of pseudocoronitis of the wisdom tooth of the mandible was the injury inflicted to the soft tissue due to excessive growth of the third molar in the maxilla directly or indirectly.The method of treatment is to extract the overgrown third molar in the maxilla,and leave the wisdom tooth of the mandible alone.

OBJECTIVETo improve the recognization and treatment for oral venous lakes (OVL).

METHODSClinicopathologically analysis of 20 cases of oral venous lakes was performed. All the samples were removed by surgically.

RESULTSAll the lesions occurred in senile persons and located under the mucosa. Among the 20 cases, 11 were male patients and 9 were female; 18 of 20 occurred in the lower lip, another two in the upper lip and buccal mucosa. The lesions were 0.3 approximately 1.2 cm in size. Pathologically, the oral venous lake was composed of single or a few large, dilated and irregular veins. Thrombosis and organization were found within the lesions sometimes.

CONCLUSIONSOral venous lakes are a kind of focal anomalies of venous structure. All the 20 cases (with a follow-up time from 8 months to 4 years) has no recurrence after surgical treatment.

Aged ; Diagnosis, Differential ; Female ; Humans ; Lip Diseases ; diagnosis ; pathology ; surgery ; Male ; Middle Aged ; Mouth Mucosa ; pathology ; Vascular Diseases ; pathology ; surgery

New bone formation in long-term intramuscle implant of Ca-P biomaterial was investigated in this experiment. After implanting into dog dorsal muscle for 15 months, a thin fibrous membrane that wrapped HA/TCP implant was still observed obviously. Three types of tissues, i.e. mesenchymal tissue, bone and bone marrow, regularly distributed in different pores of implant. Nearly all the pores of implants were occupied by bone. Bone in the pores located in the central region of implant was matured lamellar bone characterized by obvious lacuna and rich bone marrow. However, bone in the peripheral pores was immature woven bone without bone marrow formation. Furthermore, mesenchymal tissues only exist in the peripheral pores and usually were connected with immature woven bone. It was demonstrated that porous HA/TCP has bone inductivity and it could induce new bone formation at non-osseous site. Well-regulated distribution of mesenchymal tissue, bone and bone marrow in the pores suggest bone morphogenesis in the implant must obey a specific space-time program.
Animals ; Biocompatible Materials ; Bone Substitutes ; pharmacology ; Ceramics ; pharmacology ; Dogs ; Hydroxyapatites ; pharmacology ; Implants, Experimental ; Materials Testing ; Osteogenesis ; drug effects

Human tissue typing methods were employed in developing a porcine allotransplantation model. 23 Chinese Sichuan White Pigs(2-3 months old, 17.5+/-4.6kg, with clear family background) were selected for tissue typing, ABO blood type cross reaction, complement-dependent cytotoxicity (CDC) cross reaction and one way mixed lymphocyte reaction (MLR). 6 pairs of swine that showed better matching results were selected as donors and recipients. Single-kidney orthotopic transplantation was conducted after removing both kidneys of the recipient. Five recipients showed low matching results (MLR ranging from 2175 to 3560, CDC from 1 to 4); of them, 2 died of operation, 2 died of acute renal tubular necrosis and accelerating rejection 4 days after operation respectively, and 1 died of acute renal tubular necrosis 4 days after operation. 6 recipients showed high matching results (MLR ranging from 982 to 1916, CDC from 2 to 4); of them, 1 died of anaesthesia during operation, 3 died of accelerating rejection and acute rejection 2 weeks after operation respectively, 1 had good kidney function, and 1 presented weak rejection 1 week after operation but the kidney function came back to normal afterwards. Human tissue typing methods could be adopted in developing the porcine model. Hyperacute rejection could be avoided by screening ABO blood type, CDC and MLR tests. However, based on these primary data, it was hard to evaluate the predictive values of CDC and one-way MLR for accelerating rejection, acute rejection and graft chronic dysfunction. Further research by expanding experiments in these aspects is still going on.
ABO Blood-Group System ; immunology ; Animals ; Cytotoxicity Tests, Immunologic ; Graft Rejection ; prevention & control ; Histocompatibility Testing ; Kidney Transplantation ; immunology ; Swine ; T-Lymphocytes ; immunology ; Transplantation, Homologous ; immunology

Rabbit bone marrow-derived mesenchymal stem cells(MSCs) are multipotent. We studied the adipogenic and osteogenic differentiation potent using adipogenic supplement (AS) or osteogenic supplement (OS) in vitro. Specific markers of this induced adipogenic and osteogenic lineage were identified. The findings showed that the rabbit MSCs are capable of differentiating into adipogenic and osteogenic lineages spontaneously. On the 21st day, approximately 75% rabbit MSCs were induced to adipogenic or osteogenic cells in medium containing AS or OS, respectively. These results demonstrated that the differentiation of MSCs could be regulated in vitro. The underlying molecular mechanisms of adipogenic or osteogenic differentiation await elucidation.
Adipose Tissue ; cytology ; Animals ; Bone and Bones ; cytology ; Cell Differentiation ; Cell Lineage ; In Vitro Techniques ; Mesoderm ; cytology ; Rabbits ; Stem Cells ; cytology